Anti-Shank2 Antibody (N23B/6)

Name: Anti-Shank2 Antibody
Brand: NeuroMab
SKU: 75-088
Price: 308.0 USD
Availability: InStock

from NeuroMab Datasheet

28 Citations

Available Conjugates

490

550

594

650

Product Type

Primary Antibody

Species Reactivity

Human, Mouse, Rabbit, Rat

Clone

N23B/6

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Format

Purified by Protein A chromatography

Gene Name

Shank2 Cortbp1

Molecular Weight

160 kDa

Go to Full Product Details

Adult rat hippocampus immunohistochemistry.

Transfected cell immunoblot: extracts of COS-1 cells transiently transfected with Shank1, Shank2 or Shank3 plasmids and probed with N23B/6 TC supe (left panel) or a monoclonal lane loading control (right panel).

Immunoblots on brain membranes prepared from whole rat (RBM) and mouse (MBM) brain, and from human cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)].

Adult rat hippocampus immunohistochemistry.

Volume: 100 µL

Variant

Price:

$308

Quantity:

Bulk Order Anti-Shank2 Antibody

Product Details

Target

Shank2

Target Description

SH3 and multiple ankyrin repeat domains protein 2, or shank 2 is a member of the shank family. Shank 2 binding domains include a SH3 domain, a proline rich domain, and a PDZ domain. Shank 2 is found in kidney, liver tissue, and epithelial cells. In brain, Shank 2 is expressed at the apical cell membrane, cytoplasm, synapse, postsynaptic density, growth cone, and dendritic spine. Some isoforms of shank 2 are expressed during development, while others are expressed in mature tissue. Shank 2 acts as a scaffold protein anchoring and connecting membrane bound receptors, including NMDA and glutamate receptors and other proteins to the cytoskeleton in neurons to enable cell signaling. In this manner, Shank 2 may play a role in synapse formation and dendritic spine maturation. Mutations in the Shank 2 gene have been associated with autism spectrm disorder and blount disease.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N23B/6

Isotype

IgG1

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

Shank2 Cortbp1

Molecular Weight

160 kDa

Antigen

Fusion protein amino acids 84-309 (SH3/PDZ domains) of rat Shank2 (accession number Q9QX74) produced recombinantly in E. Coli

Species Reactivity

Human, Mouse, Rabbit, Rat

Antibody Registry ID

AB_2254586

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.18

Dilution Ranges

WB: 1:1000
IHC: 1:500
ICC: 1:500

Conjugate

Unconjugated

Specificity

No cross-reactivity against Shank1 or Shank3

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

SH3 and multiple ankyrin repeat domains protein 2 (Shank2) (Cortactin-binding protein 1) (CortBP1) (GKAP/SAPAP-interacting protein) (Proline-rich synapse-associated protein 1) (ProSAP1) (SPANK-3)

UniProt Details

UniProt (Human): Q9UPX8
UniProt (Immunogen Species): Q9QX74

Product Specific References for Applications and Species

Immunocytochemistry: Mouse | Rat
ImmunoGold Labeling: Rat
Immunohistochemistry: Mouse
Western Blot: Human | Mouse | Rabbit| Rat

Immunocytochemistry: Mouse
PMID	Dilution	Publication
32275651	not listed	Willems, J., et al. 2020. ORANGE: A CRISPR/Cas9-based genome editing toolbox for epitope tagging of endogenous proteins in neurons. PLoS Biology, e3000665.
29478916	1:500	Mikhaylova, M., et al. 2018. Caldendrin Directly Couples Postsynaptic Calcium Signals to Actin Remodeling in Dendritic Spines. Neuron, 1110-1125.
24153177	not listed	Han, K., et al. 2013. SHANK3 overexpression causes manic-like behaviour with unique pharmacogenetic properties. Nature, 72-77.
21994763	1:500	Berkel, S., et al. 2012. Inherited and de novo SHANK2 variants associated with autism spectrum disorder impair neuronal morphogenesis and physiology. Human molecular genetics, 344-357.

Immunocytochemistry: Rat
PMID	Dilution	Publication
30524232	1:100	Ha, H., et al. 2018. Shank and Zinc Mediate an AMPA Receptor Subunit Switch in Developing Neurons. Frontiers in molecular neuroscience, 405.
29735556	1:1000	Campbell, M.K., et al. 2018. USP8 Deubiquitinates SHANK3 to Control Synapse Density and SHANK3 Activity-Dependent Protein Levels. The Journal of neuroscience : the official journal of the Society for Neuroscience, 5289-5301.
29478916	1:500	Mikhaylova, M., et al. 2018. Caldendrin Directly Couples Postsynaptic Calcium Signals to Actin Remodeling in Dendritic Spines. Neuron, 1110-1125.
26547831	1:400	MacGillavry, H., et al. 2016. Shank-cortactin interactions control actin dynamics to maintain flexibility of neuronal spines and synapses. The European journal of neuroscience, 179-193.
24239382	not listed	Baple, E., et al. 2014. Mutations in KPTN cause macrocephaly, neurodevelopmental delay, and seizures. American journal of human genetics, 87-94.
21795692	not listed	Verpelli, C., et al. 2011. Importance of Shank3 protein in regulating metabotropic glutamate receptor 5 (mGluR5) expression and signaling at synapses. The Journal of biological chemistry, 34869-34850.

