Anti-Aquaporin 2 (Ser261) Antibody

Name: Anti-Aquaporin 2 (Ser261) Antibody
Brand: PhosphoSolutions
SKU: p112-261
Price: 430.0 USD
Availability: InStock

24 Citations

Our Anti-Aquaporin 2 (Ser261) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Aquaporin 2 (Ser261) and is antigen affinity purified from pooled serum. It is great for use in WB, IHC, ICC, IP.

Western blot of rat kidney lysate showing specific immunolabeling of the ~29 kDa and 37 kDa glycosylated form of the AQP2 protein phosphorylated at Ser261 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding non-phosphopeptide (not shown).

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, IP, WB

Host Species

Rabbit

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Data Sheet

Trial Size

Pooled Serum

SKU: p112-261

Volume: 100 µL

Variant

Price:

$430.00

Quantity:

Product Details

Target

Aquaporin 2 (Ser261)

Target Description

Aquaporin 2 (AQP2) is a hormonally regulated water channel located in the renal collecting duct. Mutations in the AQP2 gene cause hereditary nephrogenic diabetes insipidus in humans (Iolascon et al.,2007). A vasopressin induced cAMP increase results in the phosphorylation of AQP2 at serine-256 and its translocation from the intracellular vesicles to the apical membrane of principal cells (van Balkom et al., 2002). Recently, serine-261 has been identified as a novel phosphorylation site on AQP2 and levels of phosphorylated S261 have been shown to decrease with vasopressin treatment suggesting its involvement in vasopressin-dependent AQP2 trafficking (Hoffert et al., 2007)

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, IHC, IP, WB

Host Species

Rabbit

Gene Name

AQP2

Molecular Weight

29/37 kDa

Antigen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser261 of rat aquarporin 2, conjugated to keyhole limpet hemocyanin (KLH).

Species Reactivity

Human, Mouse, Rat

Expected Reactivity

Bovine, Canine, Chicken, Non-Human Primate

Antibody Registry ID

AB_2492041

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
IHC: 1:2000
ICC: 1:500-1:3000
IP: 3 ul

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~29 kDa AQP2 protein phosphorylated at Ser261. Also recognizes the glycosylated form of AQP2 at ~37 kDa. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Post Translational Modification Type

Phosphorylated

Post Translational Modification Residue

Ser261

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Shipping

Blue Ice

Synonyms

ADH water channel antibody, AQP 2 antibody, AQP CD antibody, AQP-2 antibody, AQP-CD antibody, AQP2 antibody, AQP2_HUMAN antibody, AQPCD antibody, Aquaporin 2 collecting duct antibody, Aquaporin CD antibody, Aquaporin-2 antibody, Aquaporin-CD antibody, Aquaporin2 antibody, Aquaporine 2 antibody, Collecting duct water channel protein antibody, MGC34501 antibody, Water channel aquaporin 2 antibody, Water channel protein for renal collecting duct antibody, WCH CD antibody, WCH-CD antibody, WCHCD antibody

UniProt Details

UniProt (Human): P41181
UniProt (Immunogen Species): P34080

NCBI Gene ID

25386

Product Specific References for Applications and Species

Immunocytochemistry: Rat
Immunohistochemistry: Mouse Rat
Immunoprecipitation: Mouse Rat
Western Blot: Human | Mouse | Rat

Immunocytochemistry: Rat
PMID	Dilution	Publication
28668390	1:500	Yui, N., et al. 2017. Ser-261 phospho-regulation is involved in pS256 and pS269-mediated aquaporin-2 apical translocation. Biochemical and Biophysical Research Communications. Aug 26;490(3):1039-1044.
27760768	1:3000	Mamuya, F.A., et al. 2016. ILK and Cytoskeletal Architecture: An Important Determinant of AQP2 Recycling and Subsequent Entry into the Exocytotic Pathway. American Journal of Physiology-Renal Physiology, pp.ajprenal-00336.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
18606813	1:2000	Hoffert, J.D., et al. 2008. Vasopressin-stimulated increase in phosphorylation at serine 269 potentiates plasma membrane retention of aquaporin 2. J Biol Chem. 2008 Sep 5; 283(36):24617-27.
16985212	1:2000	Hoffert, J.D., et al. 2007. Dynamics of aquaporin-2 serine-261 phosphorylation in response to short-term vasopressin treatment in collecting duct. Am J Physiol Renal Physiol 292: F691-F700.

Immunohistochemistry: Rat
PMID	Dilution	Publication
26147297	not listed	Rice, W.L., et al. 2015. Polarized Trafficking of AQP2 Revealed in Three Dimensional Epithelial Culture. PloS one, 10(7), e0131719.
18606813	1:2000	Hoffert, J.D., et al. 2008. Vasopressin-stimulated increase in phosphorylation at serine 269 potentiates plasma membrane retention of aquaporin 2. J Biol Chem. 2008 Sep 5; 283(36):24617-27.
16985212	1:2000	Hoffert, J.D., et al. 2007. Dynamics of aquaporin-2 serine-261 phosphorylation in response to short-term vasopressin treatment in collecting duct. Am J Physiol Renal Physiol 292: F691-F700.

