Anti-Tau Antibody

Name: Anti-Tau Antibody
Brand: Aves Labs
SKU: TAU
Price: 470.0 USD
Availability: InStock

Antipeptide antibodies were generated in chickens against two sequences shared between the mouse (P10637.3) and human (P10636.5) gene products. Neither of these sequences contain phosphorylated serine or threonine residues, so these Tau antibodies recognize both the euphosphorylated and hyperphosphorylated forms of Tau. Antibodies were affinity-purified from IgY fractions prepared from yolks collected from hyperimmunized hens. These antibodies have been validated with human, mouse and rat tissues.

Immunohistochemical presence of tau in neurofibrillary tangles in cortical neurons of an Alzheimer's Disease patient. Anti-Tau antibody (1:10,000) was incubated with formalin-fixed, paraffin-embedded sectioned material from Alzheimer's brains. Primary antibody was visualized with HRP-labeled goat anti-chicken IgY. Dr. Randy Woltjer, Oregon Health & Sciences University.

Immunohistochemical presence of tau in a neurofibrillary tangle in the remnant of a cortical neuron of an Alzheimer's Disease patient. Anti-Tau antibody (1:10,000) was incubated with formalin-fixed, paraffin-embedded sectioned material from Alzheimer's brains. Primary antibody was visualized with HRP-labeled goat anti-chicken IgY. Dr. Randy Woltjer, Oregon Health & Sciences University.

Immunohistochemical presence of tau in a neurofibrillary tangle in cortical neurons of an Alzheimer's Disease patient. Anti-Tau antibody (1:10,000) was incubated with formalin-fixed, paraffin-embedded sectioned material from Alzheimer's brains. Primary antibody was visualized with HRP-labeled goat anti-chicken IgY. Dr. Randy Woltjer, Oregon Health & Sciences University.

Immunohistochemical presence of tau in neurofibrillary tangles in cortical neurons of an Alzheimer's Disease patient. Anti-Tau antibody (1:10,000) was incubated with formalin-fixed, paraffin-embedded sectioned material from Alzheimer's brains. Primary antibody was visualized with HRP-labeled goat anti-chicken IgY. Dr. Randy Woltjer, Oregon Health & Sciences University.

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Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC

Host Species

Chicken

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Data Sheet

Trial Size

SKU: TAU

Volume: 1000 µL

Variant

Price:

$470.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Tau

Scientific Background

Human Tau protein (i.e., microtubule-associated protein tau isoform 1) is a 78,902 dalton protein (758 amino acids) expressed in cortical neurons of the CNS. In normal individuals, tau contains moderate degrees of phosphorylation on various serine and threonine residues scattered throughout the N-terminal half of this protein. However in various neurodegenerative diseases, such as Alzheimer's disease, Tau becomes hyperphosphorylated on these residues, leading to the pathological formation of intracellular "neurofibrillary tangles." Such tangles are hallmarks of Alzheimer's disease, as well as various other neurodegenerative diseases, collectively known as the "tauopathies."

Format

Affinity-Purified IgY

Concentration

200 µg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC

Host Species

Chicken

Gene Name

MAPT MAPTL MTBT1 TAU

Molecular Weight

55-62 kDa

Immunogen

Synthetic peptides

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2313563

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Physical State

Liquid

Production Notes

Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different and nonphosphorylated regions of the Tau gene product, but are shared between the human (NP_05819, NCBI) and mouse (P01637, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 200 µg/mL. Finally, equal volumes of both of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Dilution Ranges

IHC: 1:5000-1:10000
ICC: 1:5000-1:10000

Quality Control

Both of these anti-peptide antibodies were analyzed by immunohisto-chemistry (at a dilution of 1:20000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Synonyms

Microtubule-associated protein tau (Neurofibrillary tangle protein) (Paired helical filament-tau) (PHF-tau)

UniProt ID

P10636

Shipping

Ice packs

Product Specific References for Applications and Species

Immunocytochemistry: Human
Immunohistochemistry: Human
Western Blot: Mouse

Immunocytochemistry: Human
PMID	Dilution	Publication
35625761	1:500	De Kleijn, K.M.A., et al. 2022. Molecular Signature of Neuroinflammation Induced in Cytokine-Stimulated Human Cortical Spheroids. Biomedicines, .

Immunohistochemistry: Human
PMID	Dilution	Publication
21459825	1:5000	Kruer, M.C., et al. 2011. Novel histopathologic findings in molecularly-confirmed pantothenate kinase-associated neurodegeneration. Brain, 947-958.

Western Blot: Mouse
PMID	Dilution	Publication
27449203	not listed	White, J.D., et al. 2016. Hippocampal A expression, but not phosphorylated tau, predicts cognitive deficits following repeated peripheral poly I:C administration. Behavioural Brain Research, 219-225.

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Anti-Tau Antibody

SKU: TAU

Product Details

Product Specific References for Applications and Species

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