Anti-NCKX4 Antibody (N414/25)

Name: Anti-NCKX4 Antibody
Brand: NeuroMab
SKU: 75-404
Price: 319.0 USD
Availability: InStock

Our Anti-NCKX4 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N414/25. It is KO validated, detects human and rat NCKX4, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

Immunoblot against membranes from wild- type (N4+/+) or NKCX4 knockout (N4-/-) mouse brains probed with N414/25 TC supe. Data courtesy of Sunita Sharma and Jonathan Lytton (University of Calgary).

Adult rat hippocampus immunohistochemistry.

Immunoblot against extracts of COS cells transiently transfected with Flag- tagged NCKX2, NCKX3 or NCKX4 plasmid and probed with N414/25 (left) or N52A/42 (right) TC supe.

Immunostaining of NCKX4 (cat. 75-404; red) along the trajectory of ameloblast differentiation, from dental epithelial stem cells residing in the cervical loop, secretory, transition, to ruffle-ended and smooth-ended maturation ameloblasts, fully coexisting in the continuously growing mouse incisors. Nuclei are labeled with DAPI (blue). Image from publication CC-BY-4.0. PMID:36814474

Immunoblot against membranes from wild- type (N4+/+) or NKCX4 knockout (N4-/-) mouse brains probed with N414/25 TC supe. Data courtesy of Sunita Sharma and Jonathan Lytton (University of Calgary).

Species Reactivity

Human, Mouse, Rat, Zebrafish

Applications

ICC, IHC, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

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Data Sheet

KO Validated

SKU: 75-404

Volume: 100 µL

Variant

Price:

$319

Quantity:

Product Details

Target

NCKX4

Target Description

Sodium/potassium/calcium exchanger 4, Na(+)/K(+)/Ca(2+)-Exchange Protein 4 or NCKX4 is encoded by the gene SLC24A4. NCKX4 is a member of the potassium-dependent sodium/calcium exchanger protein family. NCKX4 is thought to play roles in olfactory function and in tooth enamel formation. It is expressed in the aorta, lung, thymus and brain, particularly enriched in neurons. Diseases associated with SLC24A4 include cause amelogenesis imperfecta.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N414/25

Isotype

IgG2b

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

SLC24A4 NCKX4

Molecular Weight

60 kDa

Antigen

Fusion protein amino acids 246-424 (third intracellular loop) of human NCKX4 isoform 3 (accession number Q8NFF2-3) produced recombinantly in E. Coli

Species Reactivity

Human, Mouse, Rat, Zebrafish

Antibody Registry ID

AB_2491110

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Dilution Ranges

WB: 1:500
IHC: 1:500

Conjugate

Unconjugated

Specificity

Does not cross-react with NCKX2 or NCKX3

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Sodium/potassium/calcium exchanger 4 (Na(+)/K(+)/Ca(2+)-exchange protein 4) (Solute carrier family 24 member 4)

UniProt Details

UniProt (Human): Q8NFF2
UniProt (Immunogen Species): Q8NFF2-3

Product Specific References for Applications and Species

Immunohistochemistry: Mouse | Zebrafish
Western Blot: Mouse | Zebrafish

Immunohistochemistry: Mouse
PMID	Dilution	Publication
36814474	not listed	Chan, B., et al. 2023. Sodium/(calcium + potassium) Exchanger NCKX4 Optimizes KLK4 Activity in the Enamel Matrix Microenvironment to Regulate ECM Modeling. Frontiers in Physiology, 1116091.
33199372	1 : 500	Thibodeau, S., et al. 2020. Calmodulin binds and modulates K +-dependent Na +/Ca 2+-exchanger isoform 4, NCKX4. Journal of Biological Chemistry, 100092.
29209227	not listed	Jalali, R., et al. 2017. The Role of Na:K:2Cl Cotransporter 1 (NKCC1/SLC12A2) in Dental Epithelium during Enamel Formation in Mice. Frontiers in Physiology, 924.
27752731	not listed	Bronckers, A.L., et al. 2017. Reduced Protein Expression of the Na+/Ca2++K+-Exchanger (SLC24A4) in Apical Plasma Membranes of Maturation Ameloblasts of Fluorotic Mice.. Calcified Tissue International, 80-86.

Immunohistochemistry: Zebrafish
PMID	Dilution	Publication
28650316	not listed	Vinberg, F., et al. 2017. The Na+/Ca2+, K+ exchanger NCKX4 is required for efficient cone-mediated vision.. Elife, e24550.

Western Blot: Mouse
PMID	Dilution	Publication
33199372	1 : 500	Thibodeau, S., et al. 2020. Calmodulin binds and modulates K +-dependent Na +/Ca 2+-exchanger isoform 4, NCKX4. Journal of Biological Chemistry, 100092.
29209227	not listed	Jalali, R., et al. 2017. The Role of Na:K:2Cl Cotransporter 1 (NKCC1/SLC12A2) in Dental Epithelium during Enamel Formation in Mice. Frontiers in Physiology, 924.
27752731	not listed	Bronckers, A.L., et al. 2017. Reduced Protein Expression of the Na+/Ca2++K+-Exchanger (SLC24A4) in Apical Plasma Membranes of Maturation Ameloblasts of Fluorotic Mice.. Calcified Tissue International, 80-86.

Western Blot: Zebrafish
PMID	Dilution	Publication
28650316	not listed	Vinberg, F., et al. 2017. The Na+/Ca2+, K+ exchanger NCKX4 is required for efficient cone-mediated vision.. Elife, e24550.

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Anti-NCKX4 Antibody (N414/25)

SKU: 75-404

Product Details

Product Specific References for Applications and Species

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