ICC IHC WB
Sodium/potassium/calcium exchanger 4, Na(+)/K(+)/Ca(2+)-Exchange Protein 4 or NCKX4 is encoded by the gene SLC24A4. NCKX4 is a member of the potassium-dependent sodium/calcium exchanger protein family. NCKX4 is thought to play roles in olfactory function and in tooth enamel formation. It is expressed in the aorta, lung, thymus and brain, particularly enriched in neurons. Diseases associated with SLC24A4 include cause amelogenesis imperfecta.
Fusion protein amino acids 246-424 (third intracellular loop) of human NCKX4 isoform 3 (also known as Sodium/potassium/calcium exchanger 4, Na(+)/K(+)/Ca(2+)- exchange protein 4, Solute carrier family 24 member 4 and SLC24A4, accession number Q8NFF2-3)
Human: 90% identity with isoform 1 (179/198 amino acids identical)
Mouse: 89% identity with isoform 2 and 81% identity with isoform 1 (161/179 and 161/198 amino acids identical)
Rat: 81% identity (161/198 amino acids identical)
40% identity with NCKX3 and other NCKX proteins
Does not cross-react with NCKX2 or NCKX3
Sodium/potassium/calcium exchanger 4 (Na(+)/K(+)/Ca(2+)-exchange protein 4) (Solute carrier family 24 member 4)
TC Supernatant: AB_2491078
Antibody Validation and Application Notes
This antibody has been validated using the following assays:
This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).
This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.
This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.
This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.
Citations and References
- Vinberg F1, Wang T2,3,4, De Maria A1, Zhao H5, Bassnett S1, Chen J2,3,4, Kefalov VJ1.. (2017), 'The Na+/Ca2+, K+ exchanger NCKX4 is required for efficient cone-mediated vision..' Elife.. 10.7554/eLife.24550.
- Bronckers AL1, Jalali R2, Lytton J3.. (2017), 'Reduced Protein Expression of the Na+/Ca2++K+-Exchanger (SLC24A4) in Apical Plasma Membranes of Maturation Ameloblasts of Fluorotic Mice..' Calcif Tissue Int.. 10.1007/s00223-016-0197-4.