Monoclonal Antibodies

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Western blot analysis of adult mouse brain tissue lysate. The blot was probed with mouse monoclonal anti-PKCδ (N-terminal region) at 1:125 (lane 1) and 1:500 (lane 2).Immunocytochemical labeling of PKCδ in rat PC12 cells differentiated with NGF. The cells were labeled with mouse monoclonal PKCδ (N-terminal region) antibody, then detected using appropriate secondary antibody conjugated to Cy3.
PhosphoSolutions Anti-PKCδ (N-terminal region) Antibody
$370
Western blot analysis of PKC isoforms in adult mouse brain lysate. The blot was probed with mouse monoclonal anti-PKC (α,β,γ) clone M499 at 1:250 (lane 1) and 1:1000 (lane 2).
PhosphoSolutions Anti-PKC (α,β,γ) Antibody
$285
Western blot analysis of immunoprecipitates from neonatal rat brain lysate using anti-PKCα antibody. Control and alkaline phosphatase treated precipitates were probed with anti-PKCα (Central region) or anti-phospho-PKCα (Ser-657/Tyr-658). The latter shows no detection of PKCα after phosphatase treatment.Formalin fixed, citric acid treated parafin sections of adult mouse brain. Sections were probed with anti-PKCα (PM2371) then anti-mouse:HRP before detection using DAB. (Image provided by Carl Hobbs and Dr. Pat Doherty at Wolfson Centre for Age-Related Diseases, King's College London).
PhosphoSolutions Anti-PKCα (Central region) Antibody
$370
Western blot image of human A431 cells. The blots were probed with mouse monoclonal anti-PI3 Kinase, p85 (C-terminal region) at 1:1000 (lane 1), 1:2000 (lane 2), and 1:4000 (lanes 3).Immunocytochemical labeling of PI3 Kinase p85 in aldehyde-fixed and NP-40-permeabilized A431 cells. The cells were labeled with mouse monoclonal PI3 Kinase p85 (C-terminal region) antibody (PM2531), then the antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.
PhosphoSolutions Anti-PI3 Kinase, p85 (C-terminal region) Antibody
$370
Western blot of HeLa cells treated with pervanadate (1 mM) for 30 min. Phosphotyrosine containing proteins were immunoprecipitated with rabbit polyclonal anti-Phosphotyrosine:Agarose (Lane 1) or with no antibody agarose beads (Lane 2), and blots were made that included the whole lysate (Lane 3). The blots were probed with mouse monoclonal anti-Phosphotyrosine (PM3751) to detect phosphotyrosine containing proteins.Immunocytochemical labeling of phosphotyrosine in control and pervanadate-treated A431 cells. The cells were labeled with rabbit polyclonal anti-Phosphotyrosine (PP2221) and mouse monoclonal anti-Phosphotyrosine (PM3751), then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.
PhosphoSolutions Anti-Phosphotyrosine Antibody
$285
Western blot of human recombinant PD-L1 extracellular region (40 kDa; lanes 1 & 3) and endogenous PD-L1 in human lung (lanes 2 & 4). The blot was probed with mouse monoclonals anti-PD-L1 (PM0511) at 1:500 (lanes 1 & 2) and anti-PD-L1 (PM0801) at 1:250 (lanes 3 & 4).Immunocytochemical labeling of PD-L1 in aldehyde fixed human MDA-MB-231 carcinoma cells untreated (A) or treated with 100 ng/ml IFNγ for 48 hrs (B). The cells were labeled with mouse monoclonal anti-PD-L1 (PM0801). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-PD-L1 (Extracellular region) M080 Antibody
$370
Western blot of human recombinant PD-L1 extracellular region (40 kDa; lanes 1 & 3) and endogenous PD-L1 in human lung (lanes 2 & 4). The blot was probed with mouse monoclonals anti-PD-L1 (PM0511) at 1:500 (lanes 1 & 2) and anti-PD-L1 (PM0801) at 1:250 (lanes 3 & 4).Immunocytochemical labeling of PD-L1 in aldehyde fixed human MDA-MB-231 carcinoma cells untreated (A) or treated with 100 ng/ml IFNγ for 48 hrs (B). The cells were labeled with mouse monoclonal anti-PD-L1 (PM0511). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-PD-L1 (Extracellular region) M051 Antibody
$370
Western blot image of mouse brain (lane 1) and human A431 cells (lane 2). The blot was probed with anti-PDK1 (C-terminal region) (PM1461).Immunocytochemical labeling of PDK1 phosphorylation in control and pervanadate-treated A431 cells. The cells were labeled with mouse monoclonal PDK1 (C-terminal Region) (PM1461), rabbit polyclonal PDK1 (Tyr-9) (PP1431), and PDK1 (N-terminus) (PP1411) antibodies. These antibodies were detected using appropriate secondary antibody conjugated to DyLight® 594.
