Products: Primary Antibodies

Anti-Kv4.3 K+ channel (K75/41)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-017	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $269.00
Catalog # 73-017	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $269.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat Xenopus

Applications:

IHC IP WB

Immunogen:

Fusion protein amino acids 415-636 (cytoplasmic C- terminus) of rat Kv4.3 (accession number Q62897)
Human: 99% identity (203/205 amino acids)
Mouse: 98% identity (201/205 amino acids)

Cross Reactivity:

No cross-reactivity against rat Kv4.2 expressed in transfected cells.

Protein Name(s):

Potassium voltage-gated channel subfamily D member 3 (Voltage-gated potassium channel subunit Kv4.3)

Gene Name(s):

Kcnd3

Antibody Registry ID:

Purified: AB_2131966
TC Supernatant: AB_10672856

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Expected Banding Pattern:

75 kDa in rat brain membrane preparations

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Citations and References

Huang CY, Chu D, Hwang WC, Tsaur ML.. (2012), 'Coexpression of high-voltage-activated ion channels Kv3.4 and Cav1.2 in pioneer axons during pathfinding in the developing rat forebrain..' Journal of Comparative Neurology. 10.1002/cne.23119.
Zhang X,, Ai X,, Nakayama H, Chen B, Harris DM, Tang M, Xie Y, Szeto C, Li Y, Li Y,, Zhang H, Eckhart AD, Koch WJ, Molkentin JD, Chen X.. (2016), 'Persistent increases in Ca(2+) influx through Cav1.2 shortens action potential and causes Ca(2+) overload-induced afterdepolarizations and arrhythmias. .' Basic Research in Cardiology. 10.1007/s00395-015-0523-4.
Cheng CF, Wang WC, Huang CY, Du PH, Yang JH, Tsaur ML.. (2016), 'Coexpression of auxiliary subunits KChIP and DPPL in potassium channel Kv4-positive nociceptors and pain-modulating spinal interneurons. .' Journal of Comparative Neurology. 10.1002/cne.23876.
Simkin D, Hattori S, Ybarra N, Musial TF, Buss EW, Richter H, Oh MM, Nicholson DA, Disterhoft JF.. (2015), 'Aging-Related Hyperexcitability in CA3 Pyramidal Neurons Is Mediated by Enhanced A-Type K+ Channel Function and Expression. .' Journal of Neuroscience. 10.1523/JNEUROSCI.0193-15.2015.
Liu J, Kim KH, Morales MJ, Heximer SP, Hui CC, Backx PH.. (2015), 'Kv4.3-Encoded Fast Transient Outward Current Is Presented in Kv4.2 Knockout Mouse Cardiomyocytes. .' PLoS One. 10.1371/journal.pone.0133274.
Cheng CF, Cheng JK, Chen CY, Rau RH, Chang YC, Tsaur ML.. (2015), 'Nerve growth factor-induced synapse-like structures in contralateral sensory ganglia contribute to chronic mirror-image pain..' Pain. 10.1097/j.pain.0000000000000280.
You T, Mao W, Cai B, Li F, Xu H.. (2015), 'Two novel Brugada syndrome-associated mutations increase KV4.3 membrane expression and function..' International Journal of Molecular Medicine. 10.3892/ijmm.2015.2223.
Duarri A, Lin MC, Fokkens MR, Meijer M, Smeets CJ, Nibbeling EA, Boddeke E, Sinke RJ, Kampinga HH, Papazian DM, Verbeek DS.. (2015), 'Spinocerebellar ataxia type 19/22 mutations alter heterocomplex Kv4.3 channel function and gating in a dominant manner. .' Cell Mol Life Sciences. 10.1007/s00018-015-1894-2.
