Products: Primary Antibodies

Anti-Gs protein, alpha subunit (N192/12)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-211	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $269.00
Catalog # 73-211	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $269.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Mouse Rat

Applications:

Immunogen:

Fusion protein amino acids 1-380 (full-length, short form) of bovine Gs protein alpha subunit(also known as Guanine nucleotide-binding protein Gs subunit alpha isoforms short, GNAS, GNAS1, GSP, G-alpha-8 and Adenylate cyclase-stimulating G alpha protein, accession number P04896-2)
Human: 99% identity (379/380 amino acids identical)
Mouse: 99% identity (378/380 amino acids identical)
Rat: 95% identity (377/394 amino acids identical)
Significant identity with multiple Guanine nucleotide-binding proteins

Cross Reactivity:

Reacts with both Active and Inactive/QL forms

Protein Name(s):

Guanine nucleotide-binding protein G(s) subunit alpha isoforms short (Adenylate cyclase-stimulating G alpha protein)

Gene Name(s):

GNAS GNAS1

Antibody Registry ID:

Purified: AB_2315846
TC Supernatant: AB_2315845

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Expected Banding Pattern:

45 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Citations and References

Donati RJ,, Schappi J, Czysz AH, Jackson A, Rasenick MM.. (2015), 'Differential effects of antidepressants escitalopram versus lithium on Gs alpha membrane relocalization. .' BMC Neuroscience. 10.1186/s12868-015-0178-y.
Sun Z, Runne C, Tang X, Lin F, Chen S.. (2012), 'The Gβ3 splice variant associated with the C825T gene polymorphism is an unstable and functionally inactive protein. .' Cellular Signalling. 10.1016/j.cellsig.2012.08.011.
Loo L, Shepherd AJ, Mickle AD, Lorca RA, Shutov LP, Usachev YM, Mohapatra DP.. (2012), 'The C-type natriuretic peptide induces thermal hyperalgesia through a noncanonical Gβγ-dependent modulation of TRPV1 channel. .' Journal of Neuroscience. 10.1523/JNEUROSCI.1330-12.2012.
Soltysinska E, Thiele S, Olesen SP, Osadchii OE.. (2011), 'Chronic sympathetic activation promotes downregulation of β-adrenoceptor-mediated effects in the guinea pig heart independently of structural remodeling and systolic dysfunction. .' Pflügers Archiv: European Journal of Physiology. 10.1007/s00424-011-1005-7.