Immunoblot versus crude membranes from adult rat brain (RBM) and lysates of WT and genetically engineered Vapa-/- 3A11 RAW 264.7 cells probed with N479/12 (left) or N52A/42 (right) TC supe. Cell lines courtesy of Tim Schaiff, Sanghyun Lee and Skip Virgin (Washington University, 2017 McCune et al mBio, PMID 28698274).
Bulk Order Anti-VAPA Antibody
VAPA and VAPB are members of the VAMP-associated protein family (VAP) (aka vesicle-associated membrane proteins) which are abundant scaffolding proteins predominantly localized to the endoplasmic reticulum (ER). VAPs have recently been shown to interact with Kv2.1 and Kv2.2 channels forming junctions to link the ER to the plasma membrane (Johnson B. et al., 2018).
Purified by Protein A chromatography
ICC, IHC, WB
Fusion protein amino acids 1-219 (N-terminus) of rat VAPA (accession number Q9Z270) produced recombinantly in E. Coli
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125
No cross-reactivity reported
Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
24 months from date of receipt
Shipped on ice packs
VAPA (Vesicle-associated membrane protein-associated protein A) (VAMP-associated protein A) (33 kDa VAMPassociatedprotein) (VAMP-A) (VAP-33)