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Products: Primary Antibodies

Anti-ATF4 Antibody (N360A/24)

NeuroMab Logo from NeuroMab

 Datasheet

PRODUCT OPTIONS

Brand Catalog # Option Volume Concentration Price
Brand
NeuroMab Logo
Catalog #
75-345
Option
Purified
Volume
100 µL
Concentration
1 mg/mL
Price
$275.00
Brand
NeuroMab Logo
Catalog #
73-345
Option
TC Supernatant
Volume
5 mL
Concentration
Lot dependent
Price
$275.00

Validation Images


Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2a

Species Reactivity:

Human Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

ICC IHC WB

Target Description:

Cyclic AMP-dependent transcription factor ATF-4, Cyclic AMP-Responsive Element-Binding Protein 2, CREB2 or ATF4 is encoded by the gene ATF4 and is a member of the basic leucine-zipper (bZIP) superfamily. ATF4 is a transcriptional activator believed to be involved in many cell processes. ATF4 is widely expressed in tissues. In brain, ATF4 has been shown to localize in hippocampal neuron dendritic membranes. Diseases associated with ATF4 include Deafness, Mehmo Syndrome and T-Cell Leukemia.

Immunogen:

Fusion protein amino acids 25-327 (nearly full-length, all but first 24 amino acids) of human ATF4 (also known as Activating transcription factor 4, Cyclic AMP-dependent transcription factor, Cyclic AMP-responsive element-binding protein 2, CREB-2, C/EBP-related ATF, C/ATF, Taxresponsive enhancer element-binding protein 67, DNA-binding protein TAXREB67 and TXREB, accession number P18848)
Rat: 86% identity (284/327 amino acids identical)
Mouse: 85% identity (280/328 amino acids identical)
<50% identity with ATF5

Cross Reactivity:

Does not cross-react with ATF5

Protein Name(s):

Cyclic AMP-dependent transcription factor ATF-4 (cAMP-dependent transcription factor ATF-4) (Activating transcription factor 4) (Cyclic AMP-responsive element-binding protein 2) (CREB-2) (cAMP-responsive element-binding protein 2) (DNA-binding protein TAXREB67) (Tax-responsive enhancer element-binding protein 67) (TaxREB67)

Gene Name(s):

ATF4 CREB2 TXREB

Antibody Registry ID:

Purified: AB_2315807
TC Supernatant: AB_2315806


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:
This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

60 kDa


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.