Anti-Presenilin 2 loop region Antibody

Name: Anti-Presenilin 2 loop region Antibody
Brand: Biosensis
SKU: R-1681-500
Price: 337.0 USD
Availability: InStock

Our Anti-Presenilin 2 loop region rabbit polyclonal primary antibody detects human and mouse Presenilin 2 loop region, and is IgG. It is validated for use in ICC, WB.

Western Immunoblotting of mouse and human Presenilin 2 protein in mouse cell line extracts, various mouse tissues and a human cell line. Membrane proteins were prepared and loaded as 20 µg protein per lane. Crude anti-PS2 loop antibody used at 1:1000.

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Species Reactivity

Human, Mouse

Applications

ICC, WB

Host Species

Rabbit

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Data Sheet

SKU: R-1681-500

Volume/Size: 500 µg

Variant

Price:

$337.00

Quantity:

Product Details

Target

Presenilin 2 loop region

Scientific Background

Autosomal dominant mutations in presenilin 2 are the second major cause of early-onset familial Alzheimer's disease. Presenilin 2 is a multi-transmembrane protein which undergoes endoprotelysis to form an N-terminal fragment of about 29 kDa and C-terminal fragment of about 22 kDa. Presenilin 2 forms the catalytic core of the gamma-secretase complex which cleaves type 1 transmembrane proteins including the amyloid precursor protein to generate the C-terminus of the amyloid beta peptide.

Format

IgG

Clonality

Polyclonal

Isotype

IgG

Applications

ICC, WB

Host Species

Rabbit

Molecular Weight

22 kDa, 45 kDa (See application details.)

Immunogen

A synthetic peptide (KLDPSSQGALQLPYDPEMEEDSYDSFGEP-C) corresponding to human PS1 [306-334] in the loop region conjugated via additional C-terminal Cys to Diphtheria toxoid.

Immunogen Species

Human

Species Reactivity

Human, Mouse

Storage

Spin vial briefly before opening. Reconstitute in 500 µL sterile-filtered 1X PBS, pH 7.2-7.6. Centrifuge to remove any insoluble material. Short term storage at 2-8°C for one week. At -20°C as an undiluted liquid for up to 12 months.

Physical State

Lyophilized

Production Notes

Protein G purified IgG

Buffer

Lyophilized from PBS, pH 7.4. Contains no preservative.

Dilution Ranges

WB: 1:1000
IP: 1:100

Application Details

Full length presenilin 2 (448 aa) has relative MW of about 45 kDa, with this antibody most commonly detected as cleaved CTF of 22 kDa with this antibody. Human or mouse brain samples commonly prepared with reducing agent (50 mM DTT), urea (2.3 M), SDS (1.5%) in 62.5 mM Tris-HCL pH 6.8 sample buffer (without boiling) heating to 50 C for 15 min.

Conjugate

Unconjugated

Specificity

Confirmed by Western blot using mouse and human brain and knock down of presenilin 2 in vitro using siRNA see ref 6 below. Not reactive with presenilin 1.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

AD3LP, AD5, E5-1, STM-2

UniProt ID

P49810

Shipping

25°C (ambient)

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Anti-Presenilin 2 loop region Antibody

SKU: R-1681-500

Product Details

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