Anti-ERK/MAPK (Thr202/Tyr204) Antibody

from PhosphoSolutions Datasheet Trial Size Trial Size Pooled Serum Pooled Serum
6 Citations

Our Anti-ERK/MAPK (Thr202/Tyr204) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat ERK/MAPK (Thr202/Tyr204) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, ICC.

Primary Antibody
Human, Mouse, Rat
Bovine, Canine, Chicken, Non-Human Primate, Xenopus, Zebrafish
ICC, IHC, WB
Rabbit
Antigen Affinity Purified from Pooled Serum
MAPK1/2
42/44 kDa

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Immunostaining of neurons in the frontal cortex of saline treated mouse brain identifying cytoplasmic and nuclear staining of ERK/MAPK when phosphorylated at Thr202/Tyr204 (cat. p160-2024, red, 1:500). The intense nuclear staining of a few neurons shows stimulation of the neuron resulting in translocation of the protein. The blue is staining nuclei with DAPI. Photo courtesy of Robert Wine.
Immunostaining of granule cells in the dentate gyrus of the hippocampus from saline treated mouse brain staining ERK/MAPK when phosphorylated at Thr202/Tyr204 (cat. p160-2024, 1:500, red). The blue is staining nuclei with DAPI. The MAPK positive neurons show punctate staining primarily localized in the nucleus with few staining both cytoplasmic and nuclear. Photo courtesy of Robert Wine.
Immunolabeling of cultured mouse hippocampal neurons fixed and stained with anti-phospho-ERK/MAPK Thr202/Tyr204 (p160-2024, green, 1:100) and red nuclear stain Propidium Iodide. The labeling identifies an increase in ERK/MAPK phosphorylation when hippocampal neurons are treated with a specific ASIC1a activator, MitTx toxin (20 nM, 4 min). Image kindly provided by Carina Weissmann, IFIBYNE-CONICET.
Western blot of human T47D cell lysate showing specific immunolabeling of ~42-44 kDa ERK/MAPK protein phosphorylated at Thr202/Tyr204 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).
Immunostaining of neurons in the frontal cortex of saline treated mouse brain identifying cytoplasmic and nuclear staining of ERK/MAPK when phosphorylated at Thr202/Tyr204 (cat. p160-2024, red, 1:500). The intense nuclear staining of a few neurons shows stimulation of the neuron resulting in translocation of the protein. The blue is staining nuclei with DAPI. Photo courtesy of Robert Wine.
Immunostaining of neurons in the frontal cortex of saline treated mouse brain identifying cytoplasmic and nuclear staining of ERK/MAPK when phosphorylated at Thr202/Tyr204 (cat. p160-2024, red, 1:500). The intense nuclear staining of a few neurons shows stimulation of the neuron resulting in translocation of the protein. The blue is staining nuclei with DAPI. Photo courtesy of Robert Wine.
Immunostaining of granule cells in the dentate gyrus of the hippocampus from saline treated mouse brain staining ERK/MAPK when phosphorylated at Thr202/Tyr204 (cat. p160-2024, 1:500, red). The blue is staining nuclei with DAPI. The MAPK positive neurons show punctate staining primarily localized in the nucleus with few staining both cytoplasmic and nuclear. Photo courtesy of Robert Wine.
Immunolabeling of cultured mouse hippocampal neurons fixed and stained with anti-phospho-ERK/MAPK Thr202/Tyr204 (p160-2024, green, 1:100) and red nuclear stain Propidium Iodide. The labeling identifies an increase in ERK/MAPK phosphorylation when hippocampal neurons are treated with a specific ASIC1a activator, MitTx toxin (20 nM, 4 min). Image kindly provided by Carina Weissmann, IFIBYNE-CONICET.
Western blot of human T47D cell lysate showing specific immunolabeling of ~42-44 kDa ERK/MAPK protein phosphorylated at Thr202/Tyr204 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (λ-Ptase, 1200 units for 30 min).

Anti-ERK/MAPK (Thr202/Tyr204) Antibody
SKU: p160-2024

Volume: 100 µL
Price:
$415

Bulk Order Anti-ERK/MAPK (Thr202/Tyr204) Antibody

Product Specific References for Applications and Species

Immunocytochemistry: Human
PMID Dilution Publication
27743527 1:250 Erickson, C.A., et al. 2019. Initial analysis of peripheral lymphocytic extracellular signal related kinase activation in autism. Journal of Psychiatric Research, 84, pp.153-160.
Immunocytochemistry: Mouse
PMID Dilution Publication
32841711 1:100 Castellanos, L.C.S., et al. 2020. Upregulation of ASIC1a channels in an in vitro model of Fabry disease. Neurochemistry International, 140, p.104824.
Immunohistochemistry: Human
PMID Dilution Publication
22765220 1:100 Iwanga, R., et al. 2012. Expression of Six1 in luminal breast cancers predicts poor prognosis and promotes increases in tumor initiating cells by activation of extracellular signal-regulated kinase and transforming growth factor-beta signaling pathways. Breast Cancer Research, 14(4), p.R100.
Western Blot: Human
PMID Dilution Publication
27743527 1:250 Erickson, C.A., et al. 2017. Initial analysis of peripheral lymphocytic extracellular signal related kinase activation in autism. Journal of Psychiatric Research, 84, pp.153-160.
26181363 not listed Morgan, J.E., et al. 2015. The class II transactivator (CIITA) is regulated by post-translational modification cross-talk between ERK1/2 phosphorylation, mono-ubiquitination and Lys63 ubiquitination. Bioscience Reports, 35(4).
Western Blot: Mouse
PMID Dilution Publication
29242167 1:100 Rainville, J.R., et al. 2019. Membrane-initiated nuclear trafficking of the glucocorticoid receptor in hypothalamic neurons. Steroids<>/i1,142:55-64.

Product Specific Protocols

  • ERK/MAPK Thr202/Tyr204 ICC Protocol: Download
  • ERK/MAPK Thr202/Tyr204 IHF Protocol: Download

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