Products: Primary Antibodies

Anti-HCN4 Antibody (N114/10)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-150	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-150	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:

ICC IHC WB

Target Description:

HCN4 is a member of the hyperpolarization-activated cyclic nucleotide gated channel family, also known as pacemaker channels. This family has a role in rhythmic activity of the heart and firing neurons. HCN channels are made up of four subunits, HCN1-4 and can be formed in multiple combinations. HCN4 is found in the cell membrane as a multi pass membrane protein in the sino-atrial node of the heart. This protein is highly expressed in neurons in the thalamus and other brain regions and testis. Mutations in HCN4 have been found in several types of sinus bradycardia.

Immunogen:

Fusion protein amino acids 1019-1108 (cytoplasmic Cterminus) of rat HCN4(also known as potassium/sodium hyperpolarization-activated cyclic nucleotidegated channel 4, brain cyclic nucleotide-gated channel 3 and BCNG-3, accession number Q9JKA7); Mouse: 100% identity (90/90 amino acids identical); Human: 100% identity (90/90 amino acids identical); Less than 50% identity with HCN1, HCN2 and HCN3

Cross Reactivity:

No cross-reactivity against other HCN’s

Protein Name(s):

HCN4

Gene Name(s):

HCN4

Antibody Registry ID:

Purified: AB_2248534
TC Supernatant: AB_10673158

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

130 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.