Anti-Coronin-1A Antibody

Name: Anti-Coronin-1A Antibody
Brand: Biosensis
SKU: R-1335-50
Price: 327.0 USD
Availability: InStock

Our Anti-Coronin-1A rabbit polyclonal primary antibody detects bovine, human, mouse, pig, and rat Coronin- 1A, and is whole serum. It is validated for use in ICC, WB.

Immunocytochemistry of a mixed neuron/glial culture from newborn rat brain stained with Rabbit polyclonal antibody to Coronin 1a R-1335-50 (green) at a dilution of 1:10,000 and Chicken polyclonal antibody to Vimentin C-1409-50 at 1:5,000. Blue is nuclear DNA counter stain. Glial cells and fibroblasts stain with Vimentin, while microglia alone stain strongly and specifically for Coronin 1a, which can therefore be used as a robust marker of this important cell type.

Left: Detection of Coronin 1a (red) in cortical neuron-glial cell culture from E20 rat by Immunocytochemistry. Coronin 1a antibody was used at 1:1,000 dilution. Cells were co-stained with mouse antibody to GFAP (M-1375-100, green, 1:1,000). Blue: Hoechst nuclear stain. The coronin 1a antibody labels protein expressed in the cytoplasm of microglia cells, while the GFAP antibody stains intermediate filaments in astrocytic cells. Right: Western blot analysis of coronin 1a expression in tissue lysates using rabbit anti-coronin 1a antibody (green, 1:5,000). [1] protein standard, [2] mouse brain, [3] rat brain, [4] cow cerebellum, [5] cow cortex, and [6] pig spinal cord. The strong single band about 55 kDa corresponds to the coronin 1a protein.

Immunocytochemistry of a mixed neuron/glial culture from newborn rat brain stained with Rabbit polyclonal antibody to Coronin 1a R-1335-50 (green) at a dilution of 1:10,000 and Chicken polyclonal antibody to Vimentin C-1409-50 at 1:5,000. Blue is nuclear DNA counter stain. Glial cells and fibroblasts stain with Vimentin, while microglia alone stain strongly and specifically for Coronin 1a, which can therefore be used as a robust marker of this important cell type.

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Species Reactivity

Bovine, Human, Mouse, Pig, Rat

Applications

ICC, WB

Host Species

Rabbit

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Data Sheet

SKU: R-1335-50

Volume/Size: 50 µg

Variant

Price:

$327.00

Quantity:

Product Details

Target

Coronin-1A

Scientific Background

Coronins belong to the WD40 or WD family of proteins. Coronins appear to be particularly involved in binding to actin, actin associated proteins, tubulin and phospholipase C and have been implicated in the mechanisms of chemotaxis and phagocytosis. In mammals there are at least five major coronin proteins, named coronins 1 to 5 in one nomenclature. Another nomenclature divides these five proteins in coronins 1a and 1b, 2a, 2b and 2c (see the Human Genone Organization Gene Nomenclature Committee link for this family). The mammalian coronin family members are abundant components of eukaryotic cells and each type has a restricted cell type specific expression pattern. Coronin 1A is found exclusively in hematopoetic lineage cells such as lymphocytes, macrophages and neutrophils. This antibody is therefore an excellent marker of cells of this lineage and can also be used to study the leading edges particularly of neutrophils. Since the only hematopoetic cells found within the central nervous system are microglia, this antibody is also an excellent marker of this important cell type. Microglia are numerically fairly minor components of the nervous system, but microglial activation is seen in response to a wide variety of damage and disease states, including ALS, Alzheimer's disease and responses to brain tumors. Since coronin 1a is a constitutive component of microglia, the coronin 1a antibody can be used to study both quiescent and activated microglia.

Format

Whole serum

Clonality

Polyclonal

Isotype

Mixed

Applications

ICC, WB

Host Species

Rabbit

Molecular Weight

51 kDa

Immunogen

C-terminal peptide of human coronin 1a coupled to KLH

Immunogen Species

Human

Species Reactivity

Bovine, Human, Mouse, Pig, Rat

Storage

Spin vial briefly before opening. Reconstitute with 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.

Physical State

Lyophilized

Buffer

Lyophilized with sodium azide.

Dilution Ranges

WB: 1:2500-1:5000
IP: 1:500-1:1000

Conjugate

Unconjugated

Specificity

The specificity of this antibody has been confirmed by WB and ICC against the antigen. Human, rat, mouse and feline. Predicted to react with other mammalian tissue.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

Coronin-1A; Coronin-like protein A; Clipin-A; Coronin-like protein p57; Tryptophan aspartate-containing coat protein; TACO; CORO1A; CORO1;

UniProt ID

P31146

Shipping

25°C (ambient)

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Anti-Coronin-1A Antibody

SKU: R-1335-50

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