Lei Z, Zhang H, Liang Y, Cui Q, Xu Z, Xu ZC.. (2014), 'Reduced expression of IA channels is associated with postischemic seizures in hyperglycemic rats. .' Journal of Neuroscience Research. 10.1002/jnr.23445.
Dufour MA, Woodhouse A, Goaillard JM.. (2014), 'Somatodendritic ion channel expression in substantia nigra pars compacta dopaminergic neurons across postnatal development. .' Journal of Neuroscience Research. 10.1002/jnr.23382.
Rainnie DG, Hazra R, Dabrowska J, Guo JD, Li CC, Dewitt S, Muly EC.. (2014), 'Distribution and functional expression of Kv4 family α subunits and associated KChIP β subunits in the bed nucleus of the stria terminalis. .' Journal of Comparative Neurology. 10.1002/cne.23435.
Duarri A, Nibbeling E, Fokkens MR, Meijer M, Boddeke E, Lagrange E, Stevanin G, Brice A, Durr A, Verbeek DS.. (2013), 'The L450F [Corrected] mutation in KCND3 brings spinocerebellar ataxia and Brugada syndrome closer together. .' NeuroGenetics. 10.1007/s10048-013-0370-0.
David JP, Andersen MN, Olesen SP, Rasmussen HB, Schmitt N.. (2013), 'Trafficking of the IKs -complex in MDCK cells: site of subunit assembly and determinants of polarized localization. .' Traffic. 10.1111/tra.12042.
Duarri A, Jezierska J, Fokkens M, Meijer M, Schelhaas HJ, den Dunnen WF, van Dijk F, Verschuuren-Bemelmans C, Hageman G, van de Vlies P, Küsters B, van de Warrenburg BP, Kremer B, Wijmenga C, Sinke RJ, Swertz MA, Kampinga HH, Boddeke E, Verbeek DS.. (2012), 'Mutations in potassium channel kcnd3 cause spinocerebellar ataxia type 19. .' Annals of Neurology. 10.1002/ana.23700.
Yao JJ, Gao XF, Chow CW, Zhan XQ, Hu CL, Mei YA.. (2012), 'Neuritin activates insulin receptor pathway to up-regulate Kv4.2-mediated transient outward K+ current in rat cerebellar granule neurons. .' The Journal of Biological Chemistry. 10.1074/jbc.M112.390260.
Matsuyoshi H, Takimoto K, Yunoki T, Erickson VL, Tyagi P, Hirao Y, Wanaka A, Yoshimura N.. (2012), 'Distinct cellular distributions of Kv4 pore-forming and auxiliary subunits in rat dorsal root ganglion neurons..' Life Sciences. 10.1016/j.lfs.2012.07.007.
Menegola M, Clark E, Trimmer JS.. (2012), 'The importance of immunohistochemical analyses in evaluating the phenotype of Kv channel knockout mice. .' Epilepsia. 10.1111/j.1528-1167.2012.03485.x.
Sethi J, Sanchez-Alavez M, Tabarean IV.. (2011), 'Kv4.2 mediates histamine modulation of preoptic neuron activity and body temperature..' PLoS One. 10.1371/journal.pone.0029134.
Zhang YH, Wu W, Sun HY, Deng XL, Cheng LC, Li X, Tse HF, Lau CP, Li GR.. (2012), 'Modulation of human cardiac transient outward potassium current by EGFR tyrosine kinase and Src-family kinases. .' Cardiovascular Research. 10.1093/cvr/cvr347.
Zhang M, Wang Y, Jiang M, Zankov DP, Chowdhury S, Kasirajan V, Tseng GN.. (2012), 'KCNE2 protein is more abundant in ventricles than in atria and can accelerate hERG protein degradation in a phosphorylation-dependent manner. .' American Journal of Physiology-Heart and Circulatory Physiology. 10.1152/ajpheart.00691.2011.
Marshall GE, Russell JA, Tellez JO, Jhund PS, Currie S, Dempster J, Boyett MR, Kane KA, Rankin AC, Workman AJ.. (2012), 'Remodelling of human atrial K+ currents but not ion channel expression by chronic β-blockade..' Pflügers Archiv: European Journal of Physiology. 10.1007/s00424-011-1061-z.
