Products: Primary Antibodies

Anti-Tyrosine Hydroxylase (TYH) Antibody

Aves Labs Logo from Aves Labs

 Datasheet

Product Summary:

Two antipeptide antibodies were generated in chickens against sequences shared between the mouse (P24529) and human (P07101) gene products. Antibodies were affinity-purified and then mixed in equal concentrations. These antibodies have been validated with human, mouse and rat tissues.

PRODUCT OPTIONS

Brand Catalog # Option Volume Concentration Price
Brand
Aves Labs Logo
Catalog #
TYH
Volume
1000 µL
Concentration
200 µg/mL
Price
$399.00

Validation Images


Product Details

Antibody Type:

Polyclonal

Host Species:

Chicken

Species Reactivity:

Human Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

ICC IHC WB

Target Description:

Human TYH (EC 1.14.16.2) is a 58,523 dalton protein (528 amino acids) responsible for the enzymatic conversion of L-tyrosine to L-DOPA (dihydroxyphenylalanine). This enzyme is expressed in all catecholaminergic neurons of the CNS and PNS. In the CNS, TYH-positive neurons can be found within the substantia nigra, ventral tegmental area, locus ceruleus, and hypothalamus. In the PNS, TYH-positive neurons can be found within the sympathetic chain, pre-vertebral ganglia and the adrenal medulla.

Immunogen:

Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the Tyrosine Hydroxylase gene product, but were shared between the human (P07101, NCBI) and mouse (P24529, NCBI) sequences. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 200 ug/ml. Finally, equal volumes of both affinity-purified anti-peptide antibody preparations were mixed, and the preparation was filter-sterilized.

Protein Name(s):

Tyrosine 3-monooxygenase (EC 1.14.16.2) (Tyrosine 3-hydroxylase) (TH)

Gene Name(s):

TH TYH

Antibody Registry ID:

AB_10013440


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Molecular Weight:

60 kDa

Western Dilution Range:

1:1000-1:2000

IHC Dilution Range:

1:1000-1:2000


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Both of the antibodies were analyzed by immunohistochemistry (at a concentration of 3 ug/ml) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.


Citations and References