TrkB (pS478) Positive Control Cell Lysate (Mouse NSC-34)

Our Anti-Tyrosine Kinase Receptor B, phospho Ser478/479 (TrkB, pS478/479) cell lysate detects Tyrosine Kinase Receptor B, phospho Ser478/479 (TrkB, pS478/479), and is crude lysate. It is validated for use in WB.

Western blot analysis of TrkB pS478 expression in rodent brain homogenates (40 μg/lane) with R-1718-50, using CL-2107-50 as positive control lysate (10 μg/lane). SDS-PAGE: 4-12%, reducing conditions; Transfer: Towbin’s Tris-Glycine buffer, PVDF membrane (0.45 μm); Blocking buffer and antibody diluent: Mixture of 2.5% skim milk and 2.5% BSA in TBST, blocking 1 hour at RT; Primary antibody: 1 μg/mL, overnight at 2-8°C; Secondary antibody: anti-rabbit-HRP (1/10,000) 2.5 hours at RT; Detection: Chemiluminiscence. Tissue samples show expression of TrkB phosphorylated at serine residue 478 with a molecular weight of ~120 kDa. The retinoic acid (1 μM)- and BDNF-treated (50 ng/mL, 15 min) cell lysate (10 μg loading) serves as positive control supporting the assignment of the phosphorylated TrkB band. Additional bands are observed in tissue samples that have not yet been characterized.
Western blot analysis of TrkB pS478 expression in rodent brain homogenates (40 μg/lane) with R-1718-50, using CL-2107-50 as positive control lysate (10 μg/lane). SDS-PAGE: 4-12%, reducing conditions; Transfer: Towbin’s Tris-Glycine buffer, PVDF membrane (0.45 μm); Blocking buffer and antibody diluent: Mixture of 2.5% skim milk and 2.5% BSA in TBST, blocking 1 hour at RT; Primary antibody: 1 μg/mL, overnight at 2-8°C; Secondary antibody: anti-rabbit-HRP (1/10,000) 2.5 hours at RT; Detection: Chemiluminiscence. Tissue samples show expression of TrkB phosphorylated at serine residue 478 with a molecular weight of ~120 kDa. The retinoic acid (1 μM)- and BDNF-treated (50 ng/mL, 15 min) cell lysate (10 μg loading) serves as positive control supporting the assignment of the phosphorylated TrkB band. Additional bands are observed in tissue samples that have not yet been characterized.
Western blot analysis of TrkB pS478 expression in rodent brain homogenates (40 μg/lane) with R-1718-50, using CL-2107-50 as positive control lysate (10 μg/lane). SDS-PAGE: 4-12%, reducing conditions; Transfer: Towbin’s Tris-Glycine buffer, PVDF membrane (0.45 μm); Blocking buffer and antibody diluent: Mixture of 2.5% skim milk and 2.5% BSA in TBST, blocking 1 hour at RT; Primary antibody: 1 μg/mL, overnight at 2-8°C; Secondary antibody: anti-rabbit-HRP (1/10,000) 2.5 hours at RT; Detection: Chemiluminiscence.

Tissue samples show expression of TrkB phosphorylated at serine residue 478 with a molecular weight of ~120 kDa. The retinoic acid (1 μM)- and BDNF-treated (50 ng/mL, 15 min) cell lysate (10 μg loading) serves as positive control supporting the assignment of the phosphorylated TrkB band. Additional bands are observed in tissue samples that have not yet been characterized.
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Data Sheet

SKU: CL-2107-50

Volume/Size: 50 µg
Price:
$167.00

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