Amyloid beta, 1-42, human, oligomeric, Stabilized Peptide

Name: Amyloid beta, 1-42, human, oligomeric, Stabilized Peptide
Brand: Biosensis
SKU: PE-1750-1000
Price: 117.0 USD
Availability: InStock

Amyloid beta, 1-42, human, oligomeric, stabilized peptide for use as a stable and consistent standard or positive control for oligomeric ELISA assays.

Oligomeric A-beta standard curves generated with BEK-2215 (Oligomeric A beta ELISA kit). Pre-formed oligomers (PE-1750-1000) were reconstituted in assay buffer and compared to oligomers freshly prepared from HFIP-treated A-beta peptide (PE-1749-50). This data demonstrates the usefulness of PE-1750-1000 as oligomeric A-beta protein standard.

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Applications

ELISA

Host Species

Synthetic

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Data Sheet

SKU: PE-1750-1000

Volume/Size: 2 x 500 ng

Variant

Price:

$117.00

Quantity:

Product Details

Target

Amyloid beta, oligomeric

Format

Purified. Supplied as 2 x 500 ng vials, each containing lyophilized Aβ oligomers. Note that the amount of provided oligomeric protein is based on the amount of monomeric Aβ used to form these oligomers. The precise formation, size and number of oligomers cannot be quantified by any known method.

Applications

ELISA

Host Species

Synthetic

Protein Sequence

DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA

Storage

Store unopened, lyophilized oligomeric Aβ with desiccant, insulated, at -20°C short term, -80°C long term. Store reconstituted vial at 2-8°C for up to 2 days. The reconstituted material should not be frozen for best results.

Physical State

Lyophilized

Production Notes

Purified. A proprietary preparation of human amyloid beta peptide (amino acids 1-42) that was initially monomerized by HFIP-treatment and then allowed to form oligomers by the procedure described in Youmans KL et al., 2012, followed by lyophilisation using Biosensis' proprietary stabilization procedures.

The resulting oligomeric mixture has been specially designed to allow the formation of stable, oligomeric Aβ_1-42 peptide, multimeric complexes or oligomers.

Application Details

Use as positive control in Oligomeric Aβ ELISA Kit (BEK-2215): Reconstitute one vial with 1 mL of assay buffer provided in the ELISA kit. Dilute to a concentration of 0.5-1 ng/mL. At this concentration, a positive signal will be obtained within the dynamic range of the calibration curve.

Use as oligomeric A-beta peptide standard in Oligomeric Aβ ELISA Kit (BEK-2215): Reconstitute one vial with 1 mL of assay buffer provided in the ELISA kit. Dilute to a concentration of 2 ng/mL, which represents the highest concentration of the calibration curve. Perform a 1:2 serial dilution down to 0.031 ng/mL in assay buffer. Click here for detailed instructions on generating a calibration curve with PE-1750-1000.

Use as positive control in other applications: Optimal concentrations need to be determined empirically. It is recommended to reconstitute the vial with 100 - 200 µL buffer first (eg., PBS, pH 7.4), and prepare further working dilutions thereof.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

6 months after date of receipt (unopened vial).

Synonyms

Beta-APP42; Beta-amyloid protein 42; ABPP; APPI; Amyloid beta A4 protein; AB42; abeta

UniProt ID

P05067

Shipping

25°C (ambient)

A-Beta Peptide Instructions for Generating a Calibration Curve

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Amyloid beta, 1-42, human, oligomeric, Stabilized Peptide

SKU: PE-1750-1000

Product Details

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