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Products: Primary Antibodies

Anti-Shank2 Antibody (N23B/6)

NeuroMab Logo from NeuroMab

 Datasheet

PRODUCT OPTIONS

Brand Catalog # Option Volume Concentration Price
Brand
NeuroMab Logo
Catalog #
75-088
Option
Purified
Volume
100 µL
Concentration
1 mg/mL
Price
$275.00
Brand
NeuroMab Logo
Catalog #
73-088
Option
TC Supernatant
Volume
5 mL
Concentration
Lot dependent
Price
$275.00

Validation Images


Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

IHC IP WB

Target Description:

SH3 and multiple ankyrin repeat domains protein 2, or shank 2 is a member of the shank family. Shank 2 binding domains include a SH3 domain, a proline rich domain, and a PDZ domain. Shank 2 is found in kidney, liver tissue, and epithelial cells. In brain, Shank 2 is expressed at the apical cell membrane, cytoplasm, synapse, postsynaptic density, growth cone, and dendritic spine. Some isoforms of shank 2 are expressed during development, while others are expressed in mature tissue. Shank 2 acts as a scaffold protein anchoring and connecting membrane bound receptors, including NMDA and glutamate receptors and other proteins to the cytoskeleton in neurons to enable cell signaling. In this manner, Shank 2 may play a role in synapse formation and dendritic spine maturation. Mutations in the Shank 2 gene have been associated with autism spectrm disorder and blount disease.

Immunogen:

Fusion protein amino acids 84-309 (SH3/PDZ domains) of rat Shank2 (SH3 and multiple ankyrin repeat domains protein 2; accession number Q9QX74); Human: 94% identity (213/226 amino acids identical); Mouse: 97% identity (220/226 amino acids identical)

Cross Reactivity:

No cross-reactivity against Shank1 or Shank3

Protein Name(s):

Shank2

Gene Name(s):

Shank2 Cortbp1

Antibody Registry ID:

Purified: AB_2254586
TC Supernatant: AB_10674119


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:
This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

160 kDa


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.