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Products: Primary Antibodies

Anti-Kv4.3 K+ channel Antibody (K75/41)

NeuroMab Logo from NeuroMab

 Datasheet

PRODUCT OPTIONS

Brand Catalog # Option Volume Concentration Price
Brand
NeuroMab Logo
Catalog #
75-017
Option
Purified
Volume
100 µL
Concentration
1 mg/mL
Price
$325.00
Brand
NeuroMab Logo
Catalog #
73-017
Option
TC Supernatant
Volume
5 mL
Concentration
Lot dependent
Price
$325.00

Validation Images


Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat Xenopus

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

IHC IP WB

Target Description:

Kv4.3 K+ channel (Potassium voltage-gated channel subfamily D member 3 ) is a member of the mammalian Shal-related family and the potassium voltage gated channel subunit family. Kv4 subunit family includes four Kv4 a subunits (Kv4.1, Kv4.2 and Kv4.3). However only Kv4.2 and Kv4.3 are predominantely epressed in the brain (Alfaro-Ruiz et al., 2005). KV4.3 subunit is encoded by gene KCND3 in human. It has been demonstrated that Kv 4.2 and Kv4.3 are often colocalized with pain-modulating molecules in rat spinal lamina II excitatory interneurons (Huang et al., 2005).

Immunogen:

Fusion protein amino acids 415-636 (cytoplasmic C- terminus) of rat Kv4.3 (accession number Q62897); Human: 99% identity (203/205 amino acids); Mouse: 98% identity (201/205 amino acids)

Cross Reactivity:

No cross-reactivity against rat Kv4.2 expressed in transfected cells.

Protein Name(s):

Kv4.3 K+ channel

Gene Name(s):

Kcnd3

Antibody Registry ID:

Purified: AB_2131966
TC Supernatant: AB_10672856


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:
This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

75 kDa in rat brain membrane preparations


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.