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Products: Primary Antibodies

Anti-Beta1-spectrin Antibody (N385/21)

NeuroMab Logo from NeuroMab

 Datasheet

PRODUCT OPTIONS

Brand Catalog # Option Volume Concentration Price
Brand
NeuroMab Logo
Catalog #
75-374
Option
Purified
Volume
100 µL
Concentration
1 mg/mL
Price
$275.00
Brand
NeuroMab Logo
Catalog #
73-374
Option
TC Supernatant
Volume
5 mL
Concentration
Lot dependent
Price
$275.00

Validation Images


Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2b

Species Reactivity:

Human Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

ICC IHC WB

Target Description:

Spectrin beta chain, erythrocytic, Beta1 spectrin, or Membrane Cytoskeletal Protein is encoded by the gene SPTB and is a member of the member of the spectrin gene family. Beta1 spectrin is primary member of the cytoskeletal network in the erythrocyte plasma membrane. Beta1 spectrin is involved in cell membrane organization and stability and associates with the proteins band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane. Diseases associated with this gene include Spherocytosis and Elliptocytosis.

Immunogen:

Fusion protein amino acids 1555-2137 (C-terminal repeats 13 to 17) of human Beta1-spectrin (also known as Erythrocytic spectrin beta chain, SPTB, SPTB1, Spnb-1, Spnb1 and LOC314251, accession number P11277)
Mouse: 90% identity (530/538 amino acids identical)
Rat: 90% identity (530/538 amino acids identical)
50% identity with Beta2-spectrin

Protein Name(s):

Spectrin beta chain, erythrocytic (Beta-I spectrin)

Gene Name(s):

SPTB SPTB1

Antibody Registry ID:

Purified: AB_2315815
TC Supernatant: AB_2315814


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:
This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

250 kDa


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.