Products: Primary Antibodies

Anti-JMJD2A Antibody (N154/32)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-189	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-189	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human

Applications:

ICC WB

Target Description:

Lysine Demethylase 4A is encoded by the gene KDM4A. KDM4A is a member of the JHDM3 histone demethylase family. KDM4A is a nuclear protein that contains a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. KDM4A functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor. KDM4A is expressed is several organs with the highest expression in the lungs. Diseases associated with KDM4A include Attention Deficit-Hyperactivity Disorder.

Immunogen:

Fusion protein amino acids 568-674 of human JMJD2A (also known as JmjC domain-containing histone demethylation protein 3A, Jumonji domain-containing protein 2A, JHDM3A and KIAA0677, accession number O75164); Mouse: 94% identity (100/106 amino acids identical); Greater than 50% identity with JMJD2C

Cross Reactivity:

Does not cross-react with JMJD2C

Protein Name(s):

JMJD2A

Gene Name(s):

KDM4A JHDM3A JMJD2 JMJD2A KIAA0677

Antibody Registry ID:

Purified: AB_10671303
TC Supernatant: AB_10671822

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Molecular Weight:

120 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.