Products: Primary Antibodies

Anti-Cav3.1 Ca2+ channel (N178A/9)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-206	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-206	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:

ICC IHC WB

Immunogen:

Fusion protein amino acids 2052-2172 (cytoplasmic C-terminus) of mouse Cav3.1 (also known as Voltage-gated/dependent T-type calcium channel alpha-1-G subunit, Cacna1g and NBR13, accession number Q9WUT2)

Rat: 96% identity (117/121 amino acids identical)
Human: 80% identity (98/122 amino acids identical)
30% identity with Cav3.2

Protein Name(s):

Voltage-dependent T-type calcium channel subunit alpha

Gene Name(s):

Cacna1g

Antibody Registry ID:

Purified: AB_2069421
TC Supernatant: AB_10673097

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

>200 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Citations and References

Devergnas A, Chen E, Ma Y, Hamada I, Pittard D, Kammermeier S, Mullin AP, Faundez V, Lindsley CW, Jones C, Smith Y, Wichmann T.. (2016), 'Anatomical localization of Cav3.1 calcium channels and electrophysiological effects of T-type calcium channel blockade in the motor thalamus of MPTP-treated monkeys. .' Journal of Neurophysiology. 10.1152/jn.00858.2015.
Curran J, Musa H, Kline CF, Makara MA, Little SC, Higgins JD, Hund TJ, Band H, Mohler PJ.. (2015), 'Eps15 Homology Domain-containing Protein 3 Regulates Cardiac T-type Ca2+ Channel Targeting and Function in the Atria. .' The Journal of Biological Chemistry. 10.1074/jbc.M115.646893.
Puthussery T, Venkataramani S, Gayet-Primo J, Smith RG, Taylor WR.. (2013), 'NaV1.1 channels in axon initial segments of bipolar cells augment input to magnocellular visual pathways in the primate retina. .' Journal of Neuroscience. 10.1523/JNEUROSCI.1249-13.2013.
Valerie NC, Dziegielewska B, Hosing AS, Augustin E, Gray LS, Brautigan DL, Larner JM, Dziegielewski J.. (2013), 'Inhibition of T-type calcium channels disrupts Akt signaling and promotes apoptosis in glioblastoma cells. .' Biochem Pharmacol.. 10.1016/j.bcp.2012.12.017.
Goonasekera SA, Hammer K, Auger-Messier M, Bodi I, Chen X, Zhang H, Reiken S, Elrod JW, Correll RN, York AJ, Sargent MA, Hofmann F, Moosmang S, Marks AR, Houser SR, Bers DM, Molkentin JD.. (2012), 'Decreased cardiac L-type Ca²⁺ channel activity induces hypertrophy and heart failure in mice. .' Journal of Clinical Investigation. 10.1172/JCI58227.