Anti-NMDA NR2C Subunit Antibody

Name: Anti-NMDA NR2C Subunit Antibody
Brand: PhosphoSolutions
SKU: 1502-NR2C
Price: 456.0 USD
Availability: InStock

Our Anti-NMDA NR2C Subunit rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat NMDA NR2C Subunit and is antigen affinity purified from pooled serum. It is great for use in WB, IP.

Western blot of 10 µg of rat cerebellar lysate showing specific immunolabeling of the ~140 kDa NR2C subunit of the NMDA receptor.

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, IP, WB

Host Species

Rabbit

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Data Sheet

Pooled Serum

SKU: 1502-NR2C

Size: 10 µg

Variant

Price:

$456.00

Quantity:

Ships: 1-2 business days

Product Details

Target

NMDA NR2C Subunit

Scientific Background

The ion channels activated by glutamate that are sensitive to N-methyl-Daspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell death (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). The NMDA receptor is also one of the principal molecular targets for alcohol in the CNS (Lovinger et al., 1989; Alvestad et al., 2003; Snell et al., 1996). The NMDAR is also potentiated by protein phosphorylation (Lu et al., 1999). The rat NMDAR1 (NR1) was the first subunit of the NMDAR to be cloned. The NR1 protein can form NMDA activated channels when expressed in Xenopus oocytes but the currents in such channels are much smaller than those seen in situ. Channels with more physiological characteristics are produced when the NR1 subunit is combined with one or more of the NMDAR2 (NR2 A-D) subunits. The NR2C subunit of the receptor is thought to influence the NMDAR conductance level (Ebralidze et al., 1996).

Format

Antigen Affinity Purified from Pooled Serum

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, IP, WB

Host Species

Rabbit

Gene Name

GRIN2C

Molecular Weight

140 kDa

Immunogen

Fusion protein from the N-terminal region of the NR2C subunit of the rat NMDA receptor.

Immunogen Species

Rat

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2492184

Storage

Reconstitute in 50 µl phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use. After reconstitution it is recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Lyophilized

Production Notes

Prepared from pooled rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.

Buffer

Lyophilized

Dilution Ranges

WB: 1:1000
WB Brain: 1:1000
IP: 3 µl per 200 µg lysate

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~140 kDa NR2C subunit of the NMDA receptor. Also labels the ~180 kDa NR2A and the ~180 kDa NR2B subunits of the NMDA receptor. Immunolabeling is blocked by preadsorption of antibody with the immunogen that was used to generate the antibody.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

GluN2C; Glutamate [NMDA receptor subunit epsilon-3; Glutamate Receptor Ionotropic N Methyl D Aspartate 2C; Glutamate receptor ionotropic NMDA 2C; Grin2c; Grin 2c; N Methly D Aspartate Receptor Channel Subunit Epsilon 3; N Methyl D Aspartate Receptor Subtype 2C; N methyl D aspartate receptor subunit 2C; N-methyl D-aspartate receptor subtype 2C; NMDAR2C; NMDE3_HUMAN; NR2C; OTTHUMP00000041930

UniProt ID

Q00961
UniProt Summary: Component of N-methyl-D-aspartate (NMDA) receptors (NMDARs) that function as heterotetrameric, ligand-gated cation channels with high calcium permeability and voltage-dependent block by Mg(2+) (PubMed:26875626, PubMed:36309015). Participates in synaptic plasticity for learning and memory formation by contributing to the slow phase of excitatory postsynaptic current and long-term synaptic potentiation (By similarity). Channel activation requires binding of the neurotransmitter L-glutamate to the GluN2 subunit, glycine or D-serine binding to the GluN1 subunit, plus membrane depolarization to eliminate channel inhibition by Mg(2+) (PubMed:26875626, PubMed:36309015). NMDARs mediate simultaneously the potassium efflux and the influx of calcium and sodium (By similarity). Each GluN2 subunit confers differential attributes to channel properties, including activation, deactivation and desensitization kinetics, pH sensitivity, Ca2(+) permeability, and binding to allosteric modulators (PubMed:26875626). {ECO:0000250|UniProtKB:P35438, ECO:0000250|UniProtKB:Q01098, ECO:0000269|PubMed:26875626, ECO:0000269|PubMed:36309015}.

NCBI Gene ID

24411

Shipping

Blue Ice

Western Blot Protocol: Download

General References

Alvestad RM, Grosshans DR, Coultrap SJ, Nakazawa T, Yamamoto T, Browning MD** (2003) Tyrosine dephosphorylation and ethanol inhibition of N-methyl-D-aspartate receptor function. Journal of Biological Chemistry 278:11020-11025. PMID: 12536146
Carroll RC, Zukin RS (2002) NMDA-receptor trafficking and targeting: implications for synaptic transmission and plasticity. Trends in Neuroscience25:571-577. PMID: 12392932
Ebralidze, A.K., Rossi, D.J., Tonegawa, S. and Slater, N.T., 1996. Modification of NMDA receptor channels and synaptic transmission by targeted disruption of the NR2C gene. Journal of Neuroscience, 16(16), pp.5014-5025. PMID: 8756432
Grosshans DR, Clayton DA, Coultrap SJ, Browning MD** (2002) LTP leads to rapid surface expression of NMDA but not AMPA receptors in adult rat CA1. Nature Neuroscience 5:27-33. PMID: 11740502
Lovinger DM, White G, Weight FF (1989) Ethanol inhibits NMDA-activated ion current in hippocampal neurons. Science 243:1721-1724. PMID: 2467382
Lu W-Y, Xiong Z-G, Lei S, Orser BA, Browning MD**, MacDonald JF (1999) G-protein coupled receptors act via protein kinase C and Src to regulate NMDA receptors. Nature Neuroscience 2:331-338. PMID: 10204539
Snell LD, Nunley KR, Lickteig RL, Browning MD**, Tabakoff B, Hoffman PL (1996) Regional and subunit specific changes in NMDA receptor mRNA and immunoreactivity in mouse brain following chronic ethanol ingestion. Molecular Brain Research 40:71-78. PMID: 8840015
Wenthold RJ, Prybylowski K, Standley S, Sans N, Petralia RS (2003) Trafficking of NMDA receptors. Annual Reviews of Pharmacology and Toxicology 43:335-358. PMID: 12540744
**Dr. Michael Browning, a co-author of the above cited papers, is CEO and founder of PhosphoSolutions.

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Anti-NMDA NR2C Subunit Antibody

SKU: 1502-NR2C

Product Details

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