Human Mouse Rat
ICC IHC WB
Actin Like 6B, of BAFcomplex 53 KDa Subunit (BAF53b) is encoded by the gene ACTL6B and is a member of actin-related proteins family. It is a subunit of the neuron specific chromatin remodeling complex (nBAF complex). ACTL6B plays a role in remodeling mononucleosomes in an ATP-dependent fashion, and is required for postmitotic neural development and dendritic outgrowth. Diseases associated with ACTL6B include Early Infantile Epileptic Encephalopathy 76 and Intellectual Developmental Disorder with Severe Speech and Ambulation Defects.
Fusion protein amino acids 39-114 (TTVGLLAAEEGGGLELEGDKEKKGKIFHIDTNALHVPRDGAEV
MSPLKNGMIEDWECFRAILDHTYSKHVKSEPNL, actin subdomain 2) of human BAF53b (also known as 53 kDa BRG1-associated factor B, BRG1-associated factor 53B, Actin-like protein 6B, ArpNalpha, ACTL6B and ACTL6, accession number O94805)
Mouse: 98% identity (75/76 amino acids identical)
Rat: 98% identity (75/76 amino acids identical)
>50% identity with BAF53a
Does not cross-react with BAF53a
Actin-like protein 6B (53 kDa BRG1-associated factor B) (Actin-related protein Baf53b) (ArpNalpha) (BRG1-associated factor 53B) (BAF53B)
ACTL6B ACTL6 BAF53B
TC Supernatant: AB_2315810
Antibody Validation and Application Notes
This antibody has been validated using the following assays:
This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).
This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.
This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.
This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
Each new lot of this antibody is tested to confirm that it shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.
Citations and References
- Vogel Ciernia A1, Kramár EA2,3, Matheos DP2,3, Havekes R4, Hemstedt TJ2,3, Magnan CN5,6, Sakata K2,3, Tran A2,3, Azzawi S2,3, Lopez A2,3, Dang R2,3, Wang W5,6, Trieu B7,8, Tong J2,3, Barrett RM9, Post RJ2,3, Baldi P5,6, Abel T10, Lynch G8, Wood MA2,3,6.. (2017), 'Mutation of neuron-specific chromatin remodeling subunit BAF53b: rescue of plasticity and memory by manipulating actin remodeling..' Learn Mem.. 10.1101/lm.044602.116.