Diaminobezidine (DAB) Kit
Peroxidase chromogen-substrate 3-3´ diaminobezidine (DAB) reagent is an excellent chromogen system that is used in Immunohistochemistry (IHC) and Immunocytochemistry (ICC).
Diaminobezidine (DAB) Kit
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Ships: 1-2 business days
Mixing of reagent:
- To one ml of Buffer Substrate (BS) in a test tube, add two drop of Chromogen (C) reagent mix well.
- This ready-to-use reagent is good for several hours. The unused DAB solution can be discarded according to city, county, state, province or country’s regulations.
- After the peroxidase reaction, wash slide with buffer (this buffer should not contain any sodium azide because peroxidase is inactivated) and distilled/ deionized water.
- Add few drops of ready to use DAB reagent on tissue slides for 5-10 minutes at RT. (Higher temperature 37°C can also be used for DAB chromogen, however the incubation time should be determined by the individual lab).
- Wash 2-5X with buffer, followed by rinsing with distilled/deionized water.
- Add counterstain compatible with DAB, we use hematoxylin 30-60 seconds (cat. # AR-6521) or any counterstain.
- Wash with tap water, buffer pH 7.4 or over, followed by rinsing with distilled/deionized water.
- Mount slide with aqueous mounting medium or organic mounting medium, after dehydration as described in the protocol for mounting medium.