Products: Primary Antibodies

Anti-Ankyrin-R Antibody (N388A/10)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-380	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $285.00
Brand	Catalog # 73-380	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $285.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2b

Species Reactivity:

Human Mouse Rat

Applications:

ICC IHC WB

Target Description:

Ankyrin-1 or -Ankyrin-R is encoded by the gene ANK1. Ankyrin R is a scaffold protein that functions to link proteins that mediate the attachment of integral membrane proteins to the spectrin-actin based membrane cytoskeleton. In this manner, ankyrin family proteins play key roles in various processes including cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Ankyrin R is found expressed in erythrocytes, skeletal muscle and brain. Diseases associated with this gene include Spherocytosis.

Immunogen:

Fusion protein amino acids 1-1881 (full-length) of human Ankyrin-R (also known as Ankyrin-1, Erythrocyte Ankyrin, ANK-1, ANK and rCG_43073, accession number P16157) Mouse: 90% identity (1678/1858 amino acids identical)
Rat: 74% identity (1329/1788 amino acids identical)
50% identity with Ankyrin-B and Ankyrin-G

Cross Reactivity:

Does not cross-react with Ankyrin-B or Ankyrin-G

Protein Name(s):

Ankyrin-1 (ANK-1) (Ankyrin-R) (Erythrocyte ankyrin)

Gene Name(s):

ANK1 ANK

Antibody Registry ID:

Purified: AB_2491109
TC Supernatant: AB_2336901

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

200 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.