Our Anti-Nuclear Pore Complex primary antibody from PhosphoSolutions is mouse monoclonal. It detects bovine, chicken, drosophila, human, mouse, and rat Nuclear pore Complex and is concentrated tissue culture supernatant. It is great for use in ICC.
Immunostaining of HeLa cells stained with anti-nuclear pore complex antibody (cat. 1515-NPC, green, 1:100) and rabbit anti-vimentin antibody (cat. 2105-VIM, red, 1:500).
The nuclear pore complex (NPC) is a very large structure made up of at least 50 different proteins that span the double membrane of the nuclear envelope functioning as a gateway for macromolecular traffic between the cytoplasm and the nucleus (Nakielny and Dreyfuss,1999). Discrete nuclear pore complex proteins or nucleoporins such as NUP98, NUP180 and p62 have been implicated in autoimmune disease and cancer. Patients with primary biliary cirrhosis (PBC) frequently produce autoantibodies against p62 and NUP180 (Wilken et al., 1993; Nesher et al., 2001) while NUP98 translocations have been found in patients with acute myelogenous leukemia (AML) (Jaju et al. 2001)
Concentrated tissue culture supernatant
Monoclonal
39C7
IgG1
ICC
Mouse
Nuclear stain of multiple gene products including Nup62, Nup133
Yeast nuclear preparations
Human, Mouse, Rat
AB_2492188
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Liquid
Concentrated tissue culture supernatant.
Concentrated tissue culture supernatent + 10 mM NaN3.
Specific for endogenous levels of the nuclear pore complex proteins.
Immunofluorescence performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Nakielny S, Dreyfuss G (1999) Transport of proteins and RNAs in and out of the nucleus. Cell 99(7):677-690. PMID: 10619422
Wilken N, Kossner U, Senecal JL, Scheer U, Dabauvalle MC (1993) Nup180, a novel nuclear pore complex protein localizing to the cytoplasmic ring and associated fibrils. Journal of Cell Biology 123(6 pt 1):1345-54. PMID: 8253835
Nesher G, Margalit R, Ashkenazi YJ (2001) Anti-nuclear envelope antibodies: Clinical associations. Seminars in Arthritis and Rheumatism 30(5):313-20. PMID: 11303304
Jaju RJ, Fidler C, Haas OA, Strickson AJ, Watkins F, Clark K, Cross NC, Cheng JF, Aplan PD, Kearney L, Boultwood J, Wainscoat JS (2001) A novel gene, NSD1, is fused to NUP98 in the t(5;11)(q35;p15.5) in de novo childhood acute myeloid leukemia. Blood 98(4):1264-7. PMID: 11493482
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