Anti-NGFR/p75 neurotrophin receptor (p75NTR) Antibody (ATTO 488) (8J2)

Name: Anti-NGFR/p75 neurotrophin receptor (p75NTR) Antibody (ATTO 488)
Brand: Biosensis
SKU: M-1821-50-AT
Price: 217.0 USD
Availability: InStock

Our Anti-NGFR/p75 neurotrophin receptor (p75NTR) mouse monoclonal primary antibody detects human, mouse, and rat NGFR/p75 neurotrophin receptor (p75NTR), and is IgG. It is validated for use in FC, ICC.

Trafficking of internalized NGFR/p75 receptor with p75 antibody clone 8J2, conjugated with ATTO 488 (M-1821-50-AT, green). Human SH-SY5Y cells were incubated for 1 hour with 2 µg/mL 8J2-ATTO 488 at 37C, fixed (4% formaldehyde), and cell nuclei stained with Hoechst dye (blue). Confocal images (40x magnification) demonstrate typical vesicle-like, punctuate perinuclear staining of internalized receptor/antibody.

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Species Reactivity

Human, Mouse, Rat

Applications

Flow, ICC

Host Species

Mouse

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Data Sheet

SKU: M-1821-50-AT

Volume/Size: 50 µg

Variant

Price:

$217.00

Quantity:

Product Details

Target

NGFR/p75 neurotrophin receptor (p75NTR)

Scientific Background

p75NTR (CD271) was originally discovered as a low affinity nerve growth factor receptor (NGFR). Later it was found that it was the receptor for all neurotrophins, including NGF, BDNF, NT3 and NT4/5. It mediates signals of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. Recently, it has been revealed that p75NTR not only acts as the receptor for neurotrophins but also the receptor for many other pathological ligands such as prions, rabies virus and amyloid beta. p75NTR also acts as a co-receptor for NOGO which mediates inhibitory signals of myelin associated protein. p75NTR is highly expressed in a number of non-neuronal and neuronal cells including motor neurons during development and also in damaged neurons. Recent research proposes the extracellular domain of p75NTR as a biomarker for monitoring the progression of motor neuron disease (MND), also known as Amyotrophic Lateral Sclerosis (ALS) or Lou Gehrig's Disease. SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with NGFRAP1/BEX3.

Format

IgG

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

8J2

Isotype

IgG2a

Applications

Flow, ICC

Host Species

Mouse

Immunogen

Recombinant extracellular domain (amino acids 29-250) of human NGFR/p75NTR protein with N-terminal His-tag.

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Storage

Liquid antibody is shipped cold. Upon arrival, spin vial briefly, divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.

Physical State

Liquid

Production Notes

Protein A purified IgG was labelled with ATTO 488 and free dye removed by gel filtration.

Buffer

PBS, pH 7.2-7.6, without preservative. Typical Fluorophore/Protein (F/P) - ratio is 3-10.

Dilution Ranges

ICC: 1-5 µg/mL

Application Details

ICC: Light fixation only, or unfixed works best. Epitope is sensitive to fixation.
Flow Cytometry: 5-20 µg/mL.

Conjugate

ATTO 488 Ex: 500 nm, EM: 520 nm

Specificity

Reacts with human, mouse and rat. Cross-reactivity with other species not tested but expected.This antibody is specific for NGFR/p75NTR as demonstrated by Western blotting and immunprecipitation. The antibody recognizes extracellular p75NTR under non-reducing conditions.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

4 months after date of receipt (unopened vial)

Synonyms

Low-affinity nerve growth factor receptor; NGF receptor; Gp80-LNGFR; p75 ICD; Low affinity neurotrophin receptor p75NTR; CD271

UniProt ID

P08138

Shipping

2-8°C (on cold packs)

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Anti-NGFR/p75 neurotrophin receptor (p75NTR) Antibody (ATTO 488) (8J2)

SKU: M-1821-50-AT

Product Details

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