Products: Primary Antibodies

Anti-Shank2 (N23B/6)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-088	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-088	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:

IHC IP WB

Immunogen:

Fusion protein amino acids 84-309 (SH3/PDZ domains) of rat Shank2 (SH3 and multiple ankyrin repeat domains protein 2; accession number Q9QX74)

Human: 94% identity (213/226 amino acids identical)
Mouse: 97% identity (220/226 amino acids identical)

Cross Reactivity:

No cross-reactivity against Shank1 or Shank3

Protein Name(s):

SH3 and multiple ankyrin repeat domains protein 2 (Shank2) (Cortactin-binding protein 1) (CortBP1) (GKAP/SAPAP-interacting protein) (Proline-rich synapse-associated protein 1) (ProSAP1) (SPANK-3)

Gene Name(s):

Shank2 Cortbp1

Antibody Registry ID:

Purified: AB_2254586
TC Supernatant: AB_10674119

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

160 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Citations and References

MacGillavry HD,,, Kerr JM,, Kassner J, Frost NA,,, Blanpied TA.. (2016), 'Shank-cortactin interactions control actin dynamics to maintain flexibility of neuronal spines and synapses. .' European Journal of Neuroscience. 10.1111/ejn.13129.
Tao-Cheng JH, Azzam R, Crocker V, Winters CA, Reese T.. (2015), 'Depolarization of Hippocampal Neurons Induces Formation of Nonsynaptic NMDA Receptor Islands Resembling Nascent Postsynaptic Densities. .' eNeuro. 10.1523/ENEURO.0066-15.2015.
Farley MM, Swulius MT, Waxham MN.. (2015), 'Electron tomographic structure and protein composition of isolated rat cerebellar, hippocampal and cortical postsynaptic densities. .' Neuroscience. 10.1016/j.neuroscience.2015.07.062.
Braude JP, Vijayakumar S, Baumgarner K, Laurine R, Jones TA, Jones SM, Pyott SJ.. (), 'Deletion of Shank1 has minimal effects on the molecular composition and function of glutamatergic afferent postsynapses in the mouse inner ear. .' Hearing Research. 10.1016/j.heares.2015.01.008.
Peykov S, Berkel S, Schoen M, Weiss K, Degenhardt F, Strohmaier J, Weiss B, Proepper C, Schratt G, Nöthen MM,, Boeckers TM, Rietschel M, Rappold GA.. (), 'Identification and functional characterization of rare SHANK2 variants in schizophrenia. .' Molecular Psychiatry. 10.1038/mp.2014.172.
Schneider K, Seemann E, Liebmann L, Ahuja R, Koch D, Westermann M, Hübner CA, Kessels MM, Qualmann B.. (2014), 'ProSAP1 and membrane nanodomain-associated syndapin I promote postsynapse formation and function. .' The Journal of Biology. 10.1083/jcb.201307088.
Baple EL, Maroofian R, Chioza BA, Izadi M, Cross HE, Al-Turki S, Barwick K, Skrzypiec A, Pawlak R, Wagner K, Coblentz R, Zainy T, Patton MA, Mansour S, Rich P, Qualmann B, Hurles ME, Kessels MM, Crosby AH.. (), 'Mutations in KPTN cause macrocephaly, neurodevelopmental delay, and seizures. .' Am J Human Genetics. 10.1016/j.ajhg.2013.10.001.
Han K, Holder JL Jr, Schaaf CP, Lu H, Chen H, Kang H, Tang J, Wu Z, Hao S, Cheung SW, Yu P, Sun H, Breman AM, Patel A, Lu HC, Zoghbi HY.. (2013), 'SHANK3 overexpression causes manic-like behaviour with unique pharmacogenetic properties. .' Nature. 10.1038/nature12630.
Qian H, Matt L, Zhang M, Nguyen M, Patriarchi T, Koval OM, Anderson ME, He K, Lee HK, Hell JW.. (2012), 'β2-Adrenergic receptor supports prolonged theta tetanus-induced LTP. .' Journal of Neurophysiology. 10.1152/jn.00374.2011.
Berkel S, Tang W, Treviño M, Vogt M, Obenhaus HA, Gass P, Scherer SW, Sprengel R, Schratt G, Rappold GA.. (), 'Inherited and de novo SHANK2 variants associated with autism spectrum disorder impair neuronal morphogenesis and physiology. .' Human Molecular Genetics. 10.1093/hmg/ddr470.
Verpelli C, Dvoretskova E, Vicidomini C, Rossi F, Chiappalone M, Schoen M, Di Stefano B, Mantegazza R, Broccoli V, Böckers TM, Dityatev A, Sala C.. (2011), 'Importance of Shank3 protein in regulating metabotropic glutamate receptor 5 (mGluR5) expression and signaling at synapses. .' The Journal of Biological Chemistry. 10.1074/jbc.M111.258384.
Xie M, Rich TC, Scheitrum C, Conti M, Richter W.. (), 'Inactivation of multidrug resistance proteins disrupts both cellular extrusion and intracellular degradation of cAMP. .' Molecular Pharmacology. 10.1124/mol.111.071134.
Tao-Cheng JH, Dosemeci A, Gallant PE, Smith C, Reese T.. (), 'Activity induced changes in the distribution of Shanks at hippocampal synapses. .' Neuroscience. 10.1016/j.neuroscience.2010.03.041.