Anti-Shank2 Antibody (N23B/6)

from NeuroMab Datasheet
29 Citations

Available Conjugates

490
550
594
650

Primary Antibody
Human, Mouse, Rabbit, Rat
N23B/6
ICC, IHC, IP, WB
Mouse
Purified by Protein A chromatography
Shank2 Cortbp1
160 kDa

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Adult rat hippocampus immunohistochemistry.
Transfected cell immunoblot: extracts of COS-1 cells transiently transfected with Shank1, Shank2 or Shank3 plasmids and probed with N23B/6 TC supe (left panel) or a monoclonal lane loading control (right panel).
Immunoblots on brain membranes prepared from whole rat (RBM) and mouse (MBM) brain, and from human cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)].
Swollen dystrophic neurites of 5xFAD; Thy1-YFP mice at six months of age were immunostained for synaptotagmin-1 (STG1), and Shank2 (cat. 75-088) to co-stain pre and postsynaptic compartments, respectively. Neurofilament (NF) was used to label dystrophic neurites. Image from publication CC-BY-4.0. PMID:37667395
Lack of swollen dystrophic neurites in the molecular layers of the dentate gyrus rich in YFP-positive dendrites of dentate granule neurons at seven months of age of 5xFAD; Thy1-YFP mice. Sections were immunostained for Aβ, and presynaptic markers such as vesicular glutamate transporter 1 (vGlut1), and Tau, and postsynaptic markers such as Shank2 (cat. 75-088), and Map2. Image from publication CC-BY-4.0. PMID:37667395
Adult rat hippocampus immunohistochemistry.
Adult rat hippocampus immunohistochemistry.
Transfected cell immunoblot: extracts of COS-1 cells transiently transfected with Shank1, Shank2 or Shank3 plasmids and probed with N23B/6 TC supe (left panel) or a monoclonal lane loading control (right panel).
Immunoblots on brain membranes prepared from whole rat (RBM) and mouse (MBM) brain, and from human cerebral cortex [HBM(Cx)] and hippocampus [HBM(H)].
Swollen dystrophic neurites of 5xFAD; Thy1-YFP mice at six months of age were immunostained for synaptotagmin-1 (STG1), and Shank2 (cat. 75-088) to co-stain pre and postsynaptic compartments, respectively. Neurofilament (NF) was used to label dystrophic neurites. Image from publication CC-BY-4.0. PMID:37667395
Lack of swollen dystrophic neurites in the molecular layers of the dentate gyrus rich in YFP-positive dendrites of dentate granule neurons at seven months of age of 5xFAD; Thy1-YFP mice. Sections were immunostained for Aβ, and presynaptic markers such as vesicular glutamate transporter 1 (vGlut1), and Tau, and postsynaptic markers such as Shank2 (cat. 75-088), and Map2. Image from publication CC-BY-4.0. PMID:37667395

