Our Anti-Cav1.3 Ca2+ channel mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N38/8. It detects human, mouse, and rat Cav1.3 Ca2+ channel, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.
Immunostaining of mouse brain cortex identifying the calcium channels along axons stained with Anti-Cav1.3 Ca2+ Channel Antibody (Cat 75-080, 1:500, green) and nuclei are labeled with DAPI (blue). Image kindly provided by Kaveh Moradi, Virginia Commonwealth University.
Voltage-dependent L-type calcium channel subunit alpha-1D, or Cav1.3 calcium channel is a calcium channel encoded by the gene CACNA1D. It is a member of the L type voltage dependent calcium channel family. These calcium channels mediate the influx of calcium ions into a cell upon membrane polarization. Cav1.3 is expressed primarily in the membrane of the heart, brain (cerebral cortex, hippocampus, basal ganglia and thalamus). and pancreatic islets. Calcium channels are involved in many cell processes including muscle contraction, neurotransmitter release, gene expression, cell division and cell death. Diseases associated with Cav1.3 include Primary Aldosteronism, Seizures, Sinoatrial Node Dysfunction and Deafness.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
N38/8
IgG1
ICC, IHC, IP, WB
Mouse
Cacna1d Cach3 Cacn4 Cacnl1a2 Cchl1a2
>220 kDa
Fusion protein amino acids 2025-2161 (C-terminus) of rat Cav1.3 (accession number P27732) produced recombinantly in E. Coli
Human, Mouse, Rat
AB_10673964
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.10
WB: 1:1000
IHC: 1:100
ICC: 1:100
Unconjugated
Does not cross-react with Cav1.2
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Liedtke, W., et al. 2013. Highly conductive carbon nanotube matrix accelerates developmental chloride extrusion in central nervous system neurons by increased expression of chloride transporter KCC2. Small (Weinheim an der Bergstrasse, Germany), 1066-1075.
Liedtke, W., et al. 2013. Highly conductive carbon nanotube matrix accelerates developmental chloride extrusion in central nervous system neurons by increased expression of chloride transporter KCC2. Small (Weinheim an der Bergstrasse, Germany), 1066-1075.
Hurley, M., et al. 2013. Parkinson's disease is associated with altered expression of CaV1 channels and calcium-binding proteins. Brain : a journal of neurology, 2077-2097.
Bernstein, S., et al. 2009. Expressed sequence tag analysis of adult human optic nerve for NEIBank: identification of cell type and tissue markers. BMC neuroscience, 121.
Radhakrishnan, V., et al. 2017. Expression of Cav1.3 calcium channel in the human and mouse colon: posttranscriptional inhibition by IFNγ. American journal of physiology. Gastrointestinal and liver physiology, G77-G84.
Hurley, M., et al. 2013. Parkinson's disease is associated with altered expression of CaV1 channels and calcium-binding proteins. Brain : a journal of neurology, 2077-2097.
Bernstein, S., et al. 2009. Expressed sequence tag analysis of adult human optic nerve for NEIBank: identification of cell type and tissue markers. BMC neuroscience, 121.
Radhakrishnan, V., et al. 2017. Expression of Cav1.3 calcium channel in the human and mouse colon: posttranscriptional inhibition by IFNγ. American journal of physiology. Gastrointestinal and liver physiology, G77-G84.
Goonasekera, S., et al. 2012. Decreased cardiac L-type Ca²⁺ channel activity induces hypertrophy and heart failure in mice. The Journal of clinical investigation, 280-290.
Schierberl, K., et al. 2011. Cav1.2 L-type Ca²⁺ channels mediate cocaine-induced GluA1 trafficking in the nucleus accumbens, a long-term adaptation dependent on ventral tegmental area Ca(v)1.3 channels. The Journal of neuroscience : the official journal of the Society for Neuroscience, 13562-13575.
Bissig, B., et al. 2013. Diminished vision in healthy aging is associated with increased retinal L-type voltage gated calcium channel ion influx. PloS one, e56340.
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