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Immunoblot against extracts of COS cells transfected with tagged Beta- spectrins or untagged Kv2.1 plasmid probed with N393/2 (left) or 19E10 (right) TC supe.Adult rat brain membrane immunoblot.
Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged Synaptotagmin or untagged Kv2.1 plasmids and probed with N275/14 (left) and N86/8 (right).Adult rat brain membrane immunoblot.
Immunoblot against crude brain membrane preparations from adult rat (RBM) or extracts of COS cells transiently transfected with Myc- tagged LRRTM1, LRRTM2 or untagged Kv2.1 plasmid probed with N209C/35 (left), K89/34 (middle) or N52A/42 (right) TC supe.Adult rat brain immunohistochemistry.
Adult rat brain immunohistochemistry.Adult rat brain membrane immunoblot.
Transfected cell immunoblot: extracts of COS cells transiently transfected with Flag-tagged Olig1, Olig2 and untagged Kv2.1 plasmids and probed with N149/25 TC supe (left) and mouse anti-Flag (right.Immunofluorescence of postnatal day 17 mouse corpus callosum (CC) near lateral ventricle (LV). Data courtesy of Hui-Hsin Tsai and David Rowitch, UCSF.
Immunoblot versus lysates of total brain from HCN3 knockout (KO) and wild-type (WT) mice probed with N141/28. Data courtesy of Martin Biel (Ludwig-Maximilians Universität München) and reproduced from the Journal of Biological Chemistry (2013 Cao-Ehlker et al, PMID 23382386).Adult rat hippocampus immunohistochemistry.
Immunoblot versus rat brain low-speed pellet (LSP), mouse brain membranes (MBM) and wild-type (WT) and LRRK2 KO mouse samples probed with N138/6 (left) and K89/41 (right) TC supe. Mouse brain samples provided by Xiaojie Li, Ted Dawson and Valina Dawson (Johns Hopkins University).
Adult rat hippocampus immunohistochemistry.Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS cells transiently transfected with HA-tagged Kir2.1 or untagged Kv2.1 plasmids and probed with N112B/14 (left) and K89/41 (right) TC supe.
Adult rat hippocampus immunohistochemistry.Immunoblot against crude brain membranes from adult rat (RBM) and wild-type (WT) and Kv4.2 knockout (KO) mice probed with L28/4 (left) or N52A/42 (right) TC supe.
array tomography immunofluorescence of an LRWhite-embedded 70 nm section from adult mouse cortex with L106/4 (green), rabbit mAb GAD2 (Cell Signaling 5843, magenta) and DAPI (blue). The insert shows three consecutive sections through the synapse that is marked with a white box. Image courtesy of Kristina Micheva (Stanford). Adult rat hippocampus immunohistochemistry.
Adult rat hippocampus immunohistochemistryAnti-SAP97 Antibody
Adult rat brain immunohistochemistry.Adult rat brain membrane immunoblot.
Surface immunofluorescence (no detergent permeabilization) of cultured rat hippocampal neuron transfected with HA-tagged Kv2.1 plasmid. Red = K39/25. Scale bar = 20 m. Reproduced from 2000 Lim et al Neuron (PMID 10719893).Adult rat brain membrane immunoblot
Transfected cell immunoblot: COS cells transiently transfected with Ataxin-1 and Kv2.1 plasmids and probed with N76/8 TC supe.Adult rat brain immunoblot: extracts of cerebella from Ataxin-1 KO and WT mice and probed with N76/8 (left) or K89/41 (right) TC supe.
Adult rat hippocampus immunohistochemistry.Immunoblot against adult rat brain membranes (RBM) and extracts of COS cells transiently transfected with untagged KChIP1, KChIP2, KChIP3, KChIP4 or Kv2.1 plasmid probed with N423/75 (left) or K55/82 (right) TC supe.
Adult rat brain immunohistochemistry.Immunoblot against extracts of COS cells transiently transfected with GFP-tagged MAP3K12, MAP3K13 or untagged Kv2.1 plasmid; adult rat brain low-speed pellet (RBLSP) or membrane (RBM) fractions; and membranes from MAP3K12 wild-type (WT) and gene- trap knockout (KO) mice probed with N377/20 (left) or N52A/42 (right) TC supe. Mouse brains courtesy of Aki Itoh and Takayuki Itoh (UC Davis).
Immunofluorescence staining of adult wild-type (WT) and Kv2.2 knockout (KO) mouse magnocellular preoptic nucleus (MCPO) with N372B/1 (red) and Hoechst nuclear stain (blue).Adult rat whole brain immunohistochemistry.
Immunoblot against extracts of COS cells transiently transfected with Myc-tagged Kir6.1, HA- tagged Kir6.2 or untagged Kv2.1 plasmid probed with N366/60 (left) or N52A/42 (right) TC supe.Adult rat brain membrane immunoblot.
Immunoblot against crude brain membrane preparations from adult rat (RBM) or wild-type (WT) or KA2 knockout (KO) mice probed with N279B/27 (left) or N52A/42 (right) TC supe. Mouse brains courtesy of Anis Contractor (Northwestern University.
Adult rat brain membrane (RBM) and transfected cell immunoblot: extracts of RBM and COS-1 cells transiently transfected with KCNQ1, KCNQ2, KCNQ3, KCNQ4, KCNQ5 or Kv2.1 plasmids and probed with pure N26A/23.
Immunofluorescence of COS cells expressing 6xHis/Myc-tagged human VDAC1. Green = N144/14, red = Rabbit anti-Myc, blue = Hoechst nuclear stain.Immunoblot against extracts of COS cells transiently transfected with 6xHis/Myc-tagged Olig2 or untagged Kv2.1 plasmid probed with N144/14 (left), 19E10 (middle) or K89/34 (right) TC supe.
Adult rat brain membrane immunoblot.Lysates of rat cortical neurons treated with the indicated compounds were subjected to ABE to purify palmitoylated proteins. Levels of palmitoyl-AnkG-190 (right blot) and total AnkG (Cat. 75-187) expression in parent lysates (left blot bottom blot) were detected with specific antibodies. Exclusion of NH2OH was used as a control for assay specificity. Image from publication CC-BY-4.0. PMID:36969554
Immunofluorescence staining of adult rat optic nerve with K73/20 (red) and βIV-spectrin rabbit polyclonal (green). Image courtesy of Matt Rasband (Baylor College of Medicine).Adult rat brain membrane immunoblot.

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