Immunoprecipitation: Mouse
PMID	Dilution	Publication
27889609	3 uL	Yui, N., et al. 2017. Aquaporin-2 Ser-261 phosphorylation is regulated in combination with Ser-256 and Ser-269 phosphorylation. Biochemical and biophysical research communications, 482(4), pp.524-529.

Immunoprecipitation: Rat
PMID	Dilution	Publication
27889609	3 uL	Yui, N., et al. 2017. Aquaporin-2 Ser-261 phosphorylation is regulated in combination with Ser-256 and Ser-269 phosphorylation. Biochemical and biophysical research communications, 482(4), pp.524-529.

Western Blot: Human
PMID	Dilution	Publication
32529500	not listed	Sakai, M., et al. 2020. Phosphorylation profile of human AQP2 in urinary exosomes by LC–MS/MS phosphoproteomic analysis. Clinical and Experimental Nephrology, pp.1-8.

Western Blot: Mouse
PMID	Dilution	Publication
32529500	not listed	Sakai, M., et al. 2020. Phosphorylation profile of human AQP2 in urinary exosomes by LC–MS/MS phosphoproteomic analysis. Clinical and Experimental Nephrology, pp.1-8.
29046300	1:3000	Lei, L., et al. 2018. Manganese promotes intracellular accumulation of AQP2 via modulating F-actin polymerization and reduces urinary concentration in mice. American Journal of Physiology-Renal Physiology, Oct 18: ajprenal-00391.
27889609	1:500-1:1000	Yui, N., et al. 2017. Aquaporin-2 Ser-261 phosphorylation is regulated in combination with Ser-256 and Ser-269 phosphorylation. Biochemical and biophysical research communications, 482(4), pp.524-529.
27760768	1:3000	Mamuya, F.A., et al. 2016. ILK and Cytoskeletal Architecture: An Important Determinant of AQP2 Recycling and Subsequent Entry into the Exocytotic Pathway. American Journal of Physiology-Renal Physiology, pp.ajprenal-00336.
18606813	1:2000	Hoffert, J.D., et al. 2008. Vasopressin-stimulated increase in phosphorylation at serine 269 potentiates plasma membrane retention of aquaporin 2. J Biol Chem. 2008 Sep 5; 283(36):24617-27.
16985212	1:2000	Hoffert, J.D., et al. 2007. Dynamics of aquaporin-2 serine-261 phosphorylation in response to short-term vasopressin treatment in collecting duct. Am J Physiol Renal Physiol 292: F691-F700.

Western Blot: Rat
PMID	Dilution	Publication
29046300	1:3000	Lei, L., et al. 2018. Manganese promotes intracellular accumulation of AQP2 via modulating F-actin polymerization and reduces urinary concentration in mice. American Journal of Physiology-Renal Physiology, Oct 18:ajprenal-00391.
28668390	1:500	Yui, N., et al. 2017. Ser-261 phospho-regulation is involved in pS256 and pS269-mediated aquaporin-2 apical translocation. Biochemical and Biophysical Research Communications. Aug 26; 490(3):1039-1044.
27889609	1:500-1:1000	Yui, N., et al. 2017. Aquaporin-2 Ser-261 phosphorylation is regulated in combination with Ser-256 and Ser-269 phosphorylation. Biochemical and biophysical research communications, 482(4), pp.524-529.
27534994	not listed	Al-Butaineh, M.M, et al. 2016. Activation of the Metabolic Sensor AMP-Activated Protein Kinase Inhibits Aquaporin-2 Function in Kidney Principal Cells. American Journal of Physiology-Renal Physiology, ajprenal-00308.
27185292	not listed	Speed, J.S, et al. 2016. In vivo organ specific drug delivery with implantable peristaltic pumps. Scientific reports, 6, p.26251.
26147297	not listed	Rice, W.L., et al. 2015. Polarized Trafficking of AQP2 Revealed in Three Dimensional Epithelial Culture. PloS one, 10(7), e0131719.
18606813	1:2000	Hoffert, J.D., et al. 2008. Vasopressin-stimulated increase in phosphorylation at serine 269 potentiates plasma membrane retention of aquaporin 2. J Biol Chem. 2008 Sep 5; 283(36):24617-27.
16985212	1:2000	Hoffert, J.D., et al. 2007. Dynamics of aquaporin-2 serine-261 phosphorylation in response to short-term vasopressin treatment in collecting duct. Am J Physiol Renal Physiol 292: F691-F700.

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Product Specific Protocols

Aquaporin ser261 IHF Protocol: Download

Western Blot Protocol: Download

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Anti-Aquaporin 2 (Ser261) Antibody

SKU: p112-261

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-Aquaporin 2 (Ser261) Antibody

Product Specific Protocols

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