ImmunoGold Labeling: Rat
PMID	Dilution	Publication
32238193	1:200	Tao-Cheng, J.H, et al. 2020. Activity-dependent redistribution of CaMKII in the postsynaptic compartment of hippocampal neurons. Molecular brain, 53.
26665164	1:200	Tao-Cheng, J.H., et al. 2015. Depolarization of Hippocampal Neurons Induces Formation of Nonsynaptic NMDA Receptor Islands Resembling Nascent Postsynaptic Densities. eNeuro, ENEURO.0066-15.20015.
26215919	1:50	Farley, M., et al. 2015. Electron tomographic structure and protein composition of isolated rat cerebellar, hippocampal and cortical postsynaptic densities. Neuroscience, 286-301.
25775468	1:200	Tao-Cheng, J.H., et al. 2015. Differential distribution of Shank and GKAP at the postsynaptic density. PLoS one, e0118750.
20347015	1:200	Tao-Cheng, J.H., et al. 2010. Activity induced changes in the distribution of Shanks at hippocampal synapses. Neuroscience, 44882.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
28134307	1:500	Joshi, P., et al. 2017. Fingolimod Limits Acute Aβ Neurotoxicity and Promotes Synaptic Versus Extrasynaptic NMDA Receptor Functionality in Hippocampal Neurons. Scientific reports, 41734.
25637745	not listed	Braude, J., et al. 2015. Deletion of Shank1 has minimal effects on the molecular composition and function of glutamatergic afferent postsynapses in the mouse inner ear. Hearing research, 52-64.

Western Blot: Human
PMID	Dilution	Publication
25560758	not listed	Peykov, S., et al. 2015. Identification and functional characterization of rare SHANK2 variants in schizophrenia. Molecular psychiatry, 1489-1498.

Western Blot: Mouse
PMID	Dilution	Publication
24751538	not listed	Schneider, K., et al. 2014. ProSAP1 and membrane nanodomain-associated syndapin I promote postsynapse formation and function. The Journal of cellular biology, 197-215.
24153177	not listed	Han, K., et al. 2013. SHANK3 overexpression causes manic-like behaviour with unique pharmacogenetic properties. Nature, 72-77.
22338020	not listed	Qian, H., et al. 2012. β2-Adrenergic receptor supports prolonged theta tetanus-induced LTP. Journal of neurophysiology, 2703-2712.
21551375	not listed	Xie, M., et al. 2011. Inactivation of multidrug resistance proteins disrupts both cellular extrusion and intracellular degradation of cAMP. Molecular pharmacology, 281-293.

Western Blot: Rabbit
PMID	Dilution	Publication
22338020	not listed	Qian, H., et al. 2012. β2-Adrenergic receptor supports prolonged theta tetanus-induced LTP. Journal of neurophysiology, 2703-2712.

Western Blot: Rat
PMID	Dilution	Publication
30524232	1:1000	Ha, H., et al. 2018. Shank and Zinc Mediate an AMPA Receptor Subunit Switch in Developing Neurons. Frontiers in molecular neuroscience, 405.
29735556	1:1000	Campbell, M.K., et al. 2018. USP8 Deubiquitinates SHANK3 to Control Synapse Density and SHANK3 Activity-Dependent Protein Levels. The Journal of neuroscience : the official journal of the Society for Neuroscience, 5289-5301.
27144302	1:1000	Tao-Cheng, J.H., et al. 2016. Zinc Stabilizes Shank3 at the Postsynaptic Density of Hippocampal Synapses. PLoS one, e0153979.
21795692	not listed	Verpelli, C., et al. 2011. Importance of Shank3 protein in regulating metabotropic glutamate receptor 5 (mGluR5) expression and signaling at synapses. The Journal of biological chemistry, 34869-34850.
20347015	1:1000	Tao-Cheng, J.H., et al. 2010. Activity induced changes in the distribution of Shanks at hippocampal synapses. Neuroscience, 44882.

Recover password

Create my account

Anti-Shank2 Antibody (N23B/6)

Available Conjugates

Anti-Shank2 Antibody
SKU: 75-088

Bulk Order Anti-Shank2 Antibody

Product Details

Product Specific References for Applications and Species

Related Products

Recently Viewed

Anti-Shank2 Antibody (N23B/6)

Available Conjugates

Anti-Shank2 Antibody SKU: 75-088

Bulk Order Anti-Shank2 Antibody

Product Details

Product Specific References for Applications and Species

Anti-Shank2 Antibody
SKU: 75-088