Immunoblot versus crude membranes from adult rat brain (RBM) and lysates of WT and genetically engineered Vapa-/- 3A11 RAW 264.7 cells probed with N479/12 (left) or N52A/42 (right) TC supe. Cell lines courtesy of Tim Schaiff, Sanghyun Lee and Skip Virgin (Washington University, 2017 McCune et al mBio, PMID 28698274).
membrane protein-associated protein A) (VAMP-associated protein A) (33 kDa VAMPassociated
protein) (VAMP-A) (VAP-33)
VAPA and VAPB are members of the VAMP-associated protein family (VAP) (aka vesicle-associated membrane proteins) which are abundant scaffolding proteins predominantly localized to the endoplasmic reticulum (ER). VAPs have recently been shown to interact with Kv2.1 and Kv2.2 channels forming junctions to link the ER to the plasma membrane (Johnson B. et al., 2018).
Fusion protein amino acids 1-219 (N-terminus) of rat VAPA (also known as Vesicle-associated membrane protein-associated protein A, VAMP-associated protein A, 33 kDa VAMPassociated protein, VAMP-A, VAP-33 and Vap33, accession number Q9Z270)
Store at ≤ -20 C for long term storage. For short term storage, store at 2-8 C. For maximum recovery of product, centrifuge the vial prior to removing the cap.