Sun W, Maffie JK, Lin L, Petralia RS, Rudy B, Hoffman DA.. (2011), 'DPP6 establishes the A-type K(+) current gradient critical for the regulation of dendritic excitability in CA1 hippocampal neurons. .' Neuron. 10.1016/j.neuron.2011.08.008.
Lei Z, Deng P, Li J, Xu ZC.. (2012), 'Alterations of A-type potassium channels in hippocampal neurons after traumatic brain injury. .' Journal of Neurotrauma. 10.1089/neu.2010.1537.
Hourez R, Servais L, Orduz D, Gall D, Millard I, de Kerchove d'Exaerde A, Cheron G, Orr HT, Pandolfo M, Schiffmann SN.. (2011), 'Aminopyridines correct early dysfunction and delay neurodegeneration in a mouse model of spinocerebellar ataxia type 1. .' Journal of Neuroscience. 10.1523/JNEUROSCI.0905-11.2011.
Wykes R, Kalmbach A, Eliava M, Waters J.. (2012), 'Changes in the physiology of CA1 hippocampal pyramidal neurons in preplaque CRND8 mice. .' Neurobiology of Aging. 10.1016/j.neurobiolaging.2011.05.001.
Stein AB, Jones TA, Herron TJ, Patel SR, Day SM, Noujaim SF, Milstein ML, Klos M, Furspan PB, Jalife J, Dressler GR.. (2011), 'Loss of H3K4 methylation destabilizes gene expression patterns and physiological functions in adult murine cardiomyocytes. .' Journal of Clinical Investigation. 10.1172/JCI44641.
Cheng L, Yung A, Covarrubias M, Radice GL.. (2011), 'Cortactin is required for N-cadherin regulation of Kv1.5 channel function. .' The Journal of Biological Chemistry. 10.1074/jbc.M111.218560.
Guan D, Horton LR, Armstrong WE, Foehring RC.. (2011), 'Postnatal development of A-type and Kv1- and Kv2-mediated potassium channel currents in neocortical pyramidal neurons. .' Journal of Neurophysiology. 10.1152/jn.00758.2010.
Nowak A, Mathieson HR, Chapman RJ, Janzsó G, Yanagawa Y, Obata K, Szabo G, King AE.. (2011), 'Kv3.1b and Kv3.3 channel subunit expression in murine spinal dorsal horn GABAergic interneurones. .' Journal of Chemical Neuroanatomy. 10.1016/j.jchemneu.2011.02.003.
Prüss H, Dewes M, Derst C, Fernández-Klett F, Veh RW, Priller J.. (2011), 'Potassium channel expression in adult murine neural progenitor cells. .' Neuroscience. 10.1016/j.neuroscience.2011.02.021.
Schwetz TA, Norring SA, Ednie AR, Bennett ES.. (2011), 'Sialic acids attached to O-glycans modulate voltage-gated potassium channel gating. .' The Journal of Biological Chemistry. 10.1074/jbc.M110.171322.
Norris AJ, Foeger NC, Nerbonne JM.. (2010), 'Interdependent roles for accessory KChIP2, KChIP3, and KChIP4 subunits in the generation of Kv4-encoded IA channels in cortical pyramidal neurons..' Journal of Neuroscience. 10.1523/JNEUROSCI.2487-10.2010.
Dabrowska J, Rainnie DG.. (2010), 'Expression and distribution of Kv4 potassium channel subunits and potassium channel interacting proteins in subpopulations of interneurons in the basolateral amygdala..' Neuroscience. 10.1016/j.neuroscience.2010.09.011.
Bealer SL, Little JG, Metcalf CS, Brewster AL, Anderson AE.. (2010), 'Autonomic and cellular mechanisms mediating detrimental cardiac effects of status epilepticus. .' Epilepsy Research. 10.1016/j.eplepsyres.2010.06.013.
