Products: Primary Antibodies

Anti-Cav3.1 Ca2+ channel Antibody (N178A/9)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-206	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-206	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:

ICC IHC WB

Target Description:

Voltage-dependent T-type calcium channel subunit alpha or Cav3.1 Ca2+ channel is encoded by the gene CACNA1G. Cav3.1 is a pore forming alpha subunit of T voltage-sensitive calcium channels and gives rise to T-type calcium currents. (transient opening calcium channels). These channels facilitate entry of calcium ions into excitable cells are involved in a variety of calcium dependent processes, including neurotransmitter release, muscle contraction, and gene expression. Cav3.1 is expressed in various tissues including heart and smooth muscle, kidney, bone, and brain as a membrane bound protein. Diseases associated with this gene include forms of Spinocerebellar Ataxia

Immunogen:

Fusion protein amino acids 2052-2172 (cytoplasmic C-terminus) of mouse Cav3.1 (also known as Voltage-gated/dependent T-type calcium channel alpha-1-G subunit, Cacna1g and NBR13, accession number Q9WUT2); Rat: 96% identity (117/121 amino acids identical); Human: 80% identity (98/122 amino acids identical); 30% identity with Cav3.2

Protein Name(s):

Cav3.1 Ca2+ channel

Gene Name(s):

Cacna1g

Antibody Registry ID:

Purified: AB_2069421
TC Supernatant: AB_10673097

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

>200 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.