Our Anti-NMDA NR2C Subunit rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat NMDA NR2C Subunit and is antigen affinity purified from pooled serum. It is great for use in WB, IP.
Western blot of 10 µg of rat cerebellar lysate showing specific immunolabeling of the ~140 kDa NR2C subunit of the NMDA receptor.
The ion channels activated by glutamate that are sensitive to N-methyl-Daspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits. The NR2C subunit of the receptor is thought to influence the NMDAR conductance level (Ebralidze et al., 1996).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
IP, WB
Rabbit
GRIN2C
140 kDa
Fusion protein from the N-terminal region of the NR2C subunit of the rat NMDA receptor.
Human, Mouse, Rat
AB_2492184
Reconstitute in 50 µl phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use. After reconstitution it is recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Lyophilized
Prepared from pooled rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.
Specific for endogenous levels of the ~140 kDa NR2C subunit of the NMDA receptor. Also labels the ~180 kDa NR2A and the ~180 kDa NR2B subunits of the NMDA receptor. Immunolabeling is blocked by preadsorption of antibody with the immunogen that was used to generate the antibody.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
GluN2C antibody, Glutamate [NMDA] receptor subunit epsilon-3 antibody, Glutamate Receptor Ionotropic N Methyl D Aspartate 2C antibody, Glutamate receptor ionotropic NMDA 2C antibody, Glutamate receptor ionotropic N methyl D aspartate 2C antibody, Grin2c antibody, Grin 2c antibody, GRIN2C antibody, GRIN 2C antibody, N Methly D Aspartate Receptor Channel Subunit Epsilon 3 antibody, N Methyl D Aspartate Receptor Subtype 2C antibody, N methyl D aspartate receptor subunit 2C antibody, N-methyl D-aspartate receptor subtype 2C antibody, NMDAR2C antibody, NMDE3_HUMAN antibody, NR2C antibody, OTTHUMP00000041930 antibody
Alvestad RM, Grosshans DR, Coultrap SJ, Nakazawa T, Yamamoto T, Browning MD** (2003) Tyrosine dephosphorylation and ethanol inhibition of N-methyl-D-aspartate receptor function. Journal of Biological Chemistry 278:11020-11025. PMID: 12536146
Carroll RC, Zukin RS (2002) NMDA-receptor trafficking and targeting: implications for synaptic transmission and plasticity. Trends in Neuroscience25:571-577. PMID: 12392932
Ebralidze, A.K., Rossi, D.J., Tonegawa, S. and Slater, N.T., 1996. Modification of NMDA receptor channels and synaptic transmission by targeted disruption of the NR2C gene. Journal of Neuroscience, 16(16), pp.5014-5025. PMID: 8756432
Grosshans DR, Clayton DA, Coultrap SJ, Browning MD** (2002) LTP leads to rapid surface expression of NMDA but not AMPA receptors in adult rat CA1. Nature Neuroscience 5:27-33. PMID: 11740502
Lovinger DM, White G, Weight FF (1989) Ethanol inhibits NMDA-activated ion current in hippocampal neurons. Science 243:1721-1724. PMID: 2467382
Lu W-Y, Xiong Z-G, Lei S, Orser BA, Browning MD**, MacDonald JF (1999) G-protein coupled receptors act via protein kinase C and Src to regulate NMDA receptors. Nature Neuroscience 2:331-338. PMID: 10204539
Snell LD, Nunley KR, Lickteig RL, Browning MD**, Tabakoff B, Hoffman PL (1996) Regional and subunit specific changes in NMDA receptor mRNA and immunoreactivity in mouse brain following chronic ethanol ingestion. Molecular Brain Research 40:71-78. PMID: 8840015
Wenthold RJ, Prybylowski K, Standley S, Sans N, Petralia RS (2003) Trafficking of NMDA receptors. Annual Reviews of Pharmacology and Toxicology 43:335-358. PMID: 12540744
**Dr. Michael Browning, a co-author of the above cited papers, is CEO and founder of PhosphoSolutions.
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