Polyhistidine Anti-6XHIS (for Westerns) Antibody

Name: Polyhistidine Anti-6XHIS (for Westerns) Antibody
Brand: Aves Labs
SKU: HIS-W-1010
Price: 402.0 USD
Availability: InStock

Our anti-6XHIS antibody recognizes the 6X-HIS tag with high titre and high specificity in western blot applications.

Anti-Polyhistidine Anti-6XHIS (for Western) Antibody

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Host Species

Chicken

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Data Sheet

SKU: HIS-W-1010

Volume: 100 µL

Variant

Price:

$180.00

Quantity:

Ships: 1-2 business days

Product Details

Target

6XHIS

Scientific Background

The synthetic peptide 6X-Histidine (HHHHHH).

Format

Mixture of IgY fraction and affinity-purified antibodies

Concentration

10 mg/mL

Clonality

Polyclonal

Isotype

IgY

Host Species

Chicken

Immunogen

Synthetic peptide

Antibody Registry ID

AB_2313518

Storage

Store at -20°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile.

Physical State

Liquid

Production Notes

Chickens were immunized with the 6X-Histidine peptide conjugated to keyhole limpet hemocyanin protein and emulsified in Freund’s adjuvants. After multiple injections, eggs were collected from the hens, IgY fractions were prepared from the yolks, and then affinity-purified antibodies were prepared using 6X-Histidine peptide conjugated to an agarose matrix. To make up the final mixture, the IgY fraction was spiked with affinity purified antibody, mixed with glycerol and sodium azide, and filter-sterilized.

Buffer

Sodium phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline ("PBS," pH 7.2) (50% by volume) with glycerol (50% by volume) as an anti-freezing agent and sodium azide (0.02%, w/v) as a preservative.

Dilution Ranges

IHC: 1:5000-1:10000

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from receipt of product

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Polyhistidine Anti-6XHIS (for Westerns) Antibody

SKU: HIS-W-1010

Product Details

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