Products: Primary Antibodies

Anti-LRRTM4 (N205B/22)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-261	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-261	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:

ICC WB

Immunogen:

Synthetic peptide amino acids 470-484 (ARESERQMNSPLQEY, cytoplasmic C-terminus) of human LRRTM4 (also known as Leucine-rich repeat transmembrane neuronal protein 4, accession number Q86VH4)
Mouse: 100% identity (15/15 amino acids identical)
Rat: 100% identity (15/15 amino acids identical) 100% identity between Long and Short isoforms <50% identity with LRRTM1, LRRTM2 and LRRTM3

Cross Reactivity:

Reacts with both Long and Short isoforms

Protein Name(s):

Leucine-rich repeat transmembrane neuronal protein 4

Gene Name(s):

LRRTM4 UNQ3075/PRO9907

Antibody Registry ID:

Purified: AB_10674580
TC Supernatant: AB_10674105

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Molecular Weight:

70 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Citations and References

de Wit J1, O'Sullivan ML, Savas JN, Condomitti G, Caccese MC, Vennekens KM, Yates JR 3rd, Ghosh A.. (2013), 'Unbiased discovery of glypican as a receptor for LRRTM4 in regulating excitatory synapse development..' Neuron.. 10.1016/j.neuron.2013.06.049.