Anti-GABA-A-R-Alpha2 Antibody (N399/19)

Name: Anti-GABA-A-R-Alpha2 Antibody
Brand: NeuroMab
SKU: 75-384
Price: 329.0 USD
Availability: InStock

5 Citations

Our Anti-GABA-A-R-Alpha2 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N399/19. It is KO validated, detects mouse and rat GABA-A-R-Alpha2, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Adult rat hippocampus immunohistochemistry.

Immunoblot against crude membranes from adult rat brain (RBM) and wild-type (WT) and GABA-A-R-Alpha2 knockout (KO) mouse brains probed with N399/19 (left) and N52A/42 (right) TC supe. Mouse brains courtesy of Uwe Rudolph (Harvard Medical School.

Whole brain immunohistochemistry.

Click on image to zoom

Species Reactivity

Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Carrier-Free

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Data Sheet

KO Validated

SKU: 75-384

Volume: 100 µL

Variant

Price:

$329.00

Quantity:

Ships: 1-2 business days

Product Details

Target

GABA-A-R-Alpha2

Scientific Background

Gamma-aminobutyric acid receptor subunit alpha-2 or GABA AR-Alpha2 is encoded by the gene GABRA2. GABAA receptors are members of the Cys-loop family of ligand-gated ion channels. GABA AR alpha 5 is a subunit of the pentameric GABA receptors where GABA is the major inhibitory neurotransmitter in the vertebrate brain and mediates neuronal inhibition by binding to the GABA receptor and opening an integral chloride channel. GABA AR-Alpha2 is primarily expressed in brain at the cell junction, synapse,and the postsynaptic cell membrane. Diseases associated with GABRA2 include Alcohol Dependence and Alcohol Use Disorder.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N399/19

Isotype

IgG1

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

Gabra2 Gabra-2

Molecular Weight

55 kDa

Immunogen

Fusion protein amino acids 350-385 (cytoplasmic C-terminus) of rat GABA-A-R-Alpha2 (accession number P23576) produced recombinantly in E. Coli

Immunogen Species

Rat

Species Reactivity

Mouse, Rat

Antibody Registry ID

AB_2686910

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Conjugate

Unconjugated

Specificity

Does not cross-react with GABA-A-R-Alpha1

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Gamma-aminobutyric acid receptor subunit alpha-2 (GABA(A) receptor subunit alpha-2)

UniProt ID

P23576

Shipping

Shipped on ice packs

Product Specific References for Applications and Species

Immunocytochemistry: Rat
Immunohistochemistry: Mouse | Rat
Western Blot: Mouse | Rat

Immunocytochemistry: Rat
PMID	Dilution	Publication
35312684	1:250	Schmerl, B., et al. 2022. The synaptic scaffold protein MPP2 interacts with GABAA receptors at the periphery of the postsynaptic density of glutamatergic synapses. PLoS Biology, e3001503.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
26435106	not listed	Sigal, Y.M., et al. 2015. Mapping Synaptic Input Fields of Neurons with Super-Resolution Imaging. Cell, 493-505.

Immunohistochemistry: Rat
PMID	Dilution	Publication
31642070	1:5 (supe)	Mueller, P.J., et al. 2020. Subregional differences in GABAA receptor subunit expression in the rostral ventrolateral medulla of sedentary versus physically active rats. . Journal of Comparative Neurology, 1053-1075.

Western Blot: Mouse
PMID	Dilution	Publication
39388350	1:1000	Dunham, TL, et al. 2024. WWC2 modulates GABAA-receptor-mediated synaptic transmission, revealing class-specific mechanisms of synapse regulation by WWC family proteins. Cell Reports, 114841.

Western Blot: Rat
PMID	Dilution	Publication
31642070	1:205 (supe)	Mueller, P.J., et al. 2020. Subregional differences in GABAA receptor subunit expression in the rostral ventrolateral medulla of sedentary versus physically active rats. . Journal of Comparative Neurology, 1053-1075.

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Anti-GABA-A-R-Alpha2 Antibody (N399/19)

SKU: 75-384

Product Details

Product Specific References for Applications and Species

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