TrkB-Fc Chimera, Human, Purified Recombinant Protein

Name: TrkB-Fc Chimera, Human, Purified Recombinant Protein
Brand: Biosensis
SKU: PE-1235-20
Price: 347.0 USD
Availability: InStock

Tyrosine Kinase Receptor B (TrkB)-Fc Chimera, purified human recombinant protein, suitable for IP and to block.

Gel analysis of TrkB-Fc Chimera.

Lane 1: MW marker
Lane 2: TrkB-Fc Chimera (10 µg)
Lane 3: TrkB-Fc Chimera (10 µg) treated with PNGase F. Band at 35 kDa is PNGase F.

Click on image to zoom

Applications

Block, IP

Host Species

HEK293

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Data Sheet

SKU: PE-1235-20

Volume/Size: 20 µg

Variant

Price:

$347.00

Quantity:

Product Details

Target

Tyrosine Kinase Receptor B (TrkB)

Scientific Background

Tyrosine Kinase Receptor B (TrkB) is a high affinity receptor for brain-derived neurotrophic factor (BDNF) and neurotrophin-4/5. It also binds neurotrophin 3 (NT-3) with lower affinity. TrkB does not bind nerve growth factor (NGF). TrkB is involved in the development and/or maintenance of the nervous system and is widely expressed, predominantly in nervous tissue. TrkB is a heavily glycosylated type I transmembrane protein. The TrkB-Fc chimera contains 13 potential N-linked glycosylation sites.

Format

Purified. The chimera consists of 20-40% carbohydrate by weight.

Applications

Block, IP

Host Species

HEK293

Protein Sequence

A DNA sequence encoding the signal peptide and extracellular domain of human TrkB (amino acids 1- 428) was fused to the Fc region of human IgG1 (amino acids 93-330). The chimeric protein was expressed in modified human 293 cells.

Storage

Reconstitute with sterile-filtered phosphate-buffered saline to a concentration of 20 µg/mL. Lyophilized products should be stored at 2 to 8°C. Following reconstitution, short-term storage at 2-8°C is recommended and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.

Physical State

Lyophilized

Production Notes

>95% purity, as determined by SDS-PAGE and visualized by silver stain.

Application Details

The TrkB-Fc chimera bound to protein A sepharose beads is able to pull down its ligand BDNF. Optimal dilutions/concentrations should be determined by the end user.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

Tropomyosin-related kinase receptor; BDNF/NT-3 growth factors receptor; Neurotrophic tyrosine kinase receptor type 2; TrkB tyrosine kinase; GP145-TrkB/GP95-TrkB; Trk-B; Ntrk2; Trkb; TrkB-Fc Chimera

UniProt ID

Q16620

Shipping

25°C (ambient)

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TrkB-Fc Chimera, Human, Purified Recombinant Protein

SKU: PE-1235-20

Product Details

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