PhosphoSolutions Anti-PDK1 (C-terminal region) Antibody
$285
Western blot analysis of A431 cells (30 µg/lane) serum starved overnight (lane 1 & 3) or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). The blot was probed with anti-Paxillin (PM1071) or anti-phospho-Paxillin (Tyr-31) (PM1021).
PhosphoSolutions Anti-Paxillin (Tyr-31), Phosphospecific Antibody
$340
Western blot analysis of A431 cells (20 µg/lane) serum starved overnight and treated with EGF (100 ng/ml) for 5 min. The blot was probed with anti-Paxillin mouse monoclonal (PM1071) or anti-Paxillin (Ser-178) rabbit polyclonal (PP1051).Immunocytochemical labeling of Ser-83 phosphorylated paxillin in rabbit spleen fibroblasts. The cells were labeled with mouse monoclonal Paxillin (left) and rabbit polyclonal Paxillin (Ser-83, right) antibodies, then detected using appropriate secondary antibodies conjugated to Cy3.
PhosphoSolutions Anti-Paxillin Antibody
$370
Western blot analysis of human endothelial cells serum starved overnight (lanes 1, 3, & 5) or treated with pervanadate (1 mM) for 30 minutes (lanes 2, 4, & 6). The blot was probed with anti-p130 Cas (PM1441; lanes 1 & 2), anti-p130 Cas (Tyr-751) (PP1581; lanes 3 & 4) or anti-p130 Cas (Tyr-762) (PP1451; lanes 5 & 6).Immunocytochemical labeling of p130 Cas in aldehyde fixed human A431 epidermoid carcinoma cells. The cells were labeled with mouse monoclonal anti-p130 Cas (PM1441). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-p130 Cas (C-terminal region) Antibody
$370
Western blot analysis of A431 cells serum starved overnight (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 minutes (lanes 2 & 4). The blot was probed with anti-p38α (lanes 1 & 2) or anti-p38 (T180/Y182) (lanes 3-4). Lanes 5-7 shows a blot of A431 cells treated with pervanadate and probed with anti-p38 (T180/Y182) in the presence of no peptide (lane 5), phospho-ERK1 (T202/Y204) peptide (lane 6) or phospho-p38 (T180/Y182) peptide (lane 7).Immunocytochemical labeling of activated p38 MAPK in pervanadate-treated mouse C2C12. The cells were labeled with mouse monoclonal p38α MAPK and p38 MAPK (T180/Y182) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.
PhosphoSolutions Anti-p38 MAP Kinase (Thr-180/Tyr-182), Phosphospecific Antibody
$370
Western blot analysis of human A431 cells (lanes 1 & 2) and rat A7r5 cells (lanes 3 & 4). The blot was probed with mouse monoclonal anti-p38α (C-terminal) clone M548 at a dilution of 1:250 (lanes 1 & 3) or 1:1000 (lanes 2 & 4).Immunocytochemical labeling of p38α in aldehyde fixed and NP-40 permeabilized human NCI-H446 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-p38α (PM5481). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-p38α MAP Kinase (C-terminal) Antibody
$285
Western blot analysis of A431 cells serum starved overnight (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 minutes (lanes 2 & 4). The blot was probed with anti-p38α (lanes 1 & 2) or anti-p38 (T180/Y182) (lanes 3-4). Lanes 5-7 shows a blot of A431 cells treated with pervanadate and probed with anti-p38 (T180/Y182) in the presence of no peptide (lane 5), phospho-ERK1 (T202/Y204) peptide (lane 6) or phospho-p38 (T180/Y182) peptide (lane 7).Immunocytochemical labeling of activated p38 MAPK in pervanadate-treated mouse C2C12. The cells were labeled with mouse monoclonal p38α MAPK and p38 MAPK (T180/Y182) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.
PhosphoSolutions Anti-p38α MAP Kinase (C-terminal) M138 Antibody
$370
Western blot analysis of p21 expression in human endothelial cells (lanes 1 & 2). The blots were probed with mouse monoclonal anti-p21 at 1:250 (lane 1) and 1:1000 (lane 2).
PhosphoSolutions Anti-p21/CIP1/WAF1 Antibody
$285
Western blot analysis of human recombinant osteopontin protein (lane 1 & 2) or human MDA-MB-231 cells (lane 3 & 4). The blots were probed with mouse monoclonal anti-Osteopontin (OM5741) at a dilution of 1:1000 (lane 1), 1:4000 (lane 2), 1:250 (lane 3), and 1:1000 (lane 4).Immunocytochemical labeling of Osteopontin in paraformaldehyde fixed and NP-40 permeabilized MCF-7 cells. The cells were labeled with mouse monoclonal anti-Osteopontin (M574). The antibody was detected using goat anti-mouse DyLight® 594.
