Double C2-like domain-containing protein beta or Doc2b is encoded by the gene DOC2b. Doc2b functions as a calcium sensor which positively regulates soluble NSF attachment receptor (SNARE) dependent fusion of vesicles with membranes. Doc2b binds phospholipids and may act at the priming stage of membrane fusion by modifying membrane curvature to stimulate fusion. Doc2b is expressed in pancreatic cells, endocrine cells and brain, where it is highly expressed in neurons. (Friedrich et al.Neurosci., July 2, 2008 • 28(27):6794 – 6806)
ICC, IHC, WB
Fusion protein amino acids 1-216 (N-terminus) of rat Doc2b (also known as Double C2-like domain-containing protein beta and Doc2-beta, accession number P70610); Mouse: 97% identity (210/216 amino acids identical); Human: 93% identity (203/169 amino acids identical); Less than 60% identity with Doc2a
Store at ≤ -20 C for long term storage. For short term storage, store at 2-8 C. For maximum recovery of product, centrifuge the vial prior to removing the cap.