Products: Primary Antibodies

Anti-Gs protein, alpha subunit Antibody (N192/12)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-211	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-211	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Mouse Rat

Applications:

Target Description:

Guanine nucleotide-binding protein G(s) subunit alpha isoforms short or Gs protein a subunit is encoded by the gene GNAS. Gsa is one of three subunits that make up a guanine nucleotide binding protein (Gprotein). The other proteins are a beta and a gamma subunit. G proteins are further divided into families depending on the type of a subunit included, G(S)alpha,G(Q)alpha,G(I)alpha,and G(12)alpha. Gsa functions as a transducer of numerous signaling pathways that are controlled by g protein coupled receptors (GPCRs) by coupling receptors to adenylyl cyclase and results in increased levels of the signaling molecule cAMP. Diseases associated with GNAS include Mccune-Albright Syndrome and Progressive Osseous Heteroplasia.

Immunogen:

Fusion protein amino acids 1-380 (full-length, short form) of bovine Gs protein alpha subunit(also known as Guanine nucleotide-binding protein Gs subunit alpha isoforms short, GNAS, GNAS1, GSP, G-alpha-8 and Adenylate cyclase-stimulating G alpha protein, accession number P04896-2); Human: 99% identity (379/380 amino acids identical); Mouse: 99% identity (378/380 amino acids identical); Rat: 95% identity (377/394 amino acids identical); Significant identity with multiple Guanine nucleotide-binding proteins

Cross Reactivity:

Reacts with both Active and Inactive/QL forms

Protein Name(s):

Gs protein, alpha subunit

Gene Name(s):

GNAS GNAS1

Antibody Registry ID:

Purified: AB_2315846
TC Supernatant: AB_2315845

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Molecular Weight:

45 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.