PhosphoSolutions Anti-Osteopontin (N-terminal region) Antibody
$340
Immunocytochemical labeling of Nucleoporin p62 in paraformaldehyde-fixed and NP40-permeabilized A7r5 cells. The fixed cells were labeled with mouse monoclonal anti-Nucleoporin p62 (N-terminal region) and the antibody was detected using Goat anti-Mouse secondary antibodies conjugated to DyLight® 488.Western blot image of cell structure markers in NCI-H1915 lung carcinoma cells. The blot was probed with anti-Vimentin intermediate filament protein VM4341 (lane 1), anti-Nucleoporin p62 NM4361 (lane 2), anti-Hsp60 mitochondrial protein HM4381 (lane 3), and anti-Calnexin endoplasmic reticulum protein CM4371 (lane 4).
PhosphoSolutions Anti-Nucleoporin p62 (N-terminal region) Antibody
$370
Western blot analysis of nNOS expression in adult mouse brain (lanes 1 & 3) and rat GC cells (lanes 2 & 4). The blots were probed with mouse monoclonal anti-nNOS (C-terminal region) at 1:1000 (lanes 1 & 2) or rabbit polyclonal anti-nNOS at 1:250 (lanes 3 & 4).Immunocytochemical labeling of nNOS phosphorylation in rat PC12 cells differentiated with NGF. The cells were probed with mouse monoclonal (mAb) nNOS (NM4011), and rabbit polyclonal (pAb) nNOS (C-terminal region), nNOS (Tyr-895)/eNOS (Tyr-657), and nNOS (Tyr-1326)/iNOS (Tyr-1055). The antibodies were detected using appropriate secondary antibody conjugated to DyLight® 594.
PhosphoSolutions Anti-nNOS (C-terminal region) Antibody
$370
Western blot analysis of mouse macrophages untreated (lanes 1 & 3) or treated with LPS (1µg/ml) for 18 hrs (lanes 2 & 4). The blots were probed with mouse monoclonal anti-iNOS at 1:500 (lanes 1 & 2) or rabbit polyclonal anti-iNOS at 1:250 (NP2131).
PhosphoSolutions Anti-iNOS (C-terminal region) Antibody
$285
Western blot analysis of human umbilical vein endothelial cells stimulated with Calyculin A and untreated (lanes 1, 3, 5) or treated (lanes 2, 4, 6) with lambda phosphatase. The blots were probed with anti-endothelial Nitric Oxide Synthase (eNOS) monoclonal antibody (lanes 1 & 2), anti-eNOS (Ser-632) phospho-specific antibody (lanes 3 & 4), and anti-eNOS polyclonal antibody (lanes 5 & 6).
PhosphoSolutions Anti-eNOS (Ser-632), Phosphospecific Antibody
$340
Western blot analysis of human umbilical vein endothelial cells before (lanes 1, 3, 5) and after (lanes 2, 4, 6) treatment with lambda phosphatase. The blots were probed with anti-endothelial Nitric Oxide Synthase (eNOS) monoclonal antibody (lanes 1 & 2), anti-eNOS (Ser-632) phospho-specific antibody (lanes 3 & 4), and anti-eNOS polyclonal antibody (lanes 5 & 6).Immunocytochemical labeling of endothelial nitric oxide synthase (eNOS) in paraformaldehyde-fixed and NP-40-permeabilized human umbilical vein endothelial cells. The cells were labeled with mouse monoclonal eNOS (NM2211) and rabbit polyclonal eNOS (NP2281) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.
PhosphoSolutions Anti-eNOS (C-terminal region) Antibody
$370
Western blot image of mouse recombinant NLRC4 (Ser-533) phosphorylated peptide (lanes 1-4). The blot was dephosphorylated with lambda phosphatase (lanes 2 & 4) then the blot was probed with rat monoclonal anti-NLRC4 (Ser-533) phospho-specific (lanes 1 & 2) and rabbit polyclonal anti-NLRC4 (a.a. 525-538) (lanes 3 & 4).
PhosphoSolutions Anti-NLRC4 (Ser-533), Phosphospecific Antibody
$340
Western blot image of recombinant human Neuropilin-1 (lanes 1 & 3) and human PC3 cells (lanes 2 & 4). The blots were probed with rabbit polyclonal anti-Neuropilin-1 (NP2111) (lanes 1 & 2) or with mouse monoclonal anti-Neuropilin-1 (lanes 3 & 4).
PhosphoSolutions Anti-Neuropilin-1 (a1 CUB Domain) Antibody
$340
Western blot of native human MDA-MB-231 (lane 1), and MeWo (lane 2) cell lysates, as well as native human breast (lane 3), lung (lane 4), skin (lane 5), and brain (lane 6) tissues. The blot was probed with anti-Na+/K+ ATPase β3 (NM0251) at 1:1000.Immunocytochemical labeling of Na+/K+ ATPase β3 in paraformaldehyde fixed human MeWo melanoma cells. The cells were labeled with mouse monoclonal anti-Na+/K+ ATPase β3 (clone M025). The antibody was detected using goat anti-mouse Ig DyLight® 594.
PhosphoSolutions Anti-Na+/K+ ATPase β3 (Extracellular) Antibody
$370

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