Anti-Ataxin-1, WT Antibody (N65/37)

Name: Anti-Ataxin-1, WT Antibody
Brand: NeuroMab
SKU: 75-102
Price: 319.0 USD
Availability: InStock

Our Anti-Ataxin-1, WT mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N65/37. It detects human, mouse, and rat Ataxin-1, WT, and is purified by Protein A chromatography. It is great for use in ICC, WB.

Adult rat hippocampus immunohistochemistry.

Transfected cell immunoblot: extracts of COS-1 cells transfected with Ataxin- 1-WT, Ataxin-1-S751A or Kv2.1 plasmid and probed with N65/37 TC supe.

Adult rat cerebellum immunohistochemistry.

Adult rat hippocampus immunohistochemistry.

Species Reactivity

Human, Mouse, Rat

Applications

ICC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

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Data Sheet

SKU: 75-102

Volume: 100 µL

Variant

Price:

$319.00

Quantity:

Product Details

Target

Ataxin-1, WT

Target Description

Ataxin1, also known as spinocerebellar ataxia type 1 protein homolog, is a highly conserved DNA-binding protein. Ataxin1 is expressed in brain and can be found at high levels in the cortex and the hypothalamus in neurons. It is also expressed in other tissues. Within the cell, it is highly expressed in the nucleus, and can also be found in the cytoplasm. Mutations in the ataxin-1 gene cause spinocerebellar ataxia type 1.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N65/37

Isotype

IgG1

Applications

ICC, WB

Host Species

Mouse

Gene Name

Atxn1 Sca1

Molecular Weight

85 kDa

Antigen

Synthetic peptide amino acids 746-761 (RKRRRWSAPETRKLEK) of mouse ataxin-1 (accession number P54254)

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_10675132

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.30

Conjugate

Unconjugated

Specificity

Minimal cross-reactivity against S751A mutant of Ataxin-1 by ELISA and immunofluorescence and negative by immunoblot (right)

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Ataxin-1 (Spinocerebellar ataxia type 1 protein homolog)

UniProt Details

UniProt (Human): P54253
UniProt (Immunogen Species): P54254

Product Specific References for Applications and Species

Immunocytochemistry: Human
Immunohistochemistry: Human

Immunocytochemistry: Human
PMID	Dilution	Publication
23935513	not listed	Menon, R.P., et al. 2013. The role of interruptions in polyQ in the pathology of SCA1.. PLOS Genetics, e1003648.

Immunohistochemistry: Human
PMID	Dilution	Publication
26616331	1:500	Ruan, K., et al. 2015. Alternative splicing of Drosophila Nmnat functions as a switch to enhance neuroprotection under stress.. Nature Communications, 10057.

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Anti-Ataxin-1, WT Antibody (N65/37)

SKU: 75-102

Product Details

Product Specific References for Applications and Species

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