Products: Primary Antibodies

Anti-Gamma-protocadherin-A3 (N144/17)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-177	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $269.00
Catalog # 73-177	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $269.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Mouse

Applications:

ICC WB

Immunogen:

Fusion protein amino acids 720-804 (variable cytoplasmic domain) of mouse Gamma-protocadherin-A3 (also known as PCDH-gamma-A3, accession number Q91XY5)
Human: 80% identity (68/84 amino acids identical)
>75% identity with other Gamma-protocadherin-A proteins

Cross Reactivity:

No cross-reactivity against other Gamma-protocadherin-A, -B or -C proteins

Protein Name(s):

Protocadherin gamma A3 (Protocadherin gamma subfamily A, 3)

Gene Name(s):

Pcdhga3

Antibody Registry ID:

Purified: AB_10671815
TC Supernatant: AB_10697874

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Expected Banding Pattern:

100 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.

Citations and References

Chen WV, Alvarez FJ, Lefebvre JL, Friedman B, Nwakeze C, Geiman E, Smith C, Thu CA, Tapia JC, Tasic B, Sanes JR, Maniatis T.. (2012), 'Functional significance of isoform diversification in the protocadherin gamma gene cluster. .' Neuron. 10.1016/j.neuron.2012.06.039.
Lobas MA, Helsper L, Vernon CG, Schreiner D, Zhang Y, Holtzman MJ, Thedens DR, Weiner JA.. (), 'Molecular heterogeneity in the choroid plexus epithelium: the 22-member γ-protocadherin family is differentially expressed, apically localized, and implicated in CSF regulation. .' Journal of Neurochemistry. 10.1111/j.1471-4159.2011.07587.x.