Products: Primary Antibodies

Anti-SynCAM1 (L45/30)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-120	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-120	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:

ICC WB

Immunogen:

Fusion protein amino acids 257-388 of mouse SynCAM1 (also known as synaptic cell adhesion molecule 1, immunoglobulin superfamily member 4, nectin-like protein 2 or NECL-2, tumor suppressor in lung cancer 1 or TSLC-1, spermatogenic immunoglobulin superfamily or SgIGSF, Igsf4 and Ra175, accession number Q8R5M8), recognizes 1.4b isoform only (epitope narrowed to GLTQLPNSAEELDSEDLS)
Rat: 96% identity (129/134 amino acids identical)
Human: 89% identity (118/132 amino acids identical)<50% identity with other SynCAM’s

Cross Reactivity:

No cross-reactivity with SynCAM2 or SynCAM3

Protein Name(s):

Cell adhesion molecule 1 (Immunoglobulin superfamily member 4) (IgSF4) (Nectin-like protein 2) (NECL-2) (Spermatogenic immunoglobulin superfamily) (SgIgSF) (Synaptic cell adhesion molecule) (SynCAM) (Tumor suppressor in lung cancer 1) (TSLC-1)

Gene Name(s):

Cadm1 Igsf4 Necl2 Ra175 Syncam SynCam1 Tslc1

Antibody Registry ID:

Purified: AB_2068036
TC Supernatant: AB_10671665

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

50 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.

Citations and References

Rollenhagen M, Kuckuck S, Ulm C, Hartmann M, Galuska SP, Geyer R, Geyer H, Mühlenhoff M.. (2012), 'Polysialylation of the synaptic cell adhesion molecule 1 (SynCAM 1) depends exclusively on the polysialyltransferase ST8SiaII in vivo. .' The Journal of Biological Chemistry. 10.1074/jbc.M112.375642.