Products: Primary Antibodies

Anti-Kirrel3 short and long (N321C/49)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-333	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Catalog # 73-333	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2b

Species Reactivity:

Mouse Rat

Applications:

ICC WB

Immunogen:

Fusion protein amino acids 46-524 (extracellular N-terminus) of rat Kirrel3 (also known as Kin of irregular chiasm-like protein 3, Kin of IRRE-like protein 3, Nephrin-like protein 2, NEPH2 and Kiaa1867, accession number Q09GS6) Mouse: 97% identity (479/491 amino acids identical) Human: 95% identity (470/491 amino acids identical) 100% identity between short and long isoforms ~50% identity with Kirrel1 and Kirrel2

Cross Reactivity:

Reacts with Kirrel3 short and long isoforms Does not cross-react with Kirrel1 and Kirrel2

Protein Name(s):

Membrane protein KIRRE

Gene Name(s):

Kirrel3 Kirrel3_predicted

Antibody Registry ID:

Purified: AB_2315857
TC Supernatant: AB_2315856

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Molecular Weight:

85-105 kDa (multiple isoforms)

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.