Products: Primary Antibodies

Anti-MECP2 Antibody (N227/21)

NeuroMab Logo from NeuroMab

 Datasheet

PRODUCT OPTIONS

Brand Catalog # Option Volume Concentration Price
Brand
NeuroMab Logo
Catalog #
75-273
Option
Purified
Volume
100 µL
Concentration
1 mg/mL
Price
$275.00
Brand
NeuroMab Logo
Catalog #
73-273
Option
TC Supernatant
Volume
5 mL
Concentration
Lot dependent
Price
$275.00

Validation Images


Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

DB IHC

Target Description:

MeCP2 (Methyl-CpG Binding Protein 2) is a chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair and is not influenced by sequences flanking the methyl-CpGs. MeCP2 has been shown to mediate transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A (Nan et al., 1998). Defects in MeCP2 are the cause of Rett syndrome (RTT) (Amir et al., 1999). RTT is an X-linked dominant disease; it is a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.

Immunogen:

Synthetic peptide amino acids 157-169 (FTVTG[dimethyl-R]GSPSRRE) of human MECP2 (also known as Methyl-CpG-binding protein 2, accession number P51608)
Mouse: 100% identity (13/13 amino acids identical)
Rat: 100% identity (13/13 amino acids identical)

Cross Reactivity:

Reacts preferentially with dimethylated peptide than with unmethylated peptide

Protein Name(s):

Methyl-CpG-binding protein 2 (MeCp-2 protein) (MeCp2)

Gene Name(s):

MECP2

Antibody Registry ID:

Purified: AB_11001583
TC Supernatant: AB_11001717


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Immunohistochemistry:
This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.