Products: Primary Antibodies

Anti-JMJD2A (N154/32)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-189	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $269.00
Catalog # 73-189	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $269.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human

Applications:

ICC WB

Immunogen:

Fusion protein amino acids 568-674 of human JMJD2A (also known as JmjC domain-containing histone demethylation protein 3A, Jumonji domain-containing protein 2A, JHDM3A and KIAA0677, accession number O75164)
Mouse: 94% identity (100/106 amino acids identical)
>50% identity with JMJD2C

Cross Reactivity:

Does not cross-react with JMJD2C

Protein Name(s):

Lysine-specific demethylase 4A (EC 1.14.11.-) (JmjC domain-containing histone demethylation protein 3A) (Jumonji domain-containing protein 2A)

Gene Name(s):

KDM4A JHDM3A JMJD2 JMJD2A KIAA0677

Antibody Registry ID:

Purified: AB_10671303
TC Supernatant: AB_10671822

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Molecular Weight:

120 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.

Citations and References

Carbonneau M,, M Gagné L, Lalonde ME, Germain MA, Motorina A,, Guiot MC,, Secco B,, Vincent EE,, Tumber A,1, Hulea L1,1, Bergeman J, Oppermann U,1, Jones RG,, Laplante M,, Topisirovic I1,1, Petrecca K, Huot MÉ,1, Mallette FA,,1.. (2016), 'The oncometabolite 2-hydroxyglutarate activates the mTOR signalling pathway. .' Nature Communications. 10.1038/ncomms12700.
Neault M, Mallette FA, Richard S.. (), 'miR-137 Modulates a Tumor Suppressor Network-Inducing Senescence in Pancreatic Cancer Cells. .' Cell Report. 10.1016/j.celrep.2016.01.068.
Satterlee JS, Beckel-Mitchener A, McAllister K, Procaccini DC, Rutter JL, Tyson FL, Chadwick LH.. (2015), 'Community resources and technologies developed through the NIH Roadmap Epigenomics Program. .' Methods Mol Biol.. 10.1007/978-1-4939-1804-1_2.
Van Rechem C, Black JC, Abbas T, Allen A, Rinehart CA, Yuan GC, Dutta A, Whetstine JR. (2011), 'The SKP1-Cul1-F-box and leucine-rich repeat protein 4 (SCF-FbxL4) ubiquitin ligase regulates lysine demethylase 4A (KDM4A)/Jumonji domain-containing 2A (JMJD2A) protein. .' The Journal of Biological Chemistry. 10.1074/jbc.M111.273508.
Black JC, Allen A, Van Rechem C, Forbes E, Longworth M, Tschöp K, Rinehart C, Quiton J, Walsh R, Smallwood A, Dyson NJ, Whetstine JR.. (2010), 'Conserved antagonism between JMJD2A/KDM4A and HP1γ during cell cycle progression. .' Molecular Cell. 10.1016/j.molcel.2010.11.008.