Anti-Neurofilament heavy polypeptide, phosphorylated, (pNF-H) Antibody (NAP4) (NAP4)

Name: Anti-Neurofilament heavy polypeptide, phosphorylated, (pNF-H) Antibody (NAP4)
Brand: Biosensis
SKU: M-1387-50
Price: 327.0 USD
Availability: InStock

Our Anti-Neurofilament heavy polypeptide, phosphorylated (pNF-H) mouse monoclonal primary antibody detects bovine, chicken, horse, human, mouse, other mammals (predicted), pig, and rat Neurofilament heavy polypeptide, phosphorylated (pNF-H), and is IgG. It is validated for use in FC, IF, ICC, IHC, WB.

Image of a human cerebellar cortex section by Immunohistochemistry. The section was stained with M-1387-50, Neurofilament heavy polypeptide, phosphorylated (pNF-H), Clone NAP4, Mouse mAb (brown) and co-stained with heamatoxylin-eosin (blue). This antibody stains prominent basket cell axons surrounding the large Purkinje neurons. The granule cell layer is at the bottom of the image with the molecular layer at the top. IHC method: Section are fixed in formalin, embedded in paraffin using the ABC (avidin biotin conjugate).

Analysis by flow cytometry of Neurofilament heavy polypeptide, phosphorlylated (pNF-H), (endogenous) expression in mouse neural progenitor cells differentiated from mES and fixed overnight in 70% ethanol (Red curve). PE-labelled goat anti-mouse IgG was used as secondary antibody. Negative control processed with secondary antibody only (Blue curve). Flow cytometry data and results were generated using Orflo MoxiflowTM instrument and protocols.

Analysis by western blot of pNF-H expression in tissue lysates with M-1387-50, Neurofilament heavy polypeptide, phosphorylated (pNF-H), Clone NAP4, Mouse mAb, (green, 1:10,000) with a strong band at approx 200-220kDa corresponding to the phosphorylated form of the NF-H subunit. A minor band is observed at 160kDa corresponding to the non-phosphorylated NF-H. Smaller proteolytic fragments of NF-H are also detected in spinal cord preparations. Lane 1: MWM; Lane 2: rat brain; Lane 3: rat spinal cord; Lane 4: mouse brain; Lane 5: mouse spinal cord; Lane 6: pig spinal cord; Lane 7: cow spinal cord.

Image of a human cerebellar cortex section by Immunohistochemistry. The section was stained with M-1387-50, Neurofilament heavy polypeptide, phosphorylated (pNF-H), Clone NAP4, Mouse mAb (brown) and co-stained with heamatoxylin-eosin (blue). This antibody stains prominent basket cell axons surrounding the large Purkinje neurons. The granule cell layer is at the bottom of the image with the molecular layer at the top. IHC method: Section are fixed in formalin, embedded in paraffin using the ABC (avidin biotin conjugate).

Click on image to zoom

Species Reactivity

Bovine, Chicken, Horse, Human, Mouse, Pig, Rat

Applications

Flow, ICC, IF, IHC, WB

Host Species

Mouse

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Data Sheet

SKU: M-1387-50

Volume/Size: 50 µg

Variant

Price:

$327.00

Quantity:

Product Details

Target

Neurofilament heavy polypeptide , phosphorylated (pNF-H)

Scientific Background

Neurofilaments are the 10 nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H, though other filament proteins may be included also. The major function of neurofilaments is likely to control the diameter of large axons. NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at 68-70 kDa with some variability across species. Antibodies to NF-L are useful for identifying neuronal cells and their processes in cell culture and sectioned material. NF-L antibody can also be useful for the visualization of neurofilament rich accumulations seen in many neurological diseases, such as Lou Gehrig's disease (ALS), giant axon neuropathy, Charcot-Marie Tooth disease and others. (Ref: uniprot.org)

Format

IgG

Clonality

Monoclonal

Clone

NAP4

Isotype

IgG1

Applications

Flow, ICC, IF, IHC, WB

Host Species

Mouse

Immunogen

This antibody has been made against a native axonal phosphorylated NF-H purified from bovine spinal cord.

Immunogen Species

Bovine

Species Reactivity

Bovine, Chicken, Horse, Human, Mouse, Pig, Rat

Storage

Spin vial briefly before opening. Reconstitute with 50 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. Store lyophilized antibody at 2-8°C After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles. Store at 4°C for up to one month for short term storage and frequent use.

Physical State

Lyophilized

Production Notes

Protein G purified

Buffer

Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide

Dilution Ranges

WB: 1:10000
ICC: 1:1000

Application Details

This antibody recognizes NF-H in frozen sections, tissue culture and in formalin-fixed sections. A dilution of 2 ug/10^6 cells is recommended for FC.

Conjugate

Unconjugated

Specificity

Species cross-reactivity includes human, rat, mouse, cow, pig, horse and chicken. This antibody recognizes phosphorylated NF-H KSP (lysine-serine-proline) type sequences. In some species there is some cross-reactivity with the related KSP sequences found in subunit NF-M. Predicted to react with other mammals due to sequence homology.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

NF-H; NFH; NF-200; NF200; NF-H; NEFH; N52; Neurofilament heavy polypeptide; Neurofilament triplet H protein; 200 kDa neurofilament protein; KIAA0845

UniProt ID

F1MSQ6

Shipping

25°C (ambient)

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Anti-Neurofilament heavy polypeptide, phosphorylated, (pNF-H) Antibody (NAP4) (NAP4)

SKU: M-1387-50

Product Details

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