Products: Primary Antibodies

Anti-Beta1-spectrin Antibody (N385/21)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-374	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-374	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2b

Species Reactivity:

Human Mouse Rat

Applications:

ICC IHC WB

Target Description:

Spectrin beta chain, erythrocytic, Beta1 spectrin, or Membrane Cytoskeletal Protein is encoded by the gene SPTB and is a member of the member of the spectrin gene family. Beta1 spectrin is primary member of the cytoskeletal network in the erythrocyte plasma membrane. Beta1 spectrin is involved in cell membrane organization and stability and associates with the proteins band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane. Diseases associated with this gene include Spherocytosis and Elliptocytosis.

Immunogen:

Fusion protein amino acids 1555-2137 (C-terminal repeats 13 to 17) of human Beta1-spectrin (also known as Erythrocytic spectrin beta chain, SPTB, SPTB1, Spnb-1, Spnb1 and LOC314251, accession number P11277)
Mouse: 90% identity (530/538 amino acids identical)
Rat: 90% identity (530/538 amino acids identical)
50% identity with Beta2-spectrin

Protein Name(s):

Spectrin beta chain, erythrocytic (Beta-I spectrin)

Gene Name(s):

SPTB SPTB1

Antibody Registry ID:

Purified: AB_2315815
TC Supernatant: AB_2315814

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

250 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.