Anti-Shank2 Antibody
SKU: 75-088

Volume: 100 µL
1-2 business days
Price:
$308

Bulk Order Anti-Shank2 Antibody

Product Specific References for Applications and Species

Immunocytochemistry: Mouse
PMID Dilution Publication
32275651not listedWillems, J., et al. 2020. ORANGE: A CRISPR/Cas9-based genome editing toolbox for epitope tagging of endogenous proteins in neurons. PLoS Biology, e3000665.
294789161:500Mikhaylova, M., et al. 2018. Caldendrin Directly Couples Postsynaptic Calcium Signals to Actin Remodeling in Dendritic Spines. Neuron, 1110-1125.
24153177not listedHan, K., et al. 2013. SHANK3 overexpression causes manic-like behaviour with unique pharmacogenetic properties. Nature, 72-77.
219947631:500Berkel, S., et al. 2012. Inherited and de novo SHANK2 variants associated with autism spectrum disorder impair neuronal morphogenesis and physiology. Human molecular genetics, 344-357.
Immunocytochemistry: Rat
PMID Dilution Publication
305242321:100Ha, H., et al. 2018. Shank and Zinc Mediate an AMPA Receptor Subunit Switch in Developing Neurons. Frontiers in molecular neuroscience, 405.
297355561:1000Campbell, M.K., et al. 2018. USP8 Deubiquitinates SHANK3 to Control Synapse Density and SHANK3 Activity-Dependent Protein Levels. The Journal of neuroscience : the official journal of the Society for Neuroscience, 5289-5301.
294789161:500Mikhaylova, M., et al. 2018. Caldendrin Directly Couples Postsynaptic Calcium Signals to Actin Remodeling in Dendritic Spines. Neuron, 1110-1125.
265478311:400MacGillavry, H., et al. 2016. Shank-cortactin interactions control actin dynamics to maintain flexibility of neuronal spines and synapses. The European journal of neuroscience, 179-193.
24239382not listedBaple, E., et al. 2014. Mutations in KPTN cause macrocephaly, neurodevelopmental delay, and seizures. American journal of human genetics, 87-94.
21795692not listedVerpelli, C., et al. 2011. Importance of Shank3 protein in regulating metabotropic glutamate receptor 5 (mGluR5) expression and signaling at synapses. The Journal of biological chemistry, 34869-34850.
ImmunoGold Labeling: Rat
PMID Dilution Publication
322381931:200Tao-Cheng, J.H, et al. 2020. Activity-dependent redistribution of CaMKII in the postsynaptic compartment of hippocampal neurons. Molecular brain, 53.
266651641:200Tao-Cheng, J.H., et al. 2015. Depolarization of Hippocampal Neurons Induces Formation of Nonsynaptic NMDA Receptor Islands Resembling Nascent Postsynaptic Densities. eNeuro, ENEURO.0066-15.20015.
262159191:50Farley, M., et al. 2015. Electron tomographic structure and protein composition of isolated rat cerebellar, hippocampal and cortical postsynaptic densities. Neuroscience, 286-301.
257754681:200Tao-Cheng, J.H., et al. 2015. Differential distribution of Shank and GKAP at the postsynaptic density. PLoS one, e0118750.
203470151:200Tao-Cheng, J.H., et al. 2010. Activity induced changes in the distribution of Shanks at hippocampal synapses. Neuroscience, 44882.
Immunohistochemistry: Mouse
PMID Dilution Publication
376673951:300-1:1000Jang, J, et al. 2023. Abnormal accumulation of extracellular vesicles in hippocampal dystrophic axons and regulation by the primary cilia in Alzheimer's disease. Acta Neuropathologica Communications, 142.
281343071:500Joshi, P., et al. 2017. Fingolimod Limits Acute Aβ Neurotoxicity and Promotes Synaptic Versus Extrasynaptic NMDA Receptor Functionality in Hippocampal Neurons. Scientific reports, 41734.
25637745not listedBraude, J., et al. 2015. Deletion of Shank1 has minimal effects on the molecular composition and function of glutamatergic afferent postsynapses in the mouse inner ear. Hearing research, 52-64.
Western Blot: Human
PMID Dilution Publication
25560758not listedPeykov, S., et al. 2015. Identification and functional characterization of rare SHANK2 variants in schizophrenia. Molecular psychiatry, 1489-1498.
Western Blot: Mouse
PMID Dilution Publication
24751538not listedSchneider, K., et al. 2014. ProSAP1 and membrane nanodomain-associated syndapin I promote postsynapse formation and function. The Journal of cellular biology, 197-215.
24153177not listedHan, K., et al. 2013. SHANK3 overexpression causes manic-like behaviour with unique pharmacogenetic properties. Nature, 72-77.
22338020not listedQian, H., et al. 2012. β2-Adrenergic receptor supports prolonged theta tetanus-induced LTP. Journal of neurophysiology, 2703-2712.
21551375not listedXie, M., et al. 2011. Inactivation of multidrug resistance proteins disrupts both cellular extrusion and intracellular degradation of cAMP. Molecular pharmacology, 281-293.
Western Blot: Rabbit
PMID Dilution Publication
22338020not listedQian, H., et al. 2012. β2-Adrenergic receptor supports prolonged theta tetanus-induced LTP. Journal of neurophysiology, 2703-2712.
Western Blot: Rat
PMID Dilution Publication
305242321:1000Ha, H., et al. 2018. Shank and Zinc Mediate an AMPA Receptor Subunit Switch in Developing Neurons. Frontiers in molecular neuroscience, 405.
297355561:1000Campbell, M.K., et al. 2018. USP8 Deubiquitinates SHANK3 to Control Synapse Density and SHANK3 Activity-Dependent Protein Levels. The Journal of neuroscience : the official journal of the Society for Neuroscience, 5289-5301.
271443021:1000Tao-Cheng, J.H., et al. 2016. Zinc Stabilizes Shank3 at the Postsynaptic Density of Hippocampal Synapses. PLoS one, e0153979.
21795692not listedVerpelli, C., et al. 2011. Importance of Shank3 protein in regulating metabotropic glutamate receptor 5 (mGluR5) expression and signaling at synapses. The Journal of biological chemistry, 34869-34850.
203470151:1000Tao-Cheng, J.H., et al. 2010. Activity induced changes in the distribution of Shanks at hippocampal synapses. Neuroscience, 44882.

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