Anti-CamKII subunit alpha, Thr253 Antibody

Our Anti-Phospho-calcium/calmodulin-dependent protein kinase type II subunit alpha, Thr253 (alpha-CaMKII, Thr253) rabbit polyclonal primary antibody detects rat Phospho-calcium/calmodulin-dependent protein kinase type II subunit alpha, Thr253 (alpha-CaMKII, Thr253), and is whole serum. It is validated for use in WB.

Western blot analysis of recombinant FLAG-alpha-CaMKII purified from transfected COS cells. Blots of wild-type CaMKII phosphorylated at Thr253 were treated with lambda phosphatase prior to probing with anti-pT253 or anti-FLAG antibody. The pT253 antibody was incubated with phospho-Thr253 (pT253, 50 nM), non-phospho-Thr253 (T253, 500 nM) or phospho-Thr286 (pT286, 50 nM) peptides prior to probing the western blots. Reference: Migues PV et al (2006) J Neurochem 98 (1), 289-299.
Western blots of wild-type CaMKII showing auto-phosphorylation over 4 min at 10 µM ATP, 30°C and probed with antibodies to α-CaMKII, phospho-Thr286-CaMKII (anti-pT286) and phospho-Thr253-CaMKII (anti-pT253). The arrow-heads indicate the electrophoretic mobility of CaMKII phosphorylated at Thr253. Reference: Migues PV et al (2006) J Neurochem 98 (1), 289-299.
Western blot analysis of recombinant FLAG-alpha-CaMKII purified from transfected COS cells. Blots of wild-type CaMKII phosphorylated at Thr253 were treated with lambda phosphatase prior to probing with anti-pT253 or anti-FLAG antibody. The pT253 antibody was incubated with phospho-Thr253 (pT253, 50 nM), non-phospho-Thr253 (T253, 500 nM) or phospho-Thr286 (pT286, 50 nM) peptides prior to probing the western blots. Reference: Migues PV et al (2006) J Neurochem 98 (1), 289-299.
Western blot analysis of recombinant FLAG-alpha-CaMKII purified from transfected COS cells. Blots of wild-type CaMKII phosphorylated at Thr253 were treated with lambda phosphatase prior to probing with anti-pT253 or anti-FLAG antibody. The pT253 antibody was incubated with phospho-Thr253 (pT253, 50 nM), non-phospho-Thr253 (T253, 500 nM) or phospho-Thr286 (pT286, 50 nM) peptides prior to probing the western blots. Reference: Migues PV et al (2006) J Neurochem 98 (1), 289-299.
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Rat
WB
Rabbit

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SKU: R-1706-100

Volume/Size: 100 µL
Price:
$327.00

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