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Methotrexate, CF®488A Conjugate
Methotrexate, CF®568 Conjugate
Methotrexate, CF®594 Conjugate
Methotrexate, CF®640R Conjugate
Mouse mNGF/proNGF Combo Rapid ELISA AssayMouse mNGF/proNGF Combo Rapid ELISA Assay
Mouse Serum
Figure 1. Assay principle: a non‐fluorescent detection reagent is oxidized in the presence of hydrogen peroxide and MPO to produce its fluorescent analog.Figure 2. A MPO standard curve was prepared and run, as described in the protocol, in the absence (A) or presence (B) of 20 mM catalase inhibitor. MPO standard and reaction mix were added to a 96-well black plate and incubated at room temperature in the dark for 30 minutes. The wells were read using Ex: 530nm and Em: 590nm. There is an approximately 50% reduction in signal in the presence of 20mM catalase inhibitor (note the different scales on the x-axis).

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