Anti-NGFR/p75 neurotrophin receptor (p75NTR) Antibody (ATTO 488) (MC192)

Name: Anti-NGFR/p75 neurotrophin receptor (p75NTR) Antibody (ATTO 488)
Brand: Biosensis
SKU: M-026-50-AT
Price: 327.0 USD
Availability: InStock

Our Anti-NGFR/p75 neurotrophin receptor (p75NTR) mouse monoclonal primary antibody detects rat NGFR/p75 neurotrophin receptor (p75NTR), and is IgG. It is validated for use in FC, ICC, IHC-Frozen, IHC-Paraffin-embedded.

Photostability of ATTO 488 compared to common dyes in water. Excitation at 488nm with 1W Argon-Ion Laser.

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Species Reactivity

Rat

Applications

Flow, ICC, IHC

Host Species

Mouse

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Data Sheet

SKU: M-026-50-AT

Volume/Size: 50 µg

Variant

Price:

$327.00

Quantity:

Product Details

Target

NGFR/p75 neurotrophin receptor (p75NTR)

Scientific Background

Monoclonal antibody MC192 against the rat low affinity nerve growth factor receptor (p75NTR) is derived from the fusion of Sp2/0-Ag 14 myeloma cells with mouse immune splenocytes. MC192 monoclonal antibody was originally generated by Chandlers et al. p75NTR was originally discovered as a low affinity nerve growth factor receptor. Later it was found that it was the receptor for all neurotrophins. It mediates signals of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. Recently, it has been revealed that p75NTR not only acts as the receptor for neurotrophins but also the receptor for many other pathological ligands such as prions, rabies virus and amyloid beta. p75NTR also acts as a co-receptor for NOGO which mediates inhibitory signals of myelin associated protein. p75NTR is highly expressed in a number of non-neuronal and neuronal cells including motor neurons during development and also in damaged neurons. MC192 has a potential use as the ligand for gene delivery into p75NTR-expressing rat cells via a receptor-mediated mechanism. FUNCTION: Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with NGFRAP1/BEX3. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with SQSTM1 which bridges NGFR to NTRK1 (By similarity).

Format

IgG

Clonality

Monoclonal

Clone

MC192

Isotype

IgG1

Applications

Flow, ICC, IHC

Host Species

Mouse

Immunogen

Rat p75NTR

Immunogen Species

Rat

Species Reactivity

Rat

Storage

The antibody conjugate can be stored at 2-8°C for up to 4 months with the addition of appropriate antibacterial agent.

Physical State

Liquid

Production Notes

Immunoglobulin (IgG1) was purified using Protein G column (Amersham Pharmacia), polished with Sephacryl 200HR (Amersham Pharmacia) in PBS. The IgG was then conjugated to ATTO 488 (ATTO TEC) and purified via gel filtration using a G25 fine grain gel in 10 mMTris/50mM NaCl solution.

Dilution Ranges

IHC: 2-5 µg/mL
ICC: 2-5 µg/mL

Application Details

IF: live or lightly fixed cells or tissues (acetone or 4% PFA): 2-5 µg/mL. Not suitable for Western blots; not suitable for IHC on formalin fixed tissues. FACS (20 µg/mL) is recommended, unfixed cells.

Conjugate

ATTO 488 Ex: 500 nm, Em: 520 nm

Specificity

MC192 recognizes the extracellular domain of the neurotrophin receptor p75NTR in rat and does not react with human or mouse NGFR. Reacts with rat. Does not react with mouse or human NGFR.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

4 months after date of receipt (unopened vial)

Synonyms

Low-affinity nerve growth factor receptor; NGF receptor; Gp80-LNGFR; p75 ICD; Low affinity neurotrophin receptor p75NTR; CD271

UniProt ID

P07174

Shipping

2-8°C (on cold packs)

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Anti-NGFR/p75 neurotrophin receptor (p75NTR) Antibody (ATTO 488) (MC192)

SKU: M-026-50-AT

Product Details

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