Our Anti-ERK/MAPK (Thr202/Tyr204) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat ERK/MAPK (Thr202/Tyr204) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, ICC.
Primary Antibody
Human, Mouse, Rat
Bovine, Canine, Chicken, Non-Human Primate, Xenopus, Zebrafish
ICC, IHC, WB
Rabbit
Antigen Affinity Purified from Pooled Serum
MAPK1/2
42/44 kDa




Immunostaining of neurons in the frontal cortex of saline treated mouse brain identifying cytoplasmic and nuclear staining of ERK/MAPK when phosphorylated at Thr202/Tyr204 (cat. p160-2024, red, 1:500). The intense nuclear staining of a few neurons shows stimulation of the neuron resulting in translocation of the protein. The blue is staining nuclei with DAPI. Photo courtesy of Robert Wine.
Product Specific References for Applications and Species
Immunocytochemistry: Human | ||
PMID | Dilution | Publication |
27743527 | 1:250 | Erickson, C.A., et al. 2019. Initial analysis of peripheral lymphocytic extracellular signal related kinase activation in autism. Journal of Psychiatric Research, 84, pp.153-160. |
Immunocytochemistry: Mouse | ||
PMID | Dilution | Publication |
32841711 | 1:100 | Castellanos, L.C.S., et al. 2020. Upregulation of ASIC1a channels in an in vitro model of Fabry disease. Neurochemistry International, 140, p.104824. |
Immunohistochemistry: Human | ||
PMID | Dilution | Publication |
22765220 | 1:100 | Iwanga, R., et al. 2012. Expression of Six1 in luminal breast cancers predicts poor prognosis and promotes increases in tumor initiating cells by activation of extracellular signal-regulated kinase and transforming growth factor-beta signaling pathways. Breast Cancer Research, 14(4), p.R100. |
Western Blot: Human | ||
PMID | Dilution | Publication |
27743527 | 1:250 | Erickson, C.A., et al. 2017. Initial analysis of peripheral lymphocytic extracellular signal related kinase activation in autism. Journal of Psychiatric Research, 84, pp.153-160. |
26181363 | not listed | Morgan, J.E., et al. 2015. The class II transactivator (CIITA) is regulated by post-translational modification cross-talk between ERK1/2 phosphorylation, mono-ubiquitination and Lys63 ubiquitination. Bioscience Reports, 35(4). |
Western Blot: Mouse | ||
PMID | Dilution | Publication |
29242167 | 1:100 | Rainville, J.R., et al. 2019. Membrane-initiated nuclear trafficking of the glucocorticoid receptor in hypothalamic neurons. Steroids<>/i1,142:55-64. |