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Products: Primary Antibodies

Anti-HCN4 Antibody (N114/10)

NeuroMab Logo from NeuroMab

 Datasheet

PRODUCT OPTIONS

Brand Catalog # Option Volume Concentration Price
Brand
NeuroMab Logo
Catalog #
75-150
Option
Purified
Volume
100 µL
Concentration
1 mg/mL
Price
$275.00
Brand
NeuroMab Logo
Catalog #
73-150
Option
TC Supernatant
Volume
5 mL
Concentration
Lot dependent
Price
$275.00

Validation Images


Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

ICC IHC WB

Target Description:

HCN4 is a member of the hyperpolarization-activated cyclic nucleotide gated channel family, also known as pacemaker channels. This family has a role in rhythmic activity of the heart and firing neurons. HCN channels are made up of four subunits, HCN1-4 and can be formed in multiple combinations. HCN4 is found in the cell membrane as a multi pass membrane protein in the sino-atrial node of the heart. This protein is highly expressed in neurons in the thalamus and other brain regions and testis. Mutations in HCN4 have been found in several types of sinus bradycardia.

Immunogen:

Fusion protein amino acids 1019-1108 (cytoplasmic Cterminus) of rat HCN4(also known as potassium/sodium hyperpolarization-activated cyclic nucleotidegated channel 4, brain cyclic nucleotide-gated channel 3 and BCNG-3, accession number Q9JKA7); Mouse: 100% identity (90/90 amino acids identical); Human: 100% identity (90/90 amino acids identical); Less than 50% identity with HCN1, HCN2 and HCN3

Cross Reactivity:

No cross-reactivity against other HCN’s

Protein Name(s):

HCN4

Gene Name(s):

HCN4

Antibody Registry ID:

Purified: AB_2248534
TC Supernatant: AB_10673158


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:
This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

130 kDa


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.