Products: Primary Antibodies

Anti-HCN4 (N114/10)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-150	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Catalog # 73-150	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:

ICC IHC WB

Immunogen:

Fusion protein amino acids 1019-1108 (cytoplasmic Cterminus) of rat HCN4(also known as potassium/sodium hyperpolarization-activated cyclic nucleotidegated channel 4, brain cyclic nucleotide-gated channel 3 and BCNG-3, accession number Q9JKA7)

Mouse: 100% identity (90/90 amino acids identical)
Human: 100% identity (90/90 amino acids identical)
<50% identity with HCN1, HCN2 and HCN3

Cross Reactivity:

No cross-reactivity against other HCN’s

Protein Name(s):

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4

Gene Name(s):

HCN4

Antibody Registry ID:

Purified: AB_2248534
TC Supernatant: AB_10673158

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

130 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Citations and References

Krishnaswamy A#, Yamagata M#, Duan X, Hong YK, Sanes JR.. (2015), 'Sidekick 2 directs formation of a retinal circuit that detects differential motion. .' Nature. 10.1038/nature14682.
Stradleigh TW, Ogata G, Partida GJ, Oi H, Greenberg KP, Krempely KS, Ishida AT.. (2011), 'Colocalization of hyperpolarization-activated, cyclic nucleotide-gated channel subunits in rat retinal ganglion cells. .' Journal of Comparative Neurology. 10.1002/cne.22638.
Hellmer CB, Zhou Y, Fyk-Kolodziej B, Hu Z, Ichinose T.. (2016), 'Morphological and physiological analysis of type-5 and other bipolar cells in the Mouse Retina. .' Neuroscience. 10.1016/j.neuroscience.2015.12.016.
Farshi P, Fyk-Kolodziej B, Krolewski DM, Walker PD, Ichinose T.. (2016), 'Dopamine D1 receptor expression is bipolar cell type-specific in the mouse retina. .' Journal of Comparative Neurology. 10.1002/cne.23932.
Ichinose T,, Hellmer CB.. (2016), 'Differential signalling and glutamate receptor compositions in the OFF bipolar cell types in the mouse retina. .' The Journal of Physiology. 10.1113/JP271458.
Stradleigh TW, Ishida AT.. (2016), 'Fixation strategies for retinal immunohistochemistry. .' Progress in Retinal and Eye Research. 10.1016/j.preteyeres.2015.04.001.
Nam YJ, Lubczyk C, Bhakta M, Zang T, Fernandez-Perez A, McAnally J, Bassel-Duby R, Olson EN, Munshi NV.. (2016), 'Induction of diverse cardiac cell types by reprogramming fibroblasts with cardiac transcription factors. .' Development. 10.1242/dev.114025.
Mesirca P, Alig J, Torrente AG, Müller JC, Marger L, Rollin A, Marquilly C, Vincent A, Dubel S, Bidaud I, Fernandez A, Seniuk A, Engeland B, Singh J, Miquerol L, Ehmke H, Eschenhagen T, Nargeot J, Wickman K, Isbrandt D1, Mangoni ME1.. (2014), 'Cardiac arrhythmia induced by genetic silencing of 'funny' (f) channels is rescued by GIRK4 inactivation. .' Nature Communications. 10.1038/ncomms5664.
Viney TJ#, Lasztoczi B#,, Katona L#, Crump MG#, Tukker JJ, Klausberger T,, Somogyi P.. (2013), 'Network state-dependent inhibition of identified hippocampal CA3 axo-axonic cells in vivo. .' Nature Neuroscience. 10.1038/nn.3550.
Stillitano F, Lonardo G, Giunti G, Del Lungo M, Coppini R, Spinelli V, Sartiani L, Poggesi C, Mugelli A, Cerbai E.. (2013), 'Chronic atrial fibrillation alters the functional properties of If in the human atrium. .' J Cardiovasc Electrophysiol.. 10.1111/jce.12212.
