Products: Primary Antibodies

Anti-TARPGamma2/Stargazin (N245/1)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-242	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-242	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2a

Species Reactivity:

Mouse Rat

Applications:

ICC WB

Immunogen:

Fusion protein amino acids 203-323 (cytoplasmic C-terminus) of rat TARPGamma2 (also known as Voltage-dependent calcium channel gamma-2 subunit, Neuronal voltage-gated calcium channel gamma-2 subunit, Stargazin and Stg, accession number Q71RJ2)
Mouse: 100% identity (121/121 amino acids identical)
Human: 96% identity (117/121 amino acids identical) >50% identity with TARPGamma3, TARPGamma4 and TARPGamma8

Cross Reactivity:

Does not cross-react with TARPGamma3, TARPGamma4 and TARPGamma8

Protein Name(s):

Voltage-dependent calcium channel gamma-2 subunit (Neuronal voltage-gated calcium channel gamma-2 subunit) (Stargazin) (Transmembrane AMPAR regulatory protein gamma-2) (TARP gamma-2)

Gene Name(s):

Cacng2 Stg

Antibody Registry ID:

Purified: AB_11001587
TC Supernatant: AB_10675452

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Molecular Weight:

36 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Citations and References

Rademacher N1, Schmerl B1, Lardong JA2, Wahl MC2, Shoichet SA1.. (2016), 'MPP2 is a postsynaptic MAGUK scaffold protein that links SynCAM1 cell adhesion molecules to core components of the postsynaptic density..' Sci Rep.. 10.1038/srep35283.
Pandey SP1, Rai R, Gaur P, Prasad S.. (2015), 'Development- and age-related alterations in the expression of AMPA receptor subunit GluR2 and its trafficking proteins in the hippocampus of male mouse brain..' Biogerontology.. 10.1007/s10522-014-9548-6.
Maheshwari A1, Nahm WK, Noebels JL.. (2013), 'Paradoxical proepileptic response to NMDA receptor blockade linked to cortical interneuron defect in stargazer mice..' Front Cell Neurosci. . 10.3389/fncel.2013.00156.
Tao Y1, Chen YJ, Shen C, Luo Z, Bates CR, Lee D, Marchetto S, Gao TM, Borg JP, Xiong WC, Mei L.. (2013), 'Erbin interacts with TARP γ-2 for surface expression of AMPA receptors in cortical interneurons..' Nat Neurosci.. 10.1038/nn.3320.