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Products: Primary Antibodies

Anti-NCKX4 (N414/25)


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PRODUCT OPTIONS

Catalog # Option Volume Concentration Price*
Catalog #
75-404
Option
Purified
Volume
100 µL
Concentration
1 mg/mL
Price
$269.00
Catalog #
73-404
Option
TC Supernatant
Volume
5 mL
Concentration
Lot dependent
Price
$269.00

Validation Images


Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2b

Species Reactivity:

Human Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

ICC IHC WB

Immunogen:

Fusion protein amino acids 246-424 (third intracellular loop) of human NCKX4 isoform 3 (also known as Sodium/potassium/calcium exchanger 4, Na(+)/K(+)/Ca(2+)- exchange protein 4, Solute carrier family 24 member 4 and SLC24A4, accession number Q8NFF2-3)
Human: 90% identity with isoform 1 (179/198 amino acids identical)
Mouse: 89% identity with isoform 2 and 81% identity with isoform 1 (161/179 and 161/198 amino acids identical)
Rat: 81% identity (161/198 amino acids identical)
40% identity with NCKX3 and other NCKX proteins

Cross Reactivity:

Does not cross-react with NCKX2 or NCKX3

Protein Name(s):

Sodium/potassium/calcium exchanger 4 (Na(+)/K(+)/Ca(2+)-exchange protein 4) (Solute carrier family 24 member 4)

Gene Name(s):

SLC24A4 NCKX4

Antibody Registry ID:

Purified: AB_2491110
TC Supernatant: AB_2491078


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:
This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Expected Banding Pattern:

60 kDa


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.


Citations and References