Products: Primary Antibodies

Anti-TRAK1 Antibody (N389/9)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-371	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-371	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2b

Species Reactivity:

Human Rat

Applications:

ICC IHC WB

Target Description:

Trafficking kinesin-binding protein 1 or O-Linked N-Acetylglucosamine Transferase Interacting Protein 106 is encoded by the gene TRAK1. TRAK1 is involved in mitochondrial motility and regulation of endosome-to-lysosome trafficking. TRAK1 can bind both kinesin and dynein. TRAK1 is ubiquitously expressed in tissues. Diseases associated with this gene include Early Infantile Epileptic Encephalopathy and Undetermined Early-Onset Epileptic Encephalopathy.

Immunogen:

Fusion protein amino acids 1-110 (N-terminus) of human TRAK1 (also known as Trafficking kinesinbinding protein 1, 106 kDa O-GlcNAc transferase-interacting protein, Ensembl ENSRNOP00000026106, KIAA1042, OIP106 and rCG_25699, accession number Q9UPV9)
Rat: 89% identity (98/110 amino acids identical)
Mouse: 88% identity (97/110 amino acids identical)
<50% identity with TRAK2

Cross Reactivity:

Does not cross-react with TRAK2

Protein Name(s):

Trafficking kinesin-binding protein 1 (106 kDa O-GlcNAc transferase-interacting protein)

Gene Name(s):

TRAK1 KIAA1042 OIP106

Antibody Registry ID:

Purified: AB_2315946
TC Supernatant: AB_2315945

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

100 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.