Products: Primary Antibodies

Anti-SynDIG1/Tmem90b (L42/17)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-251	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-251	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2a

Species Reactivity:

Human Mouse Rat

Applications:

ICC WB

Immunogen:

Fusion protein amino acids 1-183 (cytoplasmic N-terminus) of mouse SynDIG1 (also known as Synapse differentiation-inducing gene protein 1, Dispanin subfamily C member 2, Transmembrane protein 90B, DSPC2, Tmem90b and C20orf39, accession number A2ANU3)
Rat: 97% identity (179/183 amino acids identical)
Human: 91% identity (167/183 amino acids identical)
50% identity with SynDIG1L/SynDIG2/Capucin/Tmem90a

Protein Name(s):

Synapse differentiation-inducing gene protein 1 (SynDIG1) (Dispanin subfamily C member 2) (DSPC2) (Transmembrane protein 90B)

Gene Name(s):

Syndig1 Tmem90b

Antibody Registry ID:

Purified: AB_10999753
TC Supernatant: AB_11000166

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

36 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Citations and References

Kirk LM1, Ti SW2, Bishop HI2, Orozco-Llamas M1, Pham M1, Trimmer JS2,3, Díaz E1.. (2016), 'Distribution of the SynDIG4/proline-rich transmembrane protein 1 in rat brain..' J Comp Neurol.. 10.1002/cne.23945.
Sumioka A1, Brown TE, Kato AS, Bredt DS, Kauer JA, Tomita S.. (2011), 'PDZ binding of TARPγ-8 controls synaptic transmission but not synaptic plasticity..' Nat Neurosci.. 10.1038/nn.2952.
Kalashnikova E1, Lorca RA, Kaur I, Barisone GA, Li B, Ishimaru T, Trimmer JS, Mohapatra DP, Díaz E.. (2010), 'SynDIG1: an activity-regulated, AMPA- receptor-interacting transmembrane protein that regulates excitatory synapse development..' Neuron.. 10.1016/j.neuron.2009.12.021.