Norris AJ, Nerbonne JM.. (2010), 'Molecular dissection of I(A) in cortical pyramidal neurons reveals three distinct components encoded by Kv4.2, Kv4.3, and Kv1.4 alpha-subunits. .' Journal of Neuroscience. 10.1523/JNEUROSCI.5890-09.2010.
Cotella D, Radicke S, Bortoluzzi A, Ravens U, Wettwer E, Santoro C, Sblattero D.. (2010), 'Impaired glycosylation blocks DPP10 cell surface expression and alters the electrophysiology of Ito channel complex. .' Pflügers Archiv: European Journal of Physiology. 10.1007/s00424-010-0824-2.
Phuket TR, Covarrubias M.. (2009), 'Kv4 Channels Underlie the Subthreshold-Operating A-type K-current in Nociceptive Dorsal Root Ganglion Neurons. .' Frontiers in Molecular Neuroscience. 10.3389/neuro.02.003.2009.
Jiang M, Xu X, Wang Y, Toyoda F, Liu XS, Zhang M, Robinson RB, Tseng GN.. (2009), 'Dynamic partnership between KCNQ1 and KCNE1 and influence on cardiac IKs current amplitude by KCNE2. .' The Journal of Biological Chemistry. 10.1074/jbc.M808262200.
Eyre MD, Kerti K, Nusser Z.. (2009), 'Molecular diversity of deep short-axon cells of the rat main olfactory bulb. .' European Journal of Neuroscience. 10.1111/j.1460-9568.2009.06703.x.
Clark BD, Kwon E, Maffie J, Jeong HY, Nadal M, Strop P, Rudy B.. (2008), 'DPP6 Localization in Brain Supports Function as a Kv4 Channel Associated Protein..' Frontiers in Molecular Neuroscience. 10.3389/neuro.02.008.2008.
Monaghan MM, Menegola M, Vacher H, Rhodes KJ, Trimmer JS.. (2008), 'Altered expression and localization of hippocampal A-type potassium channel subunits in the pilocarpine-induced model of temporal lobe epilepsy. .' Neuroscience. 10.1016/j.neuroscience.2008.07.057.
Schwenk J, Zolles G, Kandias NG, Neubauer I, Kalbacher H, Covarrubias M, Fakler B, Bentrop D.. (2008), 'NMR analysis of KChIP4a reveals structural basis for control of surface expression of Kv4 channel complexes. .' The Journal of Biological Chemistry. 10.1074/jbc.M800976200.
Amarillo Y, De Santiago-Castillo JA, Dougherty K, Maffie J, Kwon E, Covarrubias M, Rudy B.. (2008), 'Ternary Kv4.2 channels recapitulate voltage-dependent inactivation kinetics of A-type K+ channels in cerebellar granule neurons. .' The Journal of Physiology. 10.1113/jphysiol.2007.150540.
Chien LY, Cheng JK, Chu D, Cheng CF, Tsaur ML.. (2007), 'Reduced expression of A-type potassium channels in primary sensory neurons induces mechanical hypersensitivity. .' Journal of Neuroscience. 10.1523/JNEUROSCI.0604-07.2007.
Burkhalter A, Gonchar Y, Mellor RL, Nerbonne JM.. (2006), 'Differential expression of I(A) channel subunits Kv4.2 and Kv4.3 in mouse visual cortical neurons and synapses. .' Journal of Neuroscience. 10.1523/JNEUROSCI.2599-06.2006.
Bourdeau ML, Morin F, Laurent CE, Azzi M, Lacaille JC.. (2007), 'Kv4.3-mediated A-type K+ currents underlie rhythmic activity in hippocampal interneurons..' Journal of Neuroscience. 10.1523/JNEUROSCI.3208-06.2007.
Menegola M, Trimmer JS.. (2006), 'Unanticipated region- and cell-specific downregulation of individual KChIP auxiliary subunit isotypes in Kv4.2 knock-out mouse brain..' Journal of Neuroscience. 10.1523/JNEUROSCI.2783-06.2006.
Kollo M, Holderith NB, Nusser Z.. (2006), 'Novel subcellular distribution pattern of A-type K+ channels on neuronal surface. .' Journal of Neuroscience. 10.1523/JNEUROSCI.5257-05.2006.