Monoclonal Antibodies

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Immunocytochemical labeling of Vimentin in paraformaldehyde fixed and NP-40 permeabilized A7r5 cells. The cells were labeled with a mouse monoclonal antibody to Vimentin (VM4341), then the antibody was detected using Goat anti-Mouse secondary antibody conjugated to DyLight® 594.Western blot image of cell structure markers in NCI-H1915 lung carcinoma cells. The blot was probed with anti-Vimentin intermediate
filament protein VM4341 (lane 1), anti-Nucleoporin p62 NM4361 (lane 2), anti-Hsp60 mitochondrial protein HM4381 (lane 3), and anti-Calnexin endoplasmic reticulum protein CM4371 (lane 4).
Western blot image of human A431 cells stimulated with calyculin A (100 nM) for 30 min. The blots were untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4), then probed with mouse monoclonal VASP (C-term.) antibody (lanes 1 & 2) or rabbit polyclonal VASP (Thr-278) phospho-specific antibody (lanes 3 & 4).Immunocytochemical labeling of VASP in aldehyde-fixed and NP-40-permeabilized A431 cells. The cells were labeled with mouse monoclonal VASP (C-terminal region) antibody, then the antibody was detected using appropriate secondary antibody conjugated to DyLight® 594.
Western blot analysis of purified brain tubulin untreated (lanes 1,3,5) or treated with ERK2 kinase to phosphorylate Ser-172 (lanes 2,4,6). The blot was probed with anti-β-Tubulin (a.a. 168-177) (lanes 1 & 2), anti-β-Tubulin (Ser-172) (lanes 3 & 4), and anti-β-Tubulin (TM1541) (lanes 5 & 6).Immunocytochemical labeling in C2C12 cells using anti-β-Tubulin (TM1541) monoclonal antibody and anti-β-Tubulin (Ser-172) polyclonal antibody. The specificity of the binding for the latter antibody was demonstrated by using the antibody in the presence of phospho-β-Tubulin (Ser-172) peptide (TX1725).
Western blot analysis of α-tubulin expression in human A431 (lane 1), HUVEC (lane 2), Jurkat (lane 3), mouse J774.1 (lane 4), human PC-3 (lane 5), rat PC12 (lane 6), and mouse C2C12 (lane 7). The blot was probed with anti-α-Tubulin (C-terminus) at 1:1000.Immunocytochemical labeling of α- and βI-Tubulin in rat A7r5 cells. The cells were labeled with anti-βI-Tubulin (TM1541) (left) and anti-α-tubulin (TM4111) (right). The antibodies were detected using Goat anti-Mouse conjugated to DyLight® 488.
Western blot of rat PC12 cells stimulated with Calyculin A (100 nM) for 30 min (lanes 1-4). The blot was treated with lambda phosphatase (lanes 2 & 4), then probed with mouse monoclonal anti-Talin (8D4) (lanes 1 & 2) or rabbit polyclonal anti-Talin (Ser-425) (lanes 3 & 4) antibodies.Immunocytochemical labeling of Talin relative to F-actin in chick fibroblasts. The cells were labeled with mouse monoclonal Talin (Rod domain) antibody (TM4081), then the antibody was detected using appropriate secondary antibody (Red). This labeling is compared to F-actin staining (Green). (Image provided by Dr. Gianluca Gallo at Drexel University).
Western blot of adult mouse spleen lysate. The blot was probed with mouse monoclonal anti-Syk (Central region) antibody at 1:250.
Western blot analysis of human A431 cells untreated (lanes 1, 3, 5, 7, & 9) or treated with EGF (100 ng/ml for 60 min (lanes 2, 4, 6, 8, & 10). The blots were probed with anti-Stat1 (lanes 1 & 2), anti-Stat1 (Tyr-701) (lanes 3 & 4), anti-Stat3 (lanes 5 & 6), anti-Stat5 (lanes 7 & 8), and anti-Stat5 (Tyr-694) (lanes 9 & 10).Immunocytochemical labeling of Stat5 in control and pervanadate-treated A431 cells. The cells were labeled with mouse monoclonal Stat5 (SM2511) or Stat5 (Tyr-694) (SM1481) antibodies, then the antibodies were detected using appropriate secondary antibody conjugated to DyLight® 594.
Western blot analysis of human A431 cells untreated (lanes 1, 3, 5, 7, & 9) or treated with EGF (100 nM) for 60 min (lanes 2, 4, 6, 8, & 10). The blots were probed with anti-Stat1 (lanes 1 & 2), anti-Stat1 (Tyr-701) (lanes 3 & 4), anti-Stat3 (lanes 5 & 6), anti-Stat5 (lanes 7 & 8), and anti-Stat5 (Tyr-694) (lanes 9 & 10).

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