High-Sensitivity Detection for Immunofluorescence Applications!
We're excited to announce the launch of our Streptavidin/Biotin-ABC-Peroxidase Staining Kit (IH-8106-01) - a detection system that amplifies the sensitivity of your immunohistochemistry, immunocytochemistry, Western blot, and ELISA experiments. It is perfect for visualizing low-abundance proteins in tissue sections and highlighting cellular markers.
What Makes This Kit Special?
Unlike basic detection systems that provide one enzyme per antibody, our ABC (Avidin-Biotin Complex) amplification method creates a cascade of signal-generating enzymes at each detection site. This results in 10-20 times stronger signals compared to direct and traditional indirect detection methods.
Every component in this kit—including the Streptavidin-HRP conjugate—is manufactured in-house at our Davis, California facility. This gives us control over product quality and performance and ensures lot-to-lot consistency.
Included in our ABC Kit
This kit contains the essential detection components:
• Reagent A: 50X concentrated Streptavidin-HRP conjugate (2 mL)
• Reagent B: 50X concentrated Mixing reagent (4 mL)
Please note: This kit provides the amplification detection system. You'll need to supply your own primary antibodies, biotinylated secondary antibodies, chromogen substrates, blocking solutions, and mounting media. This design gives you maximum flexibility to use the antibodies optimized for your specific research targets combined with our superior detection technology.
How ABC Amplification Works
Step 1: Target Binding — Your primary antibody recognizes and binds to the target protein in your tissue or cells.
Step 2: Biotin Labeling — A biotinylated secondary antibody binds to your primary antibody. Each secondary antibody carries multiple biotin tags (typically 3-6 per molecule).
Step 3: Amplification — This is where you can use our kit to maximize your signal! Mix the A and B reagents in the assay buffer (without sodium azide) according to the protocol on the product page and incubate for 10-20 minutes with the samples previously incubated with the biotinylated secondary antibody. Multiple Avidin-Biotin-Complexes (ABCs) are formed by incubating our Streptavidin-HRP conjugate (Reagent A) in our proprietary mixing solution (Reagent B) with the biotinylated secondary antibody. Each streptavidin molecule can bind multiple biotin tags on the secondary antibody, and each carries HRP (horseradish peroxidase) enzymes. This creates a cluster of 10-20+ enzyme molecules at each antibody binding site.
Step 4: Visualization — When you add chromogen substrate, the HRP enzymes convert the substrate into an intense, visible colored precipitate. More enzymes mean more color—dramatically stronger signals that are easy to see.
Advantages of our ABC Kit for Your Research
Detect Low-Abundance Targets: Proteins that exist in small amounts in tissue can be hard to see without amplification. Our ABC method reveals targets that would be invisible with direct or traditional indirect detection.
Lower Background: Stronger signals mean better contrast between positive and negative cells. This makes data interpretation easier and provides publication-quality images with minimal optimization.
Flexibility Across Applications: While designed for IHC and ICC, this kit also performs excellently in Western blotting and ELISA. Pair it with any biotinylated antibody of your choice—the same detection system works across all your experiments.
High-Throughput Capability: With sufficient reagent for up to 1,000 tissue sections, this kit is ideal for large-scale screening projects, clinical research studies, or labs running multiple experiments simultaneously.
Complete Quality Control: Because we manufacture every component in-house, we test and validate each batch before it ships. You get consistent, reproducible results you can trust.
Get started today!
Ready to amplify your detection sensitivity? Our Streptavidin/Biotin-ABC-Peroxidase Staining Kit (IH-8106-01) is available now.
Contact our technical support team at techsupport@antibodiesinc.com or
(530) 758-4400 for expert guidance.