Products: Primary Antibodies

Anti-Kv3.3 potassium channel (N375/67)


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PRODUCT OPTIONS

Catalog # Option Volume Concentration Price*
Catalog #
75-354
Option
Purified
Volume
100 µL
Concentration
1 mg/mL
Price
$205.00
Catalog #
73-354
Option
TC Supernatant
Volume
5 mL
Concentration
Lot dependent
Price
$180.00

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

ICC IHC WB

Immunogen:

Fusion protein amino acids 628-728 (cytoplasmic C-terminus) of mouse Kv3.3b (also known as Potassium voltage-gated channel subfamily C member 3, KSHIIID and Kcnc3, accession number Q63959) Human: 96% identity (94/97 amino acids identical) Rat: 90% identity (96/106 amino acids identical) 100% identity with Kv3.3a for first 30 amino acids (AGGLGIMGLPPLPAPGEPCPLEEVIETNRA) <40% identity with Kv3.4 or Kv3.2

Cross Reactivity:

Does not cross-react with Kv3.4 or Kv3.2

Protein Name(s):

Potassium voltage-gated channel subfamily C member 3 (KSHIIID) (Voltage-gated potassium channel subunit Kv3.3)

Gene Name(s):

Kcnc3

Antibody Registry ID:

Purified: AB_2315872
TC Supernatant: AB_2315871


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:
This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Expected Banding Pattern:

80-100 kDa (depending on isoform)


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.