Tissue and transfected cell immunoblot: extracts of rat brain membranes (RBM) and of COS cells transiently transfected with HA-tagged mouse and human TRPML3 (mTRPML3 and hTRPML3) or untagged Kv2.1 plasmid and probed with N268/19 (left) and K89/34 (right) TC supe.
Mucolipin-3, Transient receptor potential cation channel, mucolipin subfamily, member 3 or TrpML3 is encoded by the gene MCOLN3. It is a member of the small family of the TRPML channels, a subgroup of the large protein family of TRP ion channels and acts as an inwardly rectifying cation channel. TrpML3 is found in the cochlea, olfactory kidney, lung and hair cells and strial cells of the inner ear. The protein localizes to the plasma membrane, endosomes, and lysosomes of cells. Mutations in this gene in mice result in auditory hair cell death and deafness
Synthetic peptide amino acids 269-285 (CKDWHVSGSIQKNTHYM, extracellular N-terminus) of human TRPML3 (also known as Mucolipin-3 and MCOLN3, accession number Q8TDD5)
Mouse: 100% identity (17/17 amino acids identical)
Rat: 100% identity (17/17 amino acids identical)
Less than 50% identity with TRPML2
Human, Mouse, Rat
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