Fluo-4, AM Ester Fluorescent Calcium Indicator

Name: Fluo-4, AM Ester Fluorescent Calcium Indicator
Brand: Antibodies Incorporated
SKU: 81-3006
Price: 339.0 USD
Availability: InStock

Our Fluo-4, AM Ester is a non-ratiometric calcium indicator that increases in fluorescence intensity upon calcium binding. Calcium imaging is widely used to measure neuronal activity.

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Applications

EM, ICC, IHC

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Data Sheet

SKU: 81-3006

Size: 10 x 50 µg

Variant

Price:

$339.00

Quantity:

Ships: 1-5 business days

Product Details

Scientific Background

To study the roles of calcium, a key intracellular messenger in cells, it is essential to quantitatively monitor its concentration. The most widely used method of Ca2+ detection is the use of fluorescent Ca2+ indicators. Fluo-4 is an analog of Fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm that gives higher fluorescence signal. Fluo-4 has its absorption maximum at 494 nm, thus making it excitable by the argon-ion laser. Fluo-4 is essentially nonfluorescent without Ca2+ present, but the fluorescence increases at least 100 times on Ca2+ binding. Also, because Fluo-4 binds Ca2+ more weakly (higher Kd) than do fura-2 and indo-1, it is more useful for measuring high transient Ca2+ concentration during Ca2+ spikes. Fluo-4, AM ester is a membrane-permeant form of Fluo-4 calcium indicator that can enter into into cells via incubation.

Applications

EM, ICC, IHC

Molecular Weight

1096.95 Da

Excitation / Emission

Kd: 335 nM, Ex: 494 nm, Em: 516 nm

Storage

Store at –20°C, with desiccant; protect from light.

Physical State

Solid

Specificity

Membrane-permeant, non-ratiometric; can be loaded into cells via incubation.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

Stable for 12 months after date of receipt.

Shipping

Shipped at ambient temperature with desiccant; protected from light

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Fluo-4, AM Ester Fluorescent Calcium Indicator

SKU: 81-3006

Product Details

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