Stoenica L, Wilkars W, Battefeld A, Stadler K, Bender R, Strauss U.. (2013), 'HCN1 subunits contribute to the kinetics of I(h) in neonatal cortical plate neurons. .' Developmental Neurobiology. 10.1002/dneu.22104.
Al Madhoun AS, Voronova A, Ryan T, Zakariyah A, McIntire C, Gibson L, Shelton M, Ruel M, Skerjanc IS.. (2013), 'Testosterone enhances cardiomyogenesis in stem cells and recruits the androgen receptor to the MEF2C and HCN4 genes. .' Journal of Molecular and Cellular Cardiology. 10.1016/j.yjmcc.2013.04.003.
Abbas SY, Hamade KC, Yang EJ, Nawy S, Smith RG, Pettit DL.. (2013), 'Directional summation in non-direction selective retinal ganglion cells. .' PLOS Computational Biology. 10.1371/journal.pcbi.1002969.
Hughes DI, Boyle KA, Kinnon CM, Bilsland C, Quayle JA, Callister RJ, Graham BA.. (2013), 'HCN4 subunit expression in fast-spiking interneurons of the rat spinal cord and hippocampus. .' Neuroscience. 10.1016/j.neuroscience.2013.01.028.
Ying SW, Kanda VA, Hu Z, Purtell K, King EC, Abbott GW, Goldstein PA.. (2012), 'Targeted deletion of Kcne2 impairs HCN channel function in mouse thalamocortical circuits. .' PLoS One. 10.1371/journal.pone.0042756.
Almanza A, Luis E, Mercado F, Vega R, Soto E.. (2012), 'Molecular identity, ontogeny, and cAMP modulation of the hyperpolarization-activated current in vestibular ganglion neurons. .' Journal of Neurophysiology. 10.1152/jn.00337.2012.
Hughes DI, Sikander S, Kinnon CM, Boyle KA, Watanabe M, Callister RJ, Graham BA.. (2012), 'Morphological, neurochemical and electrophysiological features of parvalbumin-expressing cells: a likely source of axo-axonic inputs in the mouse spinal dorsal horn. .' The Journal of Physiology. 10.1113/jphysiol.2012.235655.
Khurana S, Liu Z, Lewis AS, Rosa K, Chetkovich D, Golding NL.. (2012), 'An essential role for modulation of hyperpolarization-activated current in the development of binaural temporal precision. .' Journal of Neuroscience. 10.1523/JNEUROSCI.3882-11.2012.
Partida GJ, Stradleigh TW, Ogata G, Godzdanker I, Ishida AT.. (2012), 'Thy1 associates with the cation channel subunit HCN4 in adult rat retina. .' Investigative Ophthalmology & Visual Science. 10.1167/iovs.11-9307.
Kanyshkova T, Meuth P, Bista P, Liu Z, Ehling P, Caputi L, Doengi M, Chetkovich DM, Pape HC, Budde T.. (2011), 'Differential regulation of HCN channel isoform expression in thalamic neurons of epileptic and non-epileptic rat strains. .' Neurobiology of Disease. 10.1016/j.nbd.2011.08.032.
Anderson WD, Galván EJ, Mauna JC, Thiels E, Barrionuevo G.. (2011), 'Properties and functional implications of I (h) in hippocampal area CA3 interneurons. .' Pflügers Archiv: European Journal of Physiology. 10.1007/s00424-011-1025-3.
Giesbrecht CJ, Mackay JP, Silveira HB, Urban JH, Colmers WF.. (2010), 'Countervailing modulation of Ih by neuropeptide Y and corticotrophin-releasing factor in basolateral amygdala as a possible mechanism for their effects on stress-related behaviors. .' Journal of Neuroscience. 10.1523/JNEUROSCI.2306-10.2010.
Li Y, Serwanski DR, Miralles CP, Fiondella CG, Loturco JJ, Rubio ME, De Blas AL.. (2010), 'Synaptic and nonsynaptic localization of protocadherin-gammaC5 in the rat brain. .' Journal of Comparative Neurology. 10.